Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,026,752	A→T	7.4%	D21V (GAC→GTC)	ocd →	ornithine cyclodeaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,026,752	0	A	T	7.4%	158.0 / 2.9	54	D21V (GAC→GTC)	ocd	ornithine cyclodeaminase
Reads supporting (aligned to +/- strand):  ref base A (22/28);  new base T (2/2);  total (24/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

GGGAATGTCGACATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCTTGAGAATTTC  >  NC_002947/5026605‑5026894                                                                                                                                                    |                                                                                                                                               gggAATGTCGACATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATacagac                                                                                                                                               <  6:1035/149‑1 (MQ=255)       gTCGACATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATc                                                                                                                                         >  7:266340/1‑149 (MQ=255)          gACATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGAt                                                                                                                                      <  5:65793/149‑1 (MQ=255)                  tCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTgg                                                                                                                               >  4:61759/1‑148 (MQ=255)                   cAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGGGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGa                                                                                                                              >  1:270984/1‑148 (MQ=255)                     acacCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGaaaa                                                                                                                           <  8:266340/149‑1 (MQ=255)                        ccAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGc                                                                                                                        <  4:32931/149‑1 (MQ=255)                          aaCGCATCCTCCTCGAAGCTGTCCGCGACCGCCGCCCAGCACGTGCTTAAAGACGCGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGGGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCAt                                                                                                                      <  4:295148/149‑1 (MQ=255)                           aCGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCAtt                                                                                                                     >  3:158830/1‑149 (MQ=255)                             gCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTg                                                                                                                   >  2:3640/1‑149 (MQ=255)                                  atcCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCg                                                                                                              >  3:208741/1‑149 (MQ=255)                                     cTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCt                                                                                                            <  3:71167/148‑1 (MQ=255)                                     cTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCAATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCt                                                                                                            <  2:139196/148‑1 (MQ=255)                                      cacgtgCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTgg                                                                                                          >  1:83744/6‑149 (MQ=255)                                        gAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGc                                                                                                         <  2:65832/148‑1 (MQ=255)                                         aaGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCa                                                                                                       <  6:147262/149‑1 (MQ=255)                                            cTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCagag                                                                                                    >  3:333439/1‑149 (MQ=255)                                                 cgcgACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCTGAGGCa                                                                                                 >  8:241104/1‑147 (MQ=255)                                                    gACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTg                                                                                            <  4:333439/149‑1 (MQ=255)                                                     aCCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTgg                                                                                           <  3:147291/149‑1 (MQ=255)                                                     aCCGCAGCCCAGCACGTGCTTAAAGTGGTGCCATGTTAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCATAGGCAAAGTgg                                                                                           <  3:179642/149‑1 (MQ=255)                                                         ccgccCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCAt                                                                                       >  5:200309/1‑149 (MQ=255)                                                          cgccCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATg                                                                                      >  4:350269/1‑149 (MQ=255)                                                              cAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGt                                                                                  >  5:76200/1‑149 (MQ=255)                                                                              agatGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGa                                                                  <  5:245997/146‑1 (MQ=255)                                                                               ggTGCCATGTGATCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGa                                                                  <  1:277794/148‑1 (MQ=255)                                                                               ggTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGAc                                                                 <  2:270984/149‑1 (MQ=255)                                                                                   ccATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACAtct                                                             >  5:159773/1‑149 (MQ=255)                                                                                        tgAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGa                                                         <  2:83744/148‑1 (MQ=255)                                                                                              aaGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCa                                                                                        >  5:307249/1‑111 (MQ=255)                                                                                              aaGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCa                                                                                        <  6:307249/111‑1 (MQ=255)                                                                                              aaGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGt                                                                      >  2:187251/1‑129 (MQ=255)                                                                                              aaGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGt                                                                      <  1:187251/129‑1 (MQ=255)                                                                                                gTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATgg                                                <  6:76200/149‑1 (MQ=255)                                                                                                         cTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACAt                                                               >  4:202610/1‑125 (MQ=255)                                                                                                         cTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACAt                                                               <  3:202610/125‑1 (MQ=255)                                                                                                            aaCCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGAc                                      <  6:200309/147‑1 (MQ=255)                                                                                                            aaCCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGAc                                      <  3:61759/147‑1 (MQ=255)                                                                                                               cAACGCGAAATCCGTGAGTGCGTAACGCCCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCa                                  >  2:126979/1‑148 (MQ=255)                                                                                                                      aaaTCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCt                          >  1:96446/1‑149 (MQ=255)                                                                                                                               aGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGcc                  <  4:101033/148‑1 (MQ=255)                                                                                                                               aGTGCGTAACGCCCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCa                 <  1:126979/149‑1 (MQ=255)                                                                                                                                gTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGcc                                                                                    <  5:135072/81‑1 (MQ=255)                                                                                                                                gTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGcc                                                                                    >  6:135072/1‑81 (MQ=255)                                                                                                                                 tGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGa               >  6:97182/1‑149 (MQ=255)                                                                                                                                   cGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGAc              <  2:96446/148‑1 (MQ=255)                                                                                                                                       tacatCGATACTGTCTGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGTCAAAGTGGTCATGCAGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACTTCAACACCGCATACCTGCCAGACAtt           >  6:88306/5‑147 (MQ=255)                                                                                                                                       tacatCGATACTGTCTGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCGGAGCTCAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCGAGACCTTga         <  5:88306/145‑1 (MQ=255)                                                                                                                                       aCGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCTTga         <  5:363516/149‑1 (MQ=255)                                                                                                                                       aCGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCTTga         <  4:315335/149‑1 (MQ=255)                                                                                                                                       aCGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCTTga         <  4:158830/149‑1 (MQ=255)                                                                                                                                         gCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCTTgag        >  2:239181/1‑148 (MQ=255)                                                                                                                                         catCGATACTGTCTGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCt            >  4:165112/3‑144 (MQ=255)                                                                                                                                         catCGATACTGTCTGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCt            <  3:165112/142‑1 (MQ=255)                                                                                                                                             gATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCTTGAGAAttt   >  8:297423/1‑149 (MQ=255)                                                                                                                                                    acagTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCTTGAGAATTTc  >  2:50214/1‑143 (MQ=255)                                                                                                                                                    acagTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCTTGAGAATTTc  <  1:50214/143‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                               GGGAATGTCGACATGGCTCAACACCAACGCATCATCCTGGAAGCTGTGCGCGACCGCCGCCCAGCACGTGCTTAAAGAGGTGCCATGTGAGCGAAGTCCTTCTGCTTAACCAACGCGAAATCCGTGAGTGCGTAACGCTCGATACAGACAGTATCGATGTAGTGGAAAACGCATTTGCATCGCTGGCCAGAGGCAAAGTGGTCATGCCGTCAATCCTCCGGTTAGACATCTCCGAGCACAATGGCGAGGTGGACGTCAAAACCGCTTACCTGCCAGACCTTGAGAATTTC  >  NC_002947/5026605‑5026894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑