Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,026,750 A→T 7.4% T20T (ACA→ACT)  ocd → ornithine cyclodeaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,026,7500AT 7.4% 161.7 / 2.9 54T20T (ACA→ACT) ocdornithine cyclodeaminase
Reads supporting (aligned to +/- strand):  ref base A (22/28);  new base T (2/2);  total (24/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

GGGAATGTCGACATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCTTGAGAATTT  >  NC_002947/5026605‑5026893
                                                                                                                                                 |                                                                                                                                               
gggAATGTCGACATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATacagac                                                                                                                                              <  6:1035/149‑1 (MQ=255)
      gTCGACATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATc                                                                                                                                        >  7:266340/1‑149 (MQ=255)
         gACATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGAt                                                                                                                                     <  5:65793/149‑1 (MQ=255)
                 tCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTgg                                                                                                                              >  4:61759/1‑148 (MQ=255)
                  cAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGGGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGa                                                                                                                             >  1:270984/1‑148 (MQ=255)
                    acacCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGaaaa                                                                                                                          <  8:266340/149‑1 (MQ=255)
                       ccAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGc                                                                                                                       <  4:32931/149‑1 (MQ=255)
                         aaCGCATCCTCCTCGAAGCTGTCCGCGACCGCCGCCCAGCACGTGCTTAAAGACGCGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGGGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCAt                                                                                                                     <  4:295148/149‑1 (MQ=255)
                          aCGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCAtt                                                                                                                    >  3:158830/1‑149 (MQ=255)
                            gCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTg                                                                                                                  >  2:3640/1‑149 (MQ=255)
                                 atcCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCg                                                                                                             >  3:208741/1‑149 (MQ=255)
                                    cTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCt                                                                                                           <  3:71167/148‑1 (MQ=255)
                                    cTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCAATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCt                                                                                                           <  2:139196/148‑1 (MQ=255)
                                     cacgtgCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTgg                                                                                                         >  1:83744/6‑149 (MQ=255)
                                       gAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGc                                                                                                        <  2:65832/148‑1 (MQ=255)
                                        aaGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCa                                                                                                      <  6:147262/149‑1 (MQ=255)
                                           cTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCagag                                                                                                   >  3:333439/1‑149 (MQ=255)
                                                cgcgACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCTGAGGCa                                                                                                >  8:241104/1‑147 (MQ=255)
                                                   gACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTg                                                                                           <  4:333439/149‑1 (MQ=255)
                                                    aCCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTgg                                                                                          <  3:147291/149‑1 (MQ=255)
                                                    aCCGCAGCCCAGCACGTGCTTAAAGTGGTGCCATGTTAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCATAGGCAAAGTgg                                                                                          <  3:179642/149‑1 (MQ=255)
                                                        ccgccCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCAt                                                                                      >  5:200309/1‑149 (MQ=255)
                                                         cgccCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATg                                                                                     >  4:350269/1‑149 (MQ=255)
                                                             cAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGt                                                                                 >  5:76200/1‑149 (MQ=255)
                                                                             agatGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGa                                                                 <  5:245997/146‑1 (MQ=255)
                                                                              ggTGCCATGTGATCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGa                                                                 <  1:277794/148‑1 (MQ=255)
                                                                              ggTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGAc                                                                <  2:270984/149‑1 (MQ=255)
                                                                                  ccATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACAtct                                                            >  5:159773/1‑149 (MQ=255)
                                                                                       tgAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGa                                                        <  2:83744/148‑1 (MQ=255)
                                                                                             aaGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCa                                                                                       <  6:307249/111‑1 (MQ=255)
                                                                                             aaGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCa                                                                                       >  5:307249/1‑111 (MQ=255)
                                                                                             aaGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGt                                                                     >  2:187251/1‑129 (MQ=255)
                                                                                             aaGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGt                                                                     <  1:187251/129‑1 (MQ=255)
                                                                                               gTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATgg                                               <  6:76200/149‑1 (MQ=255)
                                                                                                        cTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACAt                                                              >  4:202610/1‑125 (MQ=255)
                                                                                                        cTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACAt                                                              <  3:202610/125‑1 (MQ=255)
                                                                                                           aaCCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGAc                                     <  3:61759/147‑1 (MQ=255)
                                                                                                           aaCCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGAc                                     <  6:200309/147‑1 (MQ=255)
                                                                                                              cAACGCGAAATCCGTGAGTGCGTAACGCCCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCa                                 >  2:126979/1‑148 (MQ=255)
                                                                                                                     aaaTCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCt                         >  1:96446/1‑149 (MQ=255)
                                                                                                                              aGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGcc                 <  4:101033/148‑1 (MQ=255)
                                                                                                                              aGTGCGTAACGCCCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCa                <  1:126979/149‑1 (MQ=255)
                                                                                                                               gTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGcc                                                                                   <  5:135072/81‑1 (MQ=255)
                                                                                                                               gTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGcc                                                                                   >  6:135072/1‑81 (MQ=255)
                                                                                                                                tGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGa              >  6:97182/1‑149 (MQ=255)
                                                                                                                                  cGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGAc             <  2:96446/148‑1 (MQ=255)
                                                                                                                                      tacatCGATACTGTCTGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGTCAAAGTGGTCATGCAGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACTTCAACACCGCATACCTGCCAGACAtt          >  6:88306/5‑147 (MQ=255)
                                                                                                                                      tacatCGATACTGTCTGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCGGAGCTCAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCGAGACCTTga        <  5:88306/145‑1 (MQ=255)
                                                                                                                                      aCGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCTTga        <  4:315335/149‑1 (MQ=255)
                                                                                                                                      aCGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCTTga        <  4:158830/149‑1 (MQ=255)
                                                                                                                                      aCGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCTTga        <  5:363516/149‑1 (MQ=255)
                                                                                                                                        gCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCTTgag       >  2:239181/1‑148 (MQ=255)
                                                                                                                                        catCGATACTGTCTGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCt           <  3:165112/142‑1 (MQ=255)
                                                                                                                                        catCGATACTGTCTGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCt           >  4:165112/3‑144 (MQ=255)
                                                                                                                                            gATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCTTGAGAAttt  >  8:297423/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GGGAATGTCGACATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCTTGAGAATTT  >  NC_002947/5026605‑5026893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑