Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,970,611 G→C 11.0% intergenic (‑369/+25) fliC ← / ← PP_4379 flagellin/3‑oxoacyl‑ACP synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,970,6110GC11.0% 147.5 / 8.3 54intergenic (‑369/+25)fliC/PP_4379flagellin/3‑oxoacyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base G (23/25);  new base C (3/3);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01

AGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAACACTGGAGGACACC  >  NC_002947/4970470‑4970759
                                                                                                                                             |                                                                                                                                                     
aGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCtt                                                                                                                                                <  5:139280/149‑1 (MQ=255)
     gTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGCGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAgg                                                                                                                                           <  3:261297/149‑1 (MQ=255)
       gAGGCTGCTAATACGTACACGCGACGGGTGGAACCGGCTTTGCCGGTGTTCGCGTGTGAACCCGCGCCTACAATGGGCTGCGTTAGGAAGAATCTTATTTTCGGCATAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAggg                                                                                                                                          <  8:115477/148‑1 (MQ=255)
        aGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCCGGCGGCCACCGCCCGGCGCttttttttc                                                                                                                                         <  7:266437/148‑6 (MQ=37)
               gaagaaGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTgg                                                                                                                                 >  8:268331/1‑149 (MQ=255)
                 agaagGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGTCGGGCGCTTTTAGGGGCTGACTGGGa                                                                                                                               <  1:193374/149‑1 (MQ=255)
                      gagaCGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGt                                                                                                                          >  8:183888/1‑149 (MQ=255)
                          cgcgATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTcgcg                                                                                                                      <  7:183888/149‑1 (MQ=255)
                                 gcgaacACCGGCAAAGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGa                                                                                                                               >  1:131661/6‑133 (MQ=39)
                                 gcgaacACCGGCAAAGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGa                                                                                                                               <  2:131661/128‑1 (MQ=39)
                                       aCCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGcc                                                                                                         <  7:268331/149‑1 (MQ=255)
                                              ttGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGcccc                                                                                                  >  8:66579/1‑149 (MQ=255)
                                                     tgtTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAg                                                                                           <  7:135466/149‑1 (MQ=255)
                                                          gcgGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGa                                                                                                                               >  4:52840/1‑108 (MQ=255)
                                                          gcgGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGa                                                                                                                               <  3:52840/108‑1 (MQ=255)
                                                                   cccGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGc                                                                             >  7:39845/1‑149 (MQ=255)
                                                                      gCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCAc                                                                          >  2:9271/1‑149 (MQ=255)
                                                                          cTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTcgcg                                                                                                                      <  6:348532/101‑1 (MQ=255)
                                                                           tACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATc                                                                     >  8:321708/1‑149 (MQ=255)
                                                                                tGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCAc                                                                <  4:162996/149‑1 (MQ=255)
                                                                                  ggCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCAcgc                                                              >  2:330512/1‑149 (MQ=255)
                                                                                  ggCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCAcgc                                                              <  7:66579/149‑1 (MQ=255)
                                                                                   gCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCAcgcg                                                             >  6:143761/1‑149 (MQ=255)
                                                                                     tGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCt                                                           >  8:294239/1‑149 (MQ=255)
                                                                                         ttAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTc                                                         >  1:114272/1‑147 (MQ=255)
                                                                                             gCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑g                                                  >  2:173409/1‑149 (MQ=255)
                                                                                              cAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑Gc                                                 >  1:177728/1‑149 (MQ=255)
                                                                                               aGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑Gc                                                 >  7:221598/1‑148 (MQ=255)
                                                                                                    cTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATc                                             >  1:89945/1‑147 (MQ=255)
                                                                                                     ttATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑Gcc                                                <  4:184117/143‑1 (MQ=255)
                                                                                                     ttATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑Gcc                                                >  3:184117/1‑143 (MQ=255)
                                                                                                        ttttCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCAt                                       >  4:109439/1‑149 (MQ=255)
                                                                                                             ggCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGCGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCt                                  >  3:222769/1‑149 (MQ=255)
                                                                                                             ggCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGc                                   <  1:173409/148‑1 (MQ=255)
                                                                                                             ggCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGc                                   <  5:224077/148‑1 (MQ=255)
                                                                                                              gCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCtt                                 <  1:9271/149‑1 (MQ=255)
                                                                                                              gCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCtt                                 <  2:89945/149‑1 (MQ=255)
                                                                                                              gCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCt                                  >  2:181552/1‑148 (MQ=255)
                                                                                                                aGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCGTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGCGCCCCACGACAGGCCCCCGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCTTCt                               >  5:178098/1‑149 (MQ=255)
                                                                                                                  aaaaaaaaGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTcg                                                                                                                        <  5:2063/59‑1 (MQ=255)
                                                                                                                  aaaaaaaaGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTcg                                                                                                                        >  6:2063/1‑59 (MQ=255)
                                                                                                                   cctaaaaGCGCCCGGCGGCCACCGCCCGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCTTCTg                              >  6:113735/4‑147 (MQ=39)
                                                                                                                   aaaaaaaGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCg                                            >  2:204700/1‑133 (MQ=255)
                                                                                                                   aaaaaaaGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCg                                            <  1:204700/133‑1 (MQ=255)
                                                                                                                     taaaaGCGCCCGGCGGCCACCGCCCGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCa                                                                                                 <  3:182987/78‑1 (MQ=25)
                                                                                                                     taaaaGCGCCCGGCGGCCACCGCCCGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCa                                                                                                 >  4:182987/2‑79 (MQ=25)
                                                                                                                        aaGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCTTCTgcagcagc                       <  2:114272/149‑1 (MQ=255)
                                                                                                                        aaGCGCCCGGCGGCCACCGCCCGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGc                                   <  1:35299/137‑1 (MQ=255)
                                                                                                                        aaGCGCCCGGCGGCCACCGCCCGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGc                                   >  2:35299/1‑137 (MQ=255)
                                                                                                                            gcCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCTTCTGCAGCAGCAGCg                   <  1:76431/149‑1 (MQ=255)
                                                                                                                             ccGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTAGCCATCGAGCATGTGCTTCTGCAGCAGCAGCg                   <  1:42940/149‑1 (MQ=255)
                                                                                                                                  cGGTGGCCGCCGGGCGCTTTTAGGGGCTTACTGGGAGCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCTGCTGCAGCAGCAGCGGAacac             <  6:178098/149‑1 (MQ=255)
                                                                                                                                    gTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAACACTg           <  8:39845/149‑1 (MQ=255)
                                                                                                                                       gccgccGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAACACTggag        <  5:143761/149‑1 (MQ=255)
                                                                                                                                             gggCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAACACTGGAGGACAcc  >  1:223581/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                     
AGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTTTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTATTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCT‑GCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAACACTGGAGGACACC  >  NC_002947/4970470‑4970759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑