Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,860,809	G→T	6.0%	L81M (CTG→ATG)	ligA ←	DNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,860,809	0	G	T	6.0%	214.2 / 3.1	67	L81M (CTG→ATG)	ligA	DNA ligase
Reads supporting (aligned to +/- strand):  ref base G (31/32);  new base T (2/2);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

CCAGCTGGCCGTCGCGGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCGG  >  NC_002947/4860662‑4860955                                                                                                                                                    |                                                                                                                                                    ccAGCTGGCCGTCGCGGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAgg                                                                                                                                                    >  8:56951/1‑149 (MQ=255)  cAGCTGGCCGTCGCGGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGc                                                                                                                                                   >  1:19015/1‑149 (MQ=255)     cTGGCCGTCGCGGTACAGCAGGCTCCAGGCCAGGCCATCGAGCTTGGGGTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACTCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGa                                                                                                                                                <  4:225576/149‑1 (MQ=255)                ggTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCATGCTCAGCCTGGGCaac                                                                                                                                     <  6:228589/149‑3 (MQ=255)                 gTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACtt                                                                                                                                    >  5:60961/1‑149 (MQ=255)                    cagcagGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGt                                                                                                                                 <  1:14730/149‑1 (MQ=255)                      gcagGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTgg                                                                                                                               >  2:4652/1‑149 (MQ=255)                            tCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTg                                                                                                                                >  1:154287/1‑142 (MQ=255)                            tCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTg                                                                                                                                <  2:154287/142‑1 (MQ=255)                            tCACGGCCAGGCCATCGAGCTTGGGTTCGCAACTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTAcc                                                                                                                         >  1:220862/1‑149 (MQ=255)                              aCGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCt                                                                                                                        <  2:100239/148‑1 (MQ=255)                               cGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTgg                                                                                                                      <  4:184584/149‑1 (MQ=255)                                ggccaggccaTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGc                                                                                                                     <  6:295583/149‑1 (MQ=255)                                      gccaTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAAcg                                                                                                               >  6:192701/1‑149 (MQ=255)                                         aTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAAcgccg                                                                                                            <  4:133627/149‑1 (MQ=255)                                             aGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACgccgcc                                                                                                          >  7:338410/1‑147 (MQ=255)                                              gCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAg                                                                                                       >  5:233030/1‑149 (MQ=255)                                               cTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAgg                                                                                                      <  6:60961/149‑1 (MQ=255)                                                  gggTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGc                                                                                                     <  2:19015/147‑1 (MQ=255)                                                          aGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCa                                                                                                                                       >  6:160960/1‑105 (MQ=255)                                                          aGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCa                                                                                                                                       <  5:160960/105‑1 (MQ=255)                                                               tAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACg                                                                                     >  8:139160/1‑149 (MQ=255)                                                                  tCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCtg                                                                                  >  1:138564/1‑149 (MQ=255)                                                                  tCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCtg                                                                                  >  1:133274/1‑149 (MQ=255)                                                                    gACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGc                                                                                                     <  1:356415/129‑1 (MQ=255)                                                                    gACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGc                                                                                                     >  2:356415/1‑129 (MQ=255)                                                                    gACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCtgg                                                                                 <  5:192701/148‑1 (MQ=255)                                                                      ccGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCtgggtg                                                                              <  7:139160/149‑1 (MQ=255)                                                                           cccGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGa                                                                         <  1:358284/149‑1 (MQ=255)                                                                              ggCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTc                                                                      <  6:114079/149‑1 (MQ=255)                                                                                  gATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCgggg                                                                  >  2:282316/1‑149 (MQ=255)                                                                                   aTCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCggggg                                                                 >  4:82676/1‑149 (MQ=255)                                                                                   aTCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCgggg                                                                  <  4:118478/148‑1 (MQ=255)                                                                                      gAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCtgggtggg                                                                            <  2:309663/135‑1 (MQ=255)                                                                                      gAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACATCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCtgggtggg                                                                            >  1:309663/1‑135 (MQ=255)                                                                                      gAGGCCTTCTACCACGCGACGGCCCAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTa                                                              >  6:155411/1‑149 (MQ=255)                                                                                       aGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTAc                                                             >  3:287764/1‑149 (MQ=255)                                                                                           cTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAgg                                                         >  4:142028/1‑149 (MQ=255)                                                                                           cTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAgg                                                         >  2:175746/1‑149 (MQ=255)                                                                                                  cACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTccac                                                                                            <  6:344111/107‑1 (MQ=255)                                                                                                  cACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTccac                                                                                            >  5:344111/1‑107 (MQ=255)                                                                                                      cgACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTc                                              <  4:287764/149‑1 (MQ=255)                                                                                                       gACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCgg                                                    >  6:325185/1‑142 (MQ=255)                                                                                                       gACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCgg                                                    <  5:325185/142‑1 (MQ=255)                                                                                                          ggCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTg                                                                                                                   >  7:160573/1‑77 (MQ=255)                                                                                                          ggCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTg                                                                                                                   <  8:160573/77‑1 (MQ=255)                                                                                                              gAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTc                                        >  2:272669/1‑147 (MQ=255)                                                                                                              gAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCa                                      <  7:56951/149‑1 (MQ=255)                                                                                                               aaCTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAg                                     >  2:350242/1‑149 (MQ=255)                                                                                                               aaCTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAg                                     <  6:233030/149‑1 (MQ=255)                                                                                                                      gCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCtt                               >  1:169742/1‑148 (MQ=255)                                                                                                                             ggCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGt                       <  2:169742/149‑1 (MQ=255)                                                                                                                                  cttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGaa                   >  4:95041/1‑148 (MQ=255)                                                                                                                                  cttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGaa                   >  4:260022/1‑148 (MQ=255)                                                                                                                                  cttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGa                    <  3:82676/147‑1 (MQ=255)                                                                                                                                  cttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGa                    <  1:350242/147‑1 (MQ=255)                                                                                                                                   ttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGtt                      >  1:7403/1‑144 (MQ=255)                                                                                                                                   ttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGtt                      <  2:7403/144‑1 (MQ=255)                                                                                                                                     cGAAGGCGTTGCCCAGGCTGAGCATGGGCACTGCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCATCGCCTTGAGCTCGTTGAACAg                <  2:220862/148‑1 (MQ=255)                                                                                                                                      gAAGGCGTTGCCCAGGCTGAGCATGGGCGCTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGc              >  6:117713/1‑149 (MQ=255)                                                                                                                                       cacgaagtGCCCATGCTCAGCCTGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCg             >  5:97540/8‑149 (MQ=255)                                                                                                                                       aaGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCg             <  1:282316/149‑1 (MQ=255)                                                                                                                                          gCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGTGCTCGTTGAACAGGCGGTc          <  5:155411/149‑1 (MQ=255)                                                                                                                                             gtGCCCATGCTCAGCCTGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGt                       <  6:162539/132‑1 (MQ=255)                                                                                                                                             gtGCCCATGCTCAGCCTGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTCAGCTCGt                       >  5:162539/2‑133 (MQ=255)                                                                                                                                                 ccAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGACTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTc    >  1:274366/1‑149 (MQ=255)                                                                                                                                                 ccAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCg   <  1:76487/149‑1 (MQ=255)                                                                                                                                                  cAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCgg  <  3:95041/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CCAGCTGGCCGTCGCGGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCGG  >  NC_002947/4860662‑4860955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑