| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,896,377 | C→T | 9.5% | intergenic (+10/‑198) | PP_4304 → / → sbp‑I | VIC family cation transporter/sulfate ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,896,377 | 0 | C | T | 9.5% | 111.7 / 2.8 | 43 | intergenic (+10/‑198) | PP_4304/sbp‑I | VIC family cation transporter/sulfate ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (18/20); new base T (2/2); total (20/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
TTTACCGCCGAGCTGGCCAATGCCATGCGTGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAAC > NC_002947/4896233‑4896506 | tttACCGCCGAGCTGGCCAATGCCATGCGTGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCG > 4:183366‑M1/1‑149 (MQ=255) ttACCGCCGAGCTGGCCAATGCCATGCGTGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACCAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAAGGGGTTGTTGACCAAAGCGTGATAGCGACTGTGCCGG > 8:318882‑M1/1‑149 (MQ=255) cTGGCCAATGCCATGCGTGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGTGTGATAGCGCCTGCGCCGGCCTCTTCGCGG < 1:214128‑M1/149‑1 (MQ=255) ttgccAATGCGATGCGTGGGGAGCAGCTGCAGCTTGATTGCCCGACCTGCCACAAGAAGACCCAGGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGATTGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGG < 5:92930‑M1/147‑1 (MQ=255) cAATGCCATGCGTGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAA > 4:153107‑M1/1‑149 (MQ=255) aTGCCATGCGTGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATc < 3:183366/149‑1 (MQ=255) ggAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGgc > 1:236435/1‑149 (MQ=255) gAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGgcg > 6:223639/1‑149 (MQ=255) aTGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGcgcg < 5:223639/149‑1 (MQ=255) aTGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCCGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGcgcg < 5:76412/149‑1 (MQ=255) ccGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGTGCCGGCCTCTTCGCGGGTAAAcccgctcctacgggggaccgcgcacggtccgcgcctg > 8:66285‑M1/1‑112 (MQ=255) cGACCTGCCACAAGAAGACCCCCGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAAGGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGt < 3:14540/149‑1 (MQ=255) aCCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAg < 3:153107/149‑1 (MQ=255) ccACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAAcccgctcctacgggggaccgcgcacggtccgcgcctgtaggagcg > 7:206633‑M1/1‑104 (MQ=255) acaAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAAtccgctcctacgggggaccgcgcacggtccgcgcctgtagg < 3:240857‑M1/143‑42 (MQ=255) acaAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAAtccgctcctacgggggaccgcgcacggtccgcgcctgtagg > 4:240857‑M1/1‑102 (MQ=255) acaAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCggg > 7:290130/1‑149 (MQ=255) acaAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGCGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCACTTAGCCGGTAAATCCGCACCTACACCCGCGGACCCAGCGCCGGCCCACCTAGCAGCcgg > 5:310614/1‑149 (MQ=255) caAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGTGCCGGCCTCTTCGCGGGTAAAcccgctcctacgggggaccgcgcacggtccgcgcctgtaggagcggat < 7:318882‑M1/149‑49 (MQ=255) aagaagACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGTGCCGGCCTCTTCGCGGGTAAAcccgctcctacgggggaccgcgcacggtccgcgcctgtaggagcggatt < 7:66285‑M1/149‑50 (MQ=255) gaagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAAcccgctcctacgggggaccgcgcacggtccgcgcctgtaggagcggattta < 8:206633‑M1/149‑52 (MQ=255) gaagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATcc > 5:363727/1‑101 (MQ=255) gaagaCCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATcc < 6:363727/101‑1 (MQ=255) ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAGAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAAcccgctcctacgggggaccgcgcacggtccgcgcctgtaggagcggat > 1:118247‑M1/1‑92 (MQ=255) ccACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAGAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAAcccgctcctacgggggaccgcgcacggtccgcgcctgtaggagcggat < 2:118247‑M1/140‑49 (MQ=255) cccgcgCAGGGGGCGGCGTTCTGTTCTCGCTGTGGTAATGGGTTGTTTCCCAACGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGTTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTCGTAGCGGGTTTACCCGCGa < 8:290130/144‑1 (MQ=255) gCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGtttacccgcgaagagg < 1:215820‑M2/149‑17 (MQ=255) gttctgttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGtttacccgcgaagaggccg < 6:256850‑M2/140‑20 (MQ=255) gttctgttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGtttacccgcgaagaggccg > 5:256850‑M2/1‑121 (MQ=255) ttctgttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCCCGGGTAAATCCGCGCCTACAGGCGCGGACCGTGCGCGGTCCCCCGGAGGAGCGGGtttacccgcgaagaggccgtgacagg < 8:157163‑M2/146‑27 (MQ=255) tgttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAg > 6:264293/1‑149 (MQ=255) ttctCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAg < 2:215820/147‑1 (MQ=255) ggtgtgGGAATGGGTGTTTTCAGAAAGCGTTATAGCGCGTGGGGCGGCGTCTTCGCGGGTAATTGCGCTGGTACTGGCGGGGAGCGTGGGCGGTCCCCGGTTGGAGCGGGTTTACCGGCTATGAGGCCGGCACAGGCAGAAGCATAAc < 6:310614/146‑1 (MQ=255) gCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGAAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATaa < 7:157163/147‑1 (MQ=255) ggAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTTAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGGAGGAGCGGGTTTAACCGCGAAGCGGCCGGCACCGGCAGAAGCATAACCAAAGcc > 3:328453/1‑148 (MQ=255) aaTGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCATCGCGGGTTAATCCGCTCCTACAGGCGCGGCCCGTGGGCGGTCCCCCGTTGGAGCGGGTTTTCCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCAtt > 1:122271/1‑149 (MQ=39) gttgttTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGCGCGGGTTTACCCGCGCAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTg > 5:260402/1‑149 (MQ=255) gttgttTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCCCGGGTAAATCCGCTCCTACAGGCGCCGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGCACAGGCAGAAGCATAACCAAAGCATTTTTTg > 6:103088/1‑149 (MQ=255) ttgttTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATACGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGt > 8:166621/1‑149 (MQ=255) aattCCCACAGCGTTATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCAACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGTTTTACCGGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTc < 5:315431/147‑1 (MQ=38) cAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAAc < 5:264293/149‑1 (MQ=255) aaaGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTccccc > 3:133932/1‑76 (MQ=255) aaaGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTccccc < 4:133932/76‑1 (MQ=255) | TTTACCGCCGAGCTGGCCAATGCCATGCGTGGGGAGCAGCTGCAGCATGATTGCCCGACCTGCCACAAGAAGACCCACGAGCAGGGGGCGGCGTTCTGTTCTCGCTGTGGGAATGGGTTGTTTCCCAAAGCGTGATAGCGCCTGCGCCGGCCTCTTCGCGGGTAAATCCGCTCCTACAGGCGCGGACCGTGCGCGGTCCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAGAAGCATAACCAAAGCATTTTTTGTTCTTTAAC > NC_002947/4896233‑4896506 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |