Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,860,817	A→C	6.2%	M78R (ATG→AGG)	ligA ←	DNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,860,817	0	A	C	6.2%	202.1 / 4.7	65	M78R (ATG→AGG)	ligA	DNA ligase
Reads supporting (aligned to +/- strand):  ref base A (30/31);  new base C (2/2);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.97e-01

GGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCGG  >  NC_002947/4860677‑4860955                                                                                                                                             |                                                                                                                                            ggTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCATGCTCAGCCTGGGCaac                                                                                                                                     <  6:228589/149‑3 (MQ=255)  gTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACtt                                                                                                                                    >  5:60961/1‑149 (MQ=255)     cagcagGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGt                                                                                                                                 <  1:14730/149‑1 (MQ=255)       gcagGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTgg                                                                                                                               >  2:4652/1‑149 (MQ=255)             tCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTg                                                                                                                                >  1:154287/1‑142 (MQ=255)             tCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTg                                                                                                                                <  2:154287/142‑1 (MQ=255)             tCACGGCCAGGCCATCGAGCTTGGGTTCGCAACTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTAcc                                                                                                                         >  1:220862/1‑149 (MQ=255)               aCGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCt                                                                                                                        <  2:100239/148‑1 (MQ=255)                cGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTgg                                                                                                                      <  4:184584/149‑1 (MQ=255)                 ggccaggccaTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGc                                                                                                                     <  6:295583/149‑1 (MQ=255)                       gccaTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAAcg                                                                                                               >  6:192701/1‑149 (MQ=255)                          aTCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAAcgccg                                                                                                            <  4:133627/149‑1 (MQ=255)                              aGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACgccgcc                                                                                                          >  7:338410/1‑147 (MQ=255)                               gCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAg                                                                                                       >  5:233030/1‑149 (MQ=255)                                cTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAgg                                                                                                      <  6:60961/149‑1 (MQ=255)                                   gggTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGc                                                                                                     <  2:19015/147‑1 (MQ=255)                                           aGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCa                                                                                                                                       <  5:160960/105‑1 (MQ=255)                                           aGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCa                                                                                                                                       >  6:160960/1‑105 (MQ=255)                                                tAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACg                                                                                     >  8:139160/1‑149 (MQ=255)                                                   tCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCtg                                                                                  >  1:133274/1‑149 (MQ=255)                                                   tCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCtg                                                                                  >  1:138564/1‑149 (MQ=255)                                                     gACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGc                                                                                                     >  2:356415/1‑129 (MQ=255)                                                     gACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGc                                                                                                     <  1:356415/129‑1 (MQ=255)                                                     gACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCtgg                                                                                 <  5:192701/148‑1 (MQ=255)                                                       ccGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCtgggtg                                                                              <  7:139160/149‑1 (MQ=255)                                                            cccGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGa                                                                         <  1:358284/149‑1 (MQ=255)                                                               ggCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTc                                                                      <  6:114079/149‑1 (MQ=255)                                                                   gATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCgggg                                                                  >  2:282316/1‑149 (MQ=255)                                                                    aTCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCggggg                                                                 >  4:82676/1‑149 (MQ=255)                                                                    aTCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCgggg                                                                  <  4:118478/148‑1 (MQ=255)                                                                       gAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCtgggtggg                                                                            <  2:309663/135‑1 (MQ=255)                                                                       gAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACATCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCtgggtggg                                                                            >  1:309663/1‑135 (MQ=255)                                                                       gAGGCCTTCTACCACGCGACGGCCCAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTa                                                              >  6:155411/1‑149 (MQ=255)                                                                        aGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTAc                                                             >  3:287764/1‑149 (MQ=255)                                                                            cTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAgg                                                         >  4:142028/1‑149 (MQ=255)                                                                            cTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAgg                                                         >  2:175746/1‑149 (MQ=255)                                                                                   cACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTccac                                                                                            <  6:344111/107‑1 (MQ=255)                                                                                   cACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTccac                                                                                            >  5:344111/1‑107 (MQ=255)                                                                                       cgACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTc                                              <  4:287764/149‑1 (MQ=255)                                                                                        gACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCgg                                                    >  6:325185/1‑142 (MQ=255)                                                                                        gACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCgg                                                    <  5:325185/142‑1 (MQ=255)                                                                                           ggCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTg                                                                                                                   <  8:160573/77‑1 (MQ=255)                                                                                           ggCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTg                                                                                                                   >  7:160573/1‑77 (MQ=255)                                                                                               gAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTc                                        >  2:272669/1‑147 (MQ=255)                                                                                               gAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCa                                      <  7:56951/149‑1 (MQ=255)                                                                                                aaCTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAg                                     >  2:350242/1‑149 (MQ=255)                                                                                                aaCTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAg                                     <  6:233030/149‑1 (MQ=255)                                                                                                       gCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCtt                               >  1:169742/1‑148 (MQ=255)                                                                                                              ggCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGt                       <  2:169742/149‑1 (MQ=255)                                                                                                                   cttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGaa                   >  4:260022/1‑148 (MQ=255)                                                                                                                   cttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGaa                   >  4:95041/1‑148 (MQ=255)                                                                                                                   cttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGa                    <  1:350242/147‑1 (MQ=255)                                                                                                                   cttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGa                    <  3:82676/147‑1 (MQ=255)                                                                                                                    ttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGtt                      >  1:7403/1‑144 (MQ=255)                                                                                                                    ttcGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGtt                      <  2:7403/144‑1 (MQ=255)                                                                                                                      cGAAGGCGTTGCCCAGGCTGAGCATGGGCACTGCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCATCGCCTTGAGCTCGTTGAACAg                <  2:220862/148‑1 (MQ=255)                                                                                                                       gAAGGCGTTGCCCAGGCTGAGCATGGGCGCTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGc              >  6:117713/1‑149 (MQ=255)                                                                                                                        cacgaagtGCCCATGCTCAGCCTGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCg             >  5:97540/8‑149 (MQ=255)                                                                                                                        aaGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCg             <  1:282316/149‑1 (MQ=255)                                                                                                                           gCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGTGCTCGTTGAACAGGCGGTc          <  5:155411/149‑1 (MQ=255)                                                                                                                              gtGCCCATGCTCAGCCTGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGt                       <  6:162539/132‑1 (MQ=255)                                                                                                                              gtGCCCATGCTCAGCCTGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTCAGCTCGt                       >  5:162539/2‑133 (MQ=255)                                                                                                                                  ccAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAGACTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTc    >  1:274366/1‑149 (MQ=255)                                                                                                                                  ccAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCg   <  1:76487/149‑1 (MQ=255)                                                                                                                                   cAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCgg  <  3:95041/149‑1 (MQ=255)                                                                                                                                             |                                                                                                                                            GGTACAGCAGGCTCACGGCCAGGCCATCGAGCTTGGGTTCGCAGCTGTAGTCGACCGCGCCCGGCTGATCGAGGCCTTCTACCACGCGACGGCCGAACTCACGCAGGTCGGCTTCTTCGAAGGCGTTGCCCAGGCTGAGCATGGGCACTTCGTGGCGTACCTGGCTGAACGCCGCCAAGGCAG‑CTCCACCCACACGCTGGGTGGGCGAGTCGGGGGTTACCAGGTGCGGGTGTTCAGCTTCCAGCGCCTTGAGCTCGTTGAACAGGCGGTCGTATTCGG  >  NC_002947/4860677‑4860955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑