Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 356,599 G→A 7.8% intergenic (‑396/‑57) cbcX ← / → tdcG‑I choline/betaine/carnitine ABC transporter substrate‑binding protein/L‑serine dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947356,5990GA 7.8% 145.7 / 2.1 51intergenic (‑396/‑57)cbcX/tdcG‑Icholine/betaine/carnitine ABC transporter substrate‑binding protein/L‑serine dehydratase
Reads supporting (aligned to +/- strand):  ref base G (22/25);  new base A (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

GAAAACGGCGCGGTGGGCGCAGTTTCTGGTTGAGAACAGCGCGGCGCTGCTGGACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGTTCGACCTGTTCAAGATCGGTGTCGGGCCCTCCAGCTCCCACACCGTCGGCCCCATGCGTGCTGGCGCCCTGTT  >  NC_002947/356457‑356744
                                                                                                                                              |                                                                                                                                                 
gAAAACGGCGCGGTGGGCGCAGTTTCTGGTTGAGAACAGCGCGGCGCTGCTGGACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTAccc                                                                                                                                             >  4:31886/1‑149 (MQ=255)
gAAAACGGCGCGGTGGGCGCAGTTTCTGGTTGAGAACAGCGCGGCGCTGCTGGACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTAc                                                                                                                                               <  5:212432/147‑1 (MQ=255)
   aaCGGCGCGGTGGGCGCAGTTTCTGGTTGAGAACAGCGCGGCGCTGCTGGACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGc                                                                                                                                          >  4:227781/1‑149 (MQ=255)
      ggCGCGGTGGGCGCAGTTTCTGGTTGAGAACAGCGCGGCGCTGCTGGACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCt                                                                                                                                       <  7:49875/149‑1 (MQ=255)
            gTGGGCGCAGTTTCTGGTTGAGAACAGCGCGGCGCTGCTGGACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTAc                                                                                                                                               <  8:20384/135‑1 (MQ=255)
            gTGGGCGCAGTTTCTGGTTGAGAACAGCGCGGCGCTGCTGGACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTAc                                                                                                                                               >  7:20384/1‑135 (MQ=255)
                      tttCTGGTTGAGAACAGCGCGGCGCTGCTGGACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCaga                                                                                                                       <  1:8063/149‑1 (MQ=255)
                           ggTTGAGAACAGCGCGGCGCTGCTGGACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGAc                                                                                                                    >  2:336389/1‑147 (MQ=255)
                            gTTGAGAACAGCGCGGCGCTGCTGGACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGAc                                                                                                                    <  3:38968/146‑1 (MQ=255)
                            gTTGAGAACAGCGCGGCGCTGCTGGACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAg                                                                                                                 <  1:336389/149‑1 (MQ=255)
                            gTTGAGAACAGCGCGGCGCTGCTGGACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAg                                                                                                                 >  7:127701/1‑149 (MQ=255)
                                 gaACAGCGCGGCGCTGCTGGACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGTCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCCGCTACCCCGCCCTGCGCATGGCGCGCAGAGCCATGCCCAg                                                                                                            >  8:154055/1‑149 (MQ=255)
                                 gaACAGCGCGGCGCTGCTGGACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGACCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAg                                                                                                            >  4:8484/1‑149 (MQ=255)
                                      gcgcGGCGCTGCTGGACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAaga                                                                                                       <  5:296054/149‑1 (MQ=255)
                                             gctgctTGACAAAAGTACGTCGTTTGCAGACGGCGAGGTTGTCGGTAAAATACCGATGATGCTTGCATTCAAGGCTGCCAGCTTTTTGAGACTAGCAGCTACCCCGCCCTGCGAATGGCTCGCAGAGACAAGCCCAGCAAGATGTTAtt                                                                                                <  7:154055/149‑1 (MQ=255)
                                             gctgctGGACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGAtt                                                                                                <  3:8484/149‑1 (MQ=255)
                                                 ctgGACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGa                                                                                             >  4:13556/1‑148 (MQ=255)
                                                 ctgGACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAACTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGa                                                                                             >  8:174564/1‑148 (MQ=255)
                                                 ctgGACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAACTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGa                                                                                             >  8:179596/1‑148 (MQ=255)
                                                    gACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGGCCAGCAAGAGGTGAt                                                                                                 <  5:64218/141‑1 (MQ=255)
                                                    gACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAAt                                                                                         >  6:294571/1‑149 (MQ=255)
                                                    gACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGATGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGAt                                                                                                 >  6:64218/1‑141 (MQ=255)
                                                     aCAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCaga                                                                                                                       <  4:45350/118‑1 (MQ=255)
                                                     aCAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAagag                                                                                                      >  3:42492/1‑135 (MQ=255)
                                                     aCAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAagag                                                                                                      <  4:42492/135‑1 (MQ=255)
                                                        aaaGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGa                                                                                             >  3:72507/1‑141 (MQ=255)
                                                        aaaGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGa                                                                                             <  4:72507/141‑1 (MQ=255)
                                                              cGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCa                                                                                 <  3:13556/147‑1 (MQ=255)
                                                                     tGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGTTc                                                                         >  8:47709/1‑148 (MQ=255)
                                                                        aGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGTTCGAc                                                                      <  7:134202/148‑1 (MQ=255)
                                                                             gCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGTTCGACCTGtt                                                                 >  6:359754/1‑148 (MQ=255)
                                                                              cGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGa                                                                                                  >  1:301125/1‑114 (MQ=255)
                                                                              cGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGa                                                                                                  <  2:301125/114‑1 (MQ=255)
                                                                               gAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGTTCGACCTGTTCaa                                                              <  7:47709/149‑1 (MQ=255)
                                                                                       cGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGTTCGACCTGTTCAAGATCGgtg                                                      >  6:104812/1‑149 (MQ=255)
                                                                                       cGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGTTCGACCTGTTCAAGATCGgtg                                                      >  1:354026/1‑149 (MQ=255)
                                                                                            aaaGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAACTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGTTCGACCTGTTCAAGATCGGTGTCggg                                                 <  7:174564/149‑1 (MQ=255)
                                                                                            aaaGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAACTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGTTCGACCTGTTCAAGATCGGTGTCggg                                                 <  7:179596/149‑1 (MQ=255)
                                                                                                  cGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGCCTAGCCCAGCAAGAGGTGATTCGACAATGGCAATCAGCCTGTTCGACCTGTTCAACATCGGTGTCGGGCCCTcc                                           >  8:327558/1‑149 (MQ=255)
                                                                                                  cGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGTTCGACCTGTTCAAGATCGGTGTCGGGCCCTcc                                           <  5:359754/149‑1 (MQ=255)
                                                                                                        tgCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGTTCGACCTGTTCAAGATCGGTGTCGGGCCCTCCAGCTcc                                     <  5:294571/149‑1 (MQ=255)
                                                                                                         gCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGTTCGACCTGTTCAAGATCGGTGTCGGGCCCTCCAGCTcc                                     <  3:31886/148‑1 (MQ=255)
                                                                                                          cTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCAGGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGTTCGACCTGTTCAAGATCGGTGTCGGGCCCTCCAGCTccc                                    <  7:327558/148‑1 (MQ=255)
                                                                                                              cATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGTTCGACCTGTTCAAGATCGGTGTCGGGCCCTCCAGCTCCCACAcc                               <  1:301288/149‑1 (MQ=255)
                                                                                                                       cggcCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGTTCGACCTGTTCAAGATCGGTGTCGGGCCCTCCAGCTCCCACACCGTCGGcccc                      <  3:227781/149‑1 (MQ=255)
                                                                                                                         gcCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGTTCGACCTGTTCAAGATCGGTGTCGGGCCCTCCAGCTCCCACACCGTCGGCCCCAt                    <  5:104812/149‑1 (MQ=255)
                                                                                                                            cGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGtt                                                                          <  2:184062/92‑1 (MQ=255)
                                                                                                                            cGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGtt                                                                          >  1:184062/1‑92 (MQ=255)
                                                                                                                                 tttGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGTTCGACCTGTTCAAGATCGGTGTCGGGCCCTCCAGCTCCCACACCGTCGGCCCCATGCGTGCTg            >  2:65499/1‑149 (MQ=255)
                                                                                                                                         tAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGTTCGACCTGTTCAAGATCGGTGTCGGGCCCTCCAGCTCCCACACCGTCGGCCCCATGCGTGCTGGCGCCCTg    >  6:357818/1‑149 (MQ=255)
                                                                                                                                         tAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGTTCGACCTGTTAAAGATCGGTGTCGGGCCCTCCAGCTCCCACACCGTCGGCCCCATGCGTGCTGGCGCCCTg    >  4:298119/1‑149 (MQ=255)
                                                                                                                                           gcagcTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGTTCGACCTGTTCAAGATCGGTGTCGGGCCCTCCAGCTCCCACACCGTCGGCCCCATGCGTGCTGGCGCCCTgtt  <  8:127701/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
GAAAACGGCGCGGTGGGCGCAGTTTCTGGTTGAGAACAGCGCGGCGCTGCTGGACAAAAGTACGTCGGTTGCAGACGGCGAGGTGGGCGGTAAAAGCCCGATGATGCTGGCATTCAAGGCGGCCCGCTGTTTGAGCCTAGCAGCTACCCCGCCCTGCGCATGGCGCGCAGAGACAAGCCCAGCAAGAGGTGATTCGACAATGGCCATCAGCGTGTTCGACCTGTTCAAGATCGGTGTCGGGCCCTCCAGCTCCCACACCGTCGGCCCCATGCGTGCTGGCGCCCTGTT  >  NC_002947/356457‑356744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑