Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,010,539 A→C 5.4% N17H (AAC→CAC)  PP_4415 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,010,5390AC 5.4% 226.7 / 2.5 73N17H (AAC→CAC) PP_4415hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (38/31);  new base C (2/2);  total (40/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

GCAACATACTGATATCTTGTCGGATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAGCTGATCGACTACCTAGAGCGGGTACA  >  NC_002947/5010393‑5010687
                                                                                                                                                   |                                                                                                                                                    
gCAACATACTGATATCTTGTCGGATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAAc                                                                                                                                                    >  7:60858/1‑149 (MQ=255)
    cATACTGATATCTTGTCGGATTCCTCCCCGATCGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCg                                                                                                                                                <  2:302608/149‑1 (MQ=255)
           atatCTTGTCGGATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACaaa                                                                                                                                         >  1:239368/1‑149 (MQ=255)
             atCTTGTCGGATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGAACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAAc                                                                                                                                        >  2:30410/1‑148 (MQ=255)
                ttGTCGGATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTc                                                                                                                                              <  3:106571/139‑1 (MQ=255)
                ttGTCGGATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTc                                                                                                                                              >  4:106571/1‑139 (MQ=255)
                ttGTCGGATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACg                                                                                                                                    >  8:133050/1‑149 (MQ=255)
                 tGTCGGATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACgt                                                                                                                                   >  6:363065/1‑149 (MQ=255)
                     ggATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACgtggt                                                                                                                                <  8:60858/148‑1 (MQ=255)
                      gATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTTGAGCACCACGGCGCca                                                                                                                                             <  4:69290/134‑1 (MQ=255)
                      gATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTTGAGCACCACGGCGCca                                                                                                                                             >  3:69290/1‑134 (MQ=255)
                           ctccCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAAt                                                                                                                         <  7:62173/149‑1 (MQ=255)
                              cccGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGc                                                                                                                      <  5:363065/149‑1 (MQ=255)
                                      gCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATg                                                                                                              >  1:243850/1‑149 (MQ=255)
                                        ttGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGAGGGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCGCAAACTACTTGGTGCTGAATTGCCTGCAATg                                                                                                              >  1:270061/1‑148 (MQ=255)
                                             cATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCg                                                                                                       >  1:335135/1‑149 (MQ=255)
                                             cATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCg                                                                                                       >  2:105490/1‑149 (MQ=255)
                                             cATTCTACATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTACCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCg                                                                                                       >  2:85382/1‑149 (MQ=255)
                                              aTTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTACAATGGAAGTCGc                                                                                                      >  1:123229/1‑149 (MQ=255)
                                               ttCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTTCCTGCAAt                                                                                                               >  4:144332/1‑139 (MQ=255)
                                               ttCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAAt                                                                                                               <  3:144332/139‑1 (MQ=255)
                                                    cATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCcac                                                                                                   <  2:239368/146‑1 (MQ=255)
                                                     aTCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGc                                                                                                      >  1:126111/1‑142 (MQ=255)
                                                     aTCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGc                                                                                                      <  2:126111/142‑1 (MQ=255)
                                                     aTCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCt                                                                                               >  7:284878/1‑149 (MQ=255)
                                                         gtcgtgTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTc                                                                                              >  2:180370/1‑146 (MQ=255)
                                                             tcTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGc                                                                                       <  8:218124/149‑1 (MQ=255)
                                                                 gATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTg                                                                                     <  7:146782/147‑1 (MQ=255)
                                                                 gATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGc                                                                                   >  4:252073/1‑149 (MQ=255)
                                                                     ttCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTcg                                                                               >  5:55751/1‑149 (MQ=255)
                                                                         ggTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTcggcgg                                                                           <  2:243850/149‑1 (MQ=255)
                                                                           tACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCggcggc                                                                          >  3:352515/1‑148 (MQ=255)
                                                                            aCCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGcc                                                                         >  5:348943/1‑148 (MQ=255)
                                                                                aTTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCa                                                                    <  2:207166/149‑1 (MQ=255)
                                                                                 tttGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCag                                                                   <  1:180370/149‑1 (MQ=255)
                                                                                 tttGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCag                                                                   <  1:105490/149‑1 (MQ=255)
                                                                                    ggggA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACGTCGAGATGCTGGCTTCGGCGGCCAAGCagag                                                                 <  7:303781/148‑1 (MQ=255)
                                                                                       gA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTc                                                             >  3:148386/1‑149 (MQ=255)
                                                                                             aCCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGACGCCGTTGAGCGCCACGGCgcc                                                                                                                                              >  2:254049/1‑61 (MQ=21)
                                                                                             aCCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGACGCCGTTGAGCGCCACGGCgcc                                                                                                                                              <  1:254049/63‑3 (MQ=21)
                                                                                              ccTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGaa                                                                                                           >  3:329198/1‑97 (MQ=255)
                                                                                              ccTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGaa                                                                                                           <  4:329198/97‑1 (MQ=255)
                                                                                              ccTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGt                                                        >  2:248566/1‑148 (MQ=255)
                                                                                               cTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGCCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGa                                                      >  4:18061/1‑149 (MQ=255)
                                                                                                    tGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTAc                                                 <  4:352515/149‑1 (MQ=255)
                                                                                                      cccACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACg                                                <  1:248566/148‑1 (MQ=255)
                                                                                                         aCTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGaa                                            >  7:211640/1‑149 (MQ=255)
                                                                                                         aCTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGaa                                            >  5:317133/1‑149 (MQ=255)
                                                                                                           ttCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAAc                                           >  3:268172/1‑148 (MQ=255)
                                                                                                             cAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATgg                                                                                                             >  4:142884/1‑80 (MQ=255)
                                                                                                             cAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATgg                                                                                                             <  3:142884/80‑1 (MQ=255)
                                                                                                             cAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACg                                          <  4:268172/147‑1 (MQ=255)
                                                                                                              aaTTTTGTACCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTTCGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCTCACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGc                                       <  1:85382/149‑1 (MQ=255)
                                                                                                                    gTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTcg                                                            <  2:162520/122‑1 (MQ=255)
                                                                                                                    gTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTcg                                                            >  1:162520/1‑122 (MQ=255)
                                                                                                                      tCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCa                                <  3:18061/148‑1 (MQ=255)
                                                                                                                       ccAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAg                               >  3:337606/1‑148 (MQ=255)
                                                                                                                        cAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGc                                       <  5:6071/139‑1 (MQ=255)
                                                                                                                        cAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGc                                       >  6:6071/1‑139 (MQ=255)
                                                                                                                         aGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAg                            <  2:335135/149‑1 (MQ=255)
                                                                                                                         aGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAg                            >  4:14195/1‑149 (MQ=255)
                                                                                                                         aGCTTGAGCATGGCGCCGTGGCGCTCAACGCCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCATCCCGGTGATTTACGAGAACGAGCATGACCAGGAg                            >  6:41247/1‑149 (MQ=255)
                                                                                                                           cTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAGCt                          <  7:133050/149‑1 (MQ=255)
                                                                                                                            ttGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCgag                                                                                           <  1:203029/83‑1 (MQ=255)
                                                                                                                            ttGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCgag                                                                                           >  2:203029/1‑83 (MQ=255)
                                                                                                                            ttGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAGCt                          >  3:200241/1‑148 (MQ=255)
                                                                                                                               aGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAGCTGAt                       <  4:148386/148‑1 (MQ=255)
                                                                                                                                 cATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAGCTGATCGa                    <  1:266998/149‑1 (MQ=255)
                                                                                                                                   tGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAGCTGATCGAc                   >  6:137433/1‑148 (MQ=255)
                                                                                                                                    ggCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAGCTGATCGACTa                 >  7:339846/1‑149 (MQ=255)
                                                                                                                                             gcgcTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAGCTGATCGACTACCTAGAGc         <  2:123229/148‑1 (MQ=255)
                                                                                                                                               gcTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCtt                                     <  7:179522/118‑1 (MQ=255)
                                                                                                                                               gcTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCtt                                     >  8:179522/1‑118 (MQ=255)
                                                                                                                                                   aaCGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAGCTGATCGACTACCTAGAGCTGGTACa  >  8:22029/1‑149 (MQ=255)
                                                                                                                                                   aaCGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAGCTGATCGACTACCTAGAGCGGGTAc   <  6:55751/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCAACATACTGATATCTTGTCGGATTCCTCCCCGATAGGCTTGACCATTCTCCATCCGTCGTCTTGATTTTCAGGTACCAATTTGGGGA‑GGTACCTGCATGCCCACTTCAATTTTGTTCCAGCTTGAGCATGGCGCCGTGGCGCTCAACGGCGTCACAAACTACGTGGTGCTGAATTGCCTGCAATGGAAGTCGCCACACCTCGAGATGCTGGCTTCGGCGGCCAAGCAGAGCTTCGCGGTGATTTACGAGAACGAGCTTGACCAGGAGCTGATCGACTACCTAGAGCGGGTACA  >  NC_002947/5010393‑5010687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑