Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,019,896 G→T 100% A72A (GCC→GCA)  PP_4423 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,019,8960GT100.0% 130.4 / NA 41A72A (GCC→GCA) PP_4423hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/22);  total (19/22)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACTGTCCCGTCAGCCTTACCTGGGAAGGTACGATTCAGATTCCCGCGATCAATCGGAGAGGTTCTGGAGGCGTTTTTAAAAGCAGGATAGTTCATGAAGGGCAAAATAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTGGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGCCGATCCCGTTTTTCATGACGGTGATAGGAATCATGATCGCACCCCACGCAGAGTCATCACGTGAGTAAGGC  >  NC_002947/5019750‑5020040
                                                                                                                                                  |                                                                                                                                                
aCTGTCCCGTCAGCCTTACCTGGGAAGGTACGATTCAGATTCCCGCGATCAATCGGAGAGGTTCTGGAGGCGTTTTTAAAAGCAGGATAGTTCATGAAGGGCAAAATAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTTGc                                                                                                                                                >  1:246999/1‑149 (MQ=255)
  tGTCCCGTCAGCCTTACCTGGGAAGGTACGATTCAGATTCCCGCGATCAATCGGAGAGGTTCTGGAGGCGTTTTTAAAAGCAGGATAGTTCATGAAGGGCAAAATAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTTGc                                                                                                                                                >  7:167638/1‑147 (MQ=255)
             ccTTACCTGGGAAGGTACGATTCAGATTCCCGCGATCAATCGGAGAGGTTCTGGAGGCGTTTTTAAAAGCAGGATAGTTCATGAAGGGCAAAATAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTTGCCAGCTTGGTGAGt                                                                                                                                   <  8:167638/149‑1 (MQ=255)
              cTTACCTGGGAAGGTACGATTCAGATTCCCGCGATCAATCGGAGAGGTTCTGGAGGCGTTTTTAAAAGCAGGATAGTTCATGAAGGGCAAAATAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTg                                                                                                                                  <  1:343448/149‑1 (MQ=255)
                  ccTGGGAAGGTACGATTCAGATTCCCGCGATCAATCGGAGAGGTTCTGGAGGCGTTTTTAAAAGCAGGATAGTTCATGAAGGGCAAAATAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAAt                                                                                                                              <  8:278827/149‑1 (MQ=255)
                              cGATTCAGATTCCCGCGATCAATCGGAGAGGTTCTGGAGGCGTTTTTAAAAGCAGGATAGTTCATGAAGGGCAAAATAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATa                                                                                                                  <  1:86886/149‑1 (MQ=255)
                                 ttCAGATTCCCGCGATCAATCGGAGAGGTTCTGGAGGCGTTTTTAAAAGCAGGATAGTTCATGAAGGGCAAAATAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTc                                                                                                               <  6:237860/149‑1 (MQ=255)
                                     gATTCCCGCGATCAATCGGAGAGGTTCTGGAGGCGTTTTTAAAAGCAGGATAGTTCATGAAGGGCAAAATAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTTGCCAGCTTTGTGAGTGCAATCGGGCCTTCATACTCGtct                                                                                                           >  1:56866/1‑149 (MQ=255)
                                             cgATCAATCGGAGAGGTTCTGGAGGCGTTTTTAAAAGCAGGATAGTTCATGAAGGGCAAAATAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTTGCCAGCTTGGt                                                                                                                                       >  1:88186/1‑113 (MQ=255)
                                             cgATCAATCGGAGAGGTTCTGGAGGCGTTTTTAAAAGCAGGATAGTTCATGAAGGGCAAAATAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTTGCCAGCTTGGt                                                                                                                                       <  2:88186/113‑1 (MQ=255)
                                                tCAATCGGAGAGGTTCTGGAGGCGTTTTTAAAAGCAGGATAGTTCATGAAGGGCAAAATAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTgcc                                                                                                <  2:246999/149‑1 (MQ=255)
                                                  aaTCGGAGAGGTTCTGGAGGCGTTTTTAAAAGCAGGATAGTTCATGAAGGGCAAAATAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTgccgc                                                                                              >  2:102314/1‑149 (MQ=255)
                                                      ggAGAGGTTCTGGAGGCGTTTTTAAAAGCAGGATAGTTCATGAAGGGCAAAATAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATAATTGCCGCCAg                                                                                           <  2:299328/148‑1 (MQ=255)
                                                       gagaGGTTCTGGAGGCGTTTTTATAAGCAGGATAGTTCATGAAGGGCAAATTAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTg                                                                                         <  5:172462/149‑1 (MQ=255)
                                                                             aataGCAGGATAGTTCATGAAGGGCAAAATTATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTTGCCAGCTTTGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGCCGATc                                                                    <  1:160222/145‑1 (MQ=255)
                                                                                   ggatctgTTCATGAAGGGCAAAATAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGACCACAGTCGGGCCGATCCCGttt                                                              <  7:227467/142‑1 (MQ=255)
                                                                                    ggATAGTTCATGAAGGGCAAAATAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTTGCCAGCTTTGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGCCGATCCCGttttt                                                            <  2:56866/149‑1 (MQ=255)
                                                                                     gATAGTTCATGAAGGGCAAAATAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGCCGATCCCGttttt                                                            >  1:170035/1‑148 (MQ=255)
                                                                                          ttCATGAAGGGCAAAATAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTTGCCAGCGTGGTGAGTGCAATCGGGCGTTCATACTGGTCTGCATGATTGCCGCCAGTGAGGAGCACAGTCGGGCCGATCCCGttttaggtcg                                                       >  8:227467/1‑142 (MQ=255)
                                                                                            cATGAAGGGCAAAATAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGc                                                                         >  6:226646/1‑128 (MQ=255)
                                                                                            cATGAAGGGCAAAATAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGc                                                                         <  5:226646/128‑1 (MQ=255)
                                                                                                  gggCAAAATAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGCCGATCCCGTTTTTCATGACGGTGATAg                                              >  2:92911/1‑149 (MQ=255)
                                                                                                                   gCCCTCTGACTGTTTCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGCCGATCCCGTTTTTCATGACGGTGATAGGAATCATGATCGCAccc                             >  5:358221/1‑149 (MQ=255)
                                                                                                                        ctGACTGTTTCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGCCGATCCCGTTTTTCATGACGGTGATAg                                              <  3:241269/127‑1 (MQ=255)
                                                                                                                        ctGACTGTTTCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGCCGATCCCGTTTTTCATGACGGTGATAg                                              >  4:241269/1‑127 (MQ=255)
                                                                                                                          gACTGTTTCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTgagg                                                                                      >  4:263496/1‑85 (MQ=255)
                                                                                                                          gACTGTTTCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTgagg                                                                                      <  3:263496/85‑1 (MQ=255)
                                                                                                                           aCTGTTTCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGCCGATCCCGTTTTTCATGACGGTGATAGGAATCATGATCGCACCCCACGCa                       >  7:109830/1‑147 (MQ=255)
                                                                                                                               tttCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGCCGATCCCGTTTTTCATGACGGTGATAGGAATCATGATCGCACCCCACGc                        >  5:99047/1‑142 (MQ=255)
                                                                                                                               tttCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGCCGATCCCGTTTTTCATGACGGTGATAGGAATCATGATCGCACCCCACGc                        <  6:99047/142‑1 (MQ=255)
                                                                                                                                ttCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGCCGATCCCGTTTTTCATGACGGTGATAGGAATCATGATCGCACCCCACGCag                      >  2:178536/1‑143 (MQ=255)
                                                                                                                                ttCGATGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGCCGATCCCGTTTTTCATGACGGTGATAGGAATCATGATCGCACCCCACGCag                      <  1:178536/143‑1 (MQ=255)
                                                                                                                                     tGTCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGCCGTTCCCGTTTTTCATGACGGTGATAGGAATCATGATCGCACCCCACGCAGAGTCATCACGt           <  1:102314/149‑1 (MQ=255)
                                                                                                                                       tCCAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGCCGATCCCGTTTTTCATGACGGTGATAGGAATCATGATCGCACCCCACGCAGAGTCATCACGTGa         <  6:358221/149‑1 (MQ=255)
                                                                                                                                         cAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTgagga                                                                                     >  6:160410/1‑71 (MQ=255)
                                                                                                                                         cAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTgagga                                                                                     <  5:160410/71‑1 (MQ=255)
                                                                                                                                         cAGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGCCGCTCCCGTTTTTCATGACGGTGATAGGAATCATGATCGCACCCCACGCAGAGTCATCACGTGAg        >  8:76392/1‑148 (MQ=255)
                                                                                                                                          aGTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGCCGAACCCGTTTTTCATGACGGTGATAGGAATCATGATCGCACCCCACGCAGAGTCAACACGTGAga       >  7:283770/1‑147 (MQ=255)
                                                                                                                                           gTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGCCGATCCCGTTTTTCATGa                                                       <  6:216602/99‑1 (MQ=255)
                                                                                                                                           gTTGATTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGCCGATCCCGTTTTTCATGa                                                       >  5:216602/1‑99 (MQ=255)
                                                                                                                                               aTTTGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGCCGATCCCGTTTTTCATGACGGTGATAGGAATCATGATCGCACCCCACGCAGAGTCATCACGTGAGTAAGGc  <  1:92911/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ACTGTCCCGTCAGCCTTACCTGGGAAGGTACGATTCAGATTCCCGCGATCAATCGGAGAGGTTCTGGAGGCGTTTTTAAAAGCAGGATAGTTCATGAAGGGCAAAATAATGATTCGCCCTCTGACTGTTTCGATGTCCAGTTGATTGGCCAGCTTGGTGAGTGCAATCGGGCCTTCATACTCGTCTCCATGATTGCCGCCAGTGAGGAGCACAGTCGGGCCGATCCCGTTTTTCATGACGGTGATAGGAATCATGATCGCACCCCACGCAGAGTCATCACGTGAGTAAGGC  >  NC_002947/5019750‑5020040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑