Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,970,603	T→C	11.1%	intergenic (‑361/+33)	fliC ← / ← PP_4379	flagellin/3‑oxoacyl‑ACP synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,970,603	0	T	C	11.1%	127.9 / 7.8	54	intergenic (‑361/+33)	fliC/PP_4379	flagellin/3‑oxoacyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base T (23/25);  new base C (3/3);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.57e-01

TCATCCCGTCGGTGGAGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAACACTG  >  NC_002947/4970455‑4970750                                                                                                                                                     |                                                                                                                                                     tCATCCCGTCGGTGGAGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGt                                                                                                                                                      >  2:76431/1‑149 (MQ=255) tCATCCCGTCGGTGGAGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGt                                                                                                                                                      >  2:42940/1‑149 (MQ=255)   aTCCCGTCGGTGGAGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTg                                                                                                                                                     <  8:300458/148‑1 (MQ=255)                aGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCtt                                                                                                                                       <  5:139280/149‑1 (MQ=255)                     gTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGCGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAgg                                                                                                                                  <  3:261297/149‑1 (MQ=255)                       gAGGCTGCTAATACGTACACGCGACGGGTGGAACCGGCTTTGCCGGTGTTCGCGTGTGAACCCGCGCCTACAATGGGCTGCGTTAGGAAGAATCTTATTTTCGGCATAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAggg                                                                                                                                 <  8:115477/148‑1 (MQ=255)                        aGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCCGGCGGCCACCGCCCGGCGCttttttttc                                                                                                                                <  7:266437/148‑6 (MQ=37)                               gaagaaGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTgg                                                                                                                        >  8:268331/1‑149 (MQ=255)                                 agaagGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGTCGGGCGCTTTTAGGGGCTGACTGGGa                                                                                                                      <  1:193374/149‑1 (MQ=255)                                      gagaCGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGt                                                                                                                 >  8:183888/1‑149 (MQ=255)                                          cgcgATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTcgcg                                                                                                             <  7:183888/149‑1 (MQ=255)                                                 gcgaacACCGGCAAAGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGa                                                                                                                      >  1:131661/6‑133 (MQ=39)                                                 gcgaacACCGGCAAAGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGa                                                                                                                      <  2:131661/128‑1 (MQ=39)                                                       aCCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGcc                                                                                                <  7:268331/149‑1 (MQ=255)                                                              ttGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGcccc                                                                                         >  8:66579/1‑149 (MQ=255)                                                                     tgtTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAg                                                                                  <  7:135466/149‑1 (MQ=255)                                                                          gcgGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGa                                                                                                                      >  4:52840/1‑108 (MQ=255)                                                                          gcgGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGa                                                                                                                      <  3:52840/108‑1 (MQ=255)                                                                                   cccGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGc                                                                    >  7:39845/1‑149 (MQ=255)                                                                                      gCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCAc                                                                 >  2:9271/1‑149 (MQ=255)                                                                                          cTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTcgcg                                                                                                             <  6:348532/101‑1 (MQ=255)                                                                                           tACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATc                                                            >  8:321708/1‑149 (MQ=255)                                                                                                tGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCAc                                                       <  4:162996/149‑1 (MQ=255)                                                                                                  ggCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCAcgc                                                     >  2:330512/1‑149 (MQ=255)                                                                                                  ggCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCAcgc                                                     <  7:66579/149‑1 (MQ=255)                                                                                                   gCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCAcgcg                                                    >  6:143761/1‑149 (MQ=255)                                                                                                     tGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCt                                                  >  8:294239/1‑149 (MQ=255)                                                                                                         ttAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTc                                                >  1:114272/1‑147 (MQ=255)                                                                                                             gCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑g                                         >  2:173409/1‑149 (MQ=255)                                                                                                              cAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑Gc                                        >  1:177728/1‑149 (MQ=255)                                                                                                               aGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑Gc                                        >  7:221598/1‑148 (MQ=255)                                                                                                                    cTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATc                                    >  1:89945/1‑147 (MQ=255)                                                                                                                     ttATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑Gcc                                       <  4:184117/143‑1 (MQ=255)                                                                                                                     ttATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑Gcc                                       >  3:184117/1‑143 (MQ=255)                                                                                                                        ttttCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCAt                              >  4:109439/1‑149 (MQ=255)                                                                                                                             ggCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGCGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCt                         >  3:222769/1‑149 (MQ=255)                                                                                                                             ggCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGc                          <  5:224077/148‑1 (MQ=255)                                                                                                                             ggCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGc                          <  1:173409/148‑1 (MQ=255)                                                                                                                              gCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCtt                        <  1:9271/149‑1 (MQ=255)                                                                                                                              gCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCtt                        <  2:89945/149‑1 (MQ=255)                                                                                                                              gCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCt                         >  2:181552/1‑148 (MQ=255)                                                                                                                                aGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCGTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGCGCCCCACGACAGGCCCCCGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCTTCt                      >  5:178098/1‑149 (MQ=255)                                                                                                                                  aaaaaaaaGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTcg                                                                                                               >  6:2063/1‑59 (MQ=255)                                                                                                                                  aaaaaaaaGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTcg                                                                                                               <  5:2063/59‑1 (MQ=255)                                                                                                                                   cctaaaaGCGCCCGGCGGCCACCGCCCGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCTTCTg                     >  6:113735/4‑147 (MQ=39)                                                                                                                                   aaaaaaaGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCg                                   >  2:204700/1‑133 (MQ=255)                                                                                                                                   aaaaaaaGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCg                                   <  1:204700/133‑1 (MQ=255)                                                                                                                                     taaaaGCGCCCGGCGGCCACCGCCCGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCa                                                                                        <  3:182987/78‑1 (MQ=25)                                                                                                                                     taaaaGCGCCCGGCGGCCACCGCCCGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCa                                                                                        >  4:182987/2‑79 (MQ=25)                                                                                                                                        aaGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCTTCTgcagcagc              <  2:114272/149‑1 (MQ=255)                                                                                                                                        aaGCGCCCGGCGGCCACCGCCCGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGc                          <  1:35299/137‑1 (MQ=255)                                                                                                                                        aaGCGCCCGGCGGCCACCGCCCGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGc                          >  2:35299/1‑137 (MQ=255)                                                                                                                                            gcCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCTTCTGCAGCAGCAGCg          <  1:76431/149‑1 (MQ=255)                                                                                                                                             ccGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTAGCCATCGAGCATGTGCTTCTGCAGCAGCAGCg          <  1:42940/149‑1 (MQ=255)                                                                                                                                                  cGGTGGCCGCCGGGCGCTTTTAGGGGCTTACTGGGAGCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCTGCTGCAGCAGCAGCGGAacac    <  6:178098/149‑1 (MQ=255)                                                                                                                                                    gTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAACACTg  <  8:39845/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     TCATCCCGTCGGTGGAGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAACACTG  >  NC_002947/4970455‑4970750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑