Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,843,875	G→C	9.3%	L241L (CTG→CTC) ‡	PP_4259 →	iron‑sulfur cluster‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,843,875	0	G	C	9.3%	129.5 / 4.3	43	L241L (CTG→CTC) ‡	PP_4259	iron‑sulfur cluster‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (22/17);  new base C (2/2);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.47e-01

AGGCCGATGGCTGGGCCTACTTCTGGGTCGGCTTCTTCACCCTCGCCACTTACGGCAACGCCGGCTGGCTACGCGAACAGGTGTGCGTGTACATGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCATCGACTGCACCATGTGCGTGCAGGTCTGCCCGACCGGCATCGACATCCGTGACGG  >  NC_002947/4843733‑4844018                                                                                                                                                |                                                                                                                                                aGGCCGATGGCTGGGCCTACTTCTGGGTCGGCTTCTTCACCCTCGCCACTTACGGCAACGCCGGCTGGCTACGCGAACAGGTGTGCGTGTACATGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCgtg                                                                                                                                           >  4:67185/1‑149 (MQ=255)     cGATGGCTGGGCCTACTTCTGGGTCGGCTTCTTCACCCTCGCCACTTACGGCAACGCCGGCTGGCTACGCGAACAGGTGTGCGTGTACATGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCt                                                                                                                                       <  8:282893/149‑1 (MQ=255)            tGGGCCTACTTCTGGGTCGGCTTCTTCACCCTCGCCACTTACGGCAACGCCGGCTGGCTACGCGAACAGGTGTGCGTGTACATGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCgtg                                                                                                                                           >  4:10959/1‑138 (MQ=255)            tGGGCCTACTTCTGGGTCGGCTTCTTCACCCTCGCCACTTACGGCAACGCCGGCTGGCTACGCGAACAGGTGTGCGTGTACATGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCgtg                                                                                                                                           <  3:10959/138‑1 (MQ=255)                ccTACTTCTGGGTCGGCTTCTTCACCCTCGCCACTTACGGCAACGCCGGCTGGCTACGCGAACAGGTGTGCGTGTACATGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGc                                                                                                                            >  1:138087/1‑149 (MQ=255)                ccTACTTCTGGGTCGGCTTCTTCACCCTCGCCACTTACGGCAACGCCGGCTGGCTACGCGAACAGGTGTGCGTGTACATGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGc                                                                                                                            >  6:11589/1‑149 (MQ=255)                 cTACTTCTGGGTCGGCTTCTTCACCCTCGCCACTTACGGCAACGACGGCTGGCTACGCGAACAGGTGTGCGTGTACATGTGCACCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATc                                                                                                                                              >  7:538/1‑130 (MQ=255)                    cTTCTGGGTCGGCTTCTTCACCCTCGCCACTTACGGCAACGCCGGCTGGCTACGCGAACAGGTGTGCGTGTACATGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTg                                                                                                                        <  4:26651/149‑1 (MQ=255)                                       aCCCTCGCCACTTACGGCAACGCCGGCTGGCTACGCGAACAGGTGTGCGTGTACATGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCcgc                                                                                                      <  6:181569/148‑1 (MQ=255)                                                         aaCGCCGGCTGGCTACGCGAACAGGTGTGCGTGTACATGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTa                                                                                   >  4:1414/1‑149 (MQ=255)                                                          aCGCCGGCTGGCTACGCGAACAGGTGTGCGTGTACATGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTa                                                                                   >  6:89784/1‑148 (MQ=255)                                                            gCCGGCTGGCTACACGAACAGGTGTGCGTGTACATGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCcgc                                                                                                      <  3:216664/127‑1 (MQ=255)                                                            gCCGGCTGGCTACACGAACAGGTGTGCGTGTACATGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCcgc                                                                                                      >  4:216664/1‑127 (MQ=255)                                                             ccGGCTGGCTACGCGAACAGGTGTGCGTGTACATGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTTCCGACAAGGACACGATCAGCGTGTCCttgt                                                                                                                                    <  1:21230/97‑4 (MQ=25)                                                             ccGGCTGGCTACGCGAACAGGTGTGCGTGTACATGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGATCAGCGTGTCCttgt                                                                                                                                    >  2:21230/1‑93 (MQ=25)                                                                         gcgAACAGGTGTGCGTGTACATGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTg                                                                   <  3:153216/149‑1 (MQ=255)                                                                          cgAACAGGTGTGCGTGTACATGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTgg                                                                  <  5:89784/149‑1 (MQ=255)                                                                           gAACAGGTGTGCGTGTACATGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTggg                                                                 >  4:219687/1‑149 (MQ=255)                                                                                        tgtACATGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTg                                                                   >  5:261942/1‑134 (MQ=255)                                                                                        tgtACATGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTg                                                                   <  6:261942/134‑1 (MQ=255)                                                                                          tACATGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCATCGACTg                                                  <  3:1414/149‑1 (MQ=255)                                                                                             aTGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCATCGACTGCAc                                               >  5:52729/1‑149 (MQ=255)                                                                                                  ccccTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCGTCGACTGCACCAtgt                                          >  8:142627/1‑149 (MQ=255)                                                                                                  ccccTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCATCGACTGCACCAtgt                                          <  3:219687/149‑1 (MQ=255)                                                                                                     cTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATAc                                                                                         <  5:6693/99‑1 (MQ=255)                                                                                                     cTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATAc                                                                                         >  6:6693/1‑99 (MQ=255)                                                                                                               ttCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCATCGACt                                                   >  8:121218/1‑127 (MQ=255)                                                                                                               ttCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCATCGACt                                                   <  7:121218/127‑1 (MQ=255)                                                                                                                            tGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCATCGACTGCACCATGTGCGTGCAGGTCTGCCCGACCGGCATc                <  6:52729/149‑1 (MQ=255)                                                                                                                                  gACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCATCGACTGCACCATGTGCGTGCAGGt                                <  7:98074/128‑1 (MQ=255)                                                                                                                                  gACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCATCGACTGCACCATGTGCGTGCAGGt                                >  8:98074/1‑128 (MQ=255)                                                                                                                                  gACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCATCGACTGCACCATGTGCGTGCAGGTCTGCCCGACCGGCATCGACAt           >  5:221642/1‑149 (MQ=255)                                                                                                                                    cAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCATCGACTGCACCATGTGCGTGCAGGTCTGCCCGACCGGCATCGACATcc         <  3:67185/149‑1 (MQ=255)                                                                                                                                     taGGACACGATCAGCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCATCGACTGCACCATGTGCGTGCAg                                  >  7:272946/2‑123 (MQ=255)                                                                                                                                     taGGACACGATCAGCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCATCGACTGCACCATGTGCGTGCAg                                  <  8:272946/122‑1 (MQ=255)                                                                                                                                       ggACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCATCGACTGCAGCATGTGCGTGCAGGTCTGCCCGACCGGCATCAACATCCGt       >  5:308480/1‑148 (MQ=255)                                                                                                                                       ggACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCATCGACTGCACCATGTGCGTGCAGGTCTGCCCGACCGGCATCGACATCCGt       >  7:135184/1‑148 (MQ=255)                                                                                                                                       ggACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCATCGACTGCACCATGTGCGTGCAGGTCTGCCCGACCGGCATCGACATCCGTg      >  6:187923/1‑149 (MQ=255)                                                                                                                                           acGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCATCGACTGCACCATGTGCGTGCAGGTCTGCCCGACCGGCATCGACATCCGTGACgg  >  1:170374/1‑149 (MQ=255)                                                                                                                                             gCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCATCGACTGCACCATGTGCGTGCAGGTCTGCCCGAcc                      >  6:259557/1‑127 (MQ=255)                                                                                                                                             gCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCATCGACTGCACCATGTGCGTGCAGGTCTGCCCGAcc                      <  5:259557/127‑1 (MQ=255)                                                                                                                                             gCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCATCGACTGCACCATGTGCGTGCAGGTCTGCCCGACCGGCATc                <  6:315376/133‑1 (MQ=255)                                                                                                                                             gCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCATCGACTGCACCATGTGCGTGCAGGTCTGCCCGACCGGCATc                >  5:315376/1‑133 (MQ=255)                                                                                                                                                |                                                                                                                                                AGGCCGATGGCTGGGCCTACTTCTGGGTCGGCTTCTTCACCCTCGCCACTTACGGCAACGCCGGCTGGCTACGCGAACAGGTGTGCGTGTACATGTGCCCCTATGCCCGCTTCCAGAGCGTGATGTT‑CGACAAGGACACGCTGATCGTGTCCTACGACCCACGCCGTGGCGAAACCCGCGGCCCGCGCAAGAAAGATACCGACTACAAGGCCCAGGGCCTGGGTGACTGCATCGACTGCACCATGTGCGTGCAGGTCTGCCCGACCGGCATCGACATCCGTGACGG  >  NC_002947/4843733‑4844018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑