Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,010,534 C→A 5.6% A15E (GCG→GAG)  PP_4415 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,010,5340CA 5.6% 228.6 / 3.1 72A15E (GCG→GAG) PP_4415hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (39/29);  new base A (2/2);  total (41/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

TATACTCGCAACATACTGATATCTTGTCGGATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAGCTGATCGACTACCTAGAGC  >  NC_002947/5010386‑5010680
                                                                                                                                                     |                                                                                                                                                  
tataCTCGCAACATACTGATATCTTGTCGGATTCCTCCCCGATAGGCTTGACCATTCTCGATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGgc                                                                                                                                                    >  8:146782/1‑149 (MQ=255)
     tCGCAACATACTGATATCTTGTCGGATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTc                                                                                                                                                >  1:207166/1‑148 (MQ=255)
     tCGCAACATACTGATATCATGTCGGATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTc                                                                                                                                                >  8:62173/1‑148 (MQ=255)
       gCAACATACTGATATCTTGTCGGATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAAc                                                                                                                                             >  7:60858/1‑149 (MQ=255)
           cATACTGATATCTTGTCGGATTCCTCCCCGATCGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCg                                                                                                                                         <  2:302608/149‑1 (MQ=255)
                  atatCTTGTCGGATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACaaa                                                                                                                                  >  1:239368/1‑149 (MQ=255)
                    atCTTGTCGGATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGAACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAAc                                                                                                                                 >  2:30410/1‑148 (MQ=255)
                       ttGTCGGATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTc                                                                                                                                       <  3:106571/139‑1 (MQ=255)
                       ttGTCGGATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTc                                                                                                                                       >  4:106571/1‑139 (MQ=255)
                       ttGTCGGATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACg                                                                                                                             >  8:133050/1‑149 (MQ=255)
                        tGTCGGATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACgt                                                                                                                            >  6:363065/1‑149 (MQ=255)
                            ggATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACgtggt                                                                                                                         <  8:60858/148‑1 (MQ=255)
                             gATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTTGAGCACCACGGCGCca                                                                                                                                      >  3:69290/1‑134 (MQ=255)
                             gATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTTGAGCACCACGGCGCca                                                                                                                                      <  4:69290/134‑1 (MQ=255)
                                  ctccCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAAt                                                                                                                  <  7:62173/149‑1 (MQ=255)
                                     cccGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGc                                                                                                               <  5:363065/149‑1 (MQ=255)
                                             gCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGgc                                                                                                                                                    >  2:285540/1‑104 (MQ=255)
                                             gCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGgc                                                                                                                                                    <  1:285540/104‑1 (MQ=255)
                                             gCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATg                                                                                                       >  1:243850/1‑149 (MQ=255)
                                               ttGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGAGGGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCGCAAACTACTTGGTGCTGAATTGCCTGCAATg                                                                                                       >  1:270061/1‑148 (MQ=255)
                                                    cATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCg                                                                                                >  2:105490/1‑149 (MQ=255)
                                                    cATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCg                                                                                                >  1:335135/1‑149 (MQ=255)
                                                    cATTCTACATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTACCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCg                                                                                                >  2:85382/1‑149 (MQ=255)
                                                     aTTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTACAATGGAAGTCGc                                                                                               >  1:123229/1‑149 (MQ=255)
                                                      ttCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTTCCTGCAAt                                                                                                        >  4:144332/1‑139 (MQ=255)
                                                      ttCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAAt                                                                                                        <  3:144332/139‑1 (MQ=255)
                                                           cATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCcac                                                                                            <  2:239368/146‑1 (MQ=255)
                                                            aTCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGc                                                                                               <  2:126111/142‑1 (MQ=255)
                                                            aTCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGc                                                                                               >  1:126111/1‑142 (MQ=255)
                                                            aTCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCt                                                                                        >  7:284878/1‑149 (MQ=255)
                                                                gtcgtgTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTc                                                                                       >  2:180370/1‑146 (MQ=255)
                                                                    tcTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGc                                                                                <  8:218124/149‑1 (MQ=255)
                                                                        gATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTg                                                                              <  7:146782/147‑1 (MQ=255)
                                                                        gATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGc                                                                            >  4:252073/1‑149 (MQ=255)
                                                                            ttCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTcg                                                                        >  5:55751/1‑149 (MQ=255)
                                                                                ggTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTcggcgg                                                                    <  2:243850/149‑1 (MQ=255)
                                                                                  tACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCggcggc                                                                   >  3:352515/1‑148 (MQ=255)
                                                                                   aCCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGcc                                                                  >  5:348943/1‑148 (MQ=255)
                                                                                       aTTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCa                                                             <  2:207166/149‑1 (MQ=255)
                                                                                        tttGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCag                                                            <  1:180370/149‑1 (MQ=255)
                                                                                        tttGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCag                                                            <  1:105490/149‑1 (MQ=255)
                                                                                           ggggA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACGTCGAGATGCTGGCTTCGGCGGCCAAGCagag                                                          <  7:303781/148‑1 (MQ=255)
                                                                                              gA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTc                                                      >  3:148386/1‑149 (MQ=255)
                                                                                                    aCCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGACGCCGTTGAGCGCCACGGCgcc                                                                                                                                       <  1:254049/63‑3 (MQ=21)
                                                                                                    aCCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGACGCCGTTGAGCGCCACGGCgcc                                                                                                                                       >  2:254049/1‑61 (MQ=21)
                                                                                                     ccTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGaa                                                                                                    >  3:329198/1‑97 (MQ=255)
                                                                                                     ccTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGaa                                                                                                    <  4:329198/97‑1 (MQ=255)
                                                                                                     ccTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGt                                                 >  2:248566/1‑148 (MQ=255)
                                                                                                      cTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGCCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGa                                               >  4:18061/1‑149 (MQ=255)
                                                                                                           tGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTAc                                          <  4:352515/149‑1 (MQ=255)
                                                                                                             cccACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACg                                         <  1:248566/148‑1 (MQ=255)
                                                                                                                aCTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGaa                                     >  5:317133/1‑149 (MQ=255)
                                                                                                                aCTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGaa                                     >  7:211640/1‑149 (MQ=255)
                                                                                                                  ttCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAAc                                    >  3:268172/1‑148 (MQ=255)
                                                                                                                    cAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATgg                                                                                                      <  3:142884/80‑1 (MQ=255)
                                                                                                                    cAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATgg                                                                                                      >  4:142884/1‑80 (MQ=255)
                                                                                                                    cAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACg                                   <  4:268172/147‑1 (MQ=255)
                                                                                                                     aaTTTTGTACCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTTCGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCTCACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGc                                <  1:85382/149‑1 (MQ=255)
                                                                                                                           gTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTcg                                                     <  2:162520/122‑1 (MQ=255)
                                                                                                                           gTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTcg                                                     >  1:162520/1‑122 (MQ=255)
                                                                                                                             tCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCa                         <  3:18061/148‑1 (MQ=255)
                                                                                                                              ccAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAg                        >  3:337606/1‑148 (MQ=255)
                                                                                                                               cAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGc                                >  6:6071/1‑139 (MQ=255)
                                                                                                                               cAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGc                                <  5:6071/139‑1 (MQ=255)
                                                                                                                                aGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAg                     <  2:335135/149‑1 (MQ=255)
                                                                                                                                aGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAg                     >  4:14195/1‑149 (MQ=255)
                                                                                                                                aGCTTGAGCATGGCGCCGTGGCGCTCAACGCCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCATCCCGGTGATTTACGAGAACGAGCATGACCAGGAg                     >  6:41247/1‑149 (MQ=255)
                                                                                                                                  cTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAGCt                   <  7:133050/149‑1 (MQ=255)
                                                                                                                                   ttGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCgag                                                                                    <  1:203029/83‑1 (MQ=255)
                                                                                                                                   ttGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCgag                                                                                    >  2:203029/1‑83 (MQ=255)
                                                                                                                                   ttGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAGCt                   >  3:200241/1‑148 (MQ=255)
                                                                                                                                      aGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAGCTGAt                <  4:148386/148‑1 (MQ=255)
                                                                                                                                        cATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAGCTGATCGa             <  1:266998/149‑1 (MQ=255)
                                                                                                                                          tGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAGCTGATCGAc            >  6:137433/1‑148 (MQ=255)
                                                                                                                                           ggCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAGCTGATCGACTa          >  7:339846/1‑149 (MQ=255)
                                                                                                                                                    gcgcTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAGCTGATCGACTACCTAGAGc  <  2:123229/148‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TATACTCGCAACATACTGATATCTTGTCGGATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAGCTGATCGACTACCTAGAGC  >  NC_002947/5010386‑5010680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑