Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,059,505 A→T 9.3% N443K (AAT→AAA PP_4458 ← opine ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,059,5050AT 9.3% 203.4 / 8.5 73N443K (AAT→AAAPP_4458opine ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (35/31);  new base T (3/4);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCTGTTTCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGTAAG  >  NC_002947/5059359‑5059641
                                                                                                                                                  |                                                                                                                                         
aCCTGTTTCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAAtt                                                                                                                                         >  4:127678/1‑149 (MQ=255)
 ccttgttCAGTCCAAACAAAGGGATGGCGCATACGTCGGGGTGAATCTGAACCTGCGCCTTTTTCGACTACTCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTCCTGAGCAATGTCCGAAATTc                                                                                                                                        <  1:118009/144‑1 (MQ=255)
   tGTTTCAGACCAAACAAAGGGAGGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGAAGGCATCATCAGCTAGTGAGCAATGTCCGAAATTCGa                                                                                                                                     >  7:227561/1‑149 (MQ=255)
     tttCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCTGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGAc                                                                                                                                    <  8:176636/148‑1 (MQ=255)
     cctCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGa                                                                                                                                     <  8:227561/145‑1 (MQ=255)
          gACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTg                                                                                                                                >  5:166375/1‑147 (MQ=255)
           aCCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCGAATTTCGGACATTGctc                                                                                                                             <  3:94185/149‑3 (MQ=255)
             caaacaaaGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCg                                                                                                                           >  8:282530/1‑149 (MQ=255)
              aaacaaaGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCgg                                                                                                                          <  2:56314/149‑1 (MQ=255)
               aacaaaGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCggg                                                                                                                         >  7:303033/1‑149 (MQ=255)
                 caaaGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTg                                                                                                                       <  6:135459/149‑1 (MQ=255)
                  aaaGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTgc                                                                                                                      >  7:29259/1‑149 (MQ=255)
                  aaaGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTgc                                                                                                                      >  1:348426/1‑149 (MQ=255)
                           gcgcATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATgg                                                                                                              <  3:344529/148‑1 (MQ=255)
                           gcgcATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATgg                                                                                                              <  5:293432/148‑1 (MQ=255)
                                       tcgtGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATc                                                                                                 <  8:198440/149‑1 (MQ=255)
                                             aTCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAATTTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTaaaa                                                                                           <  5:160591/149‑1 (MQ=255)
                                                   aCCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGt                                                                                     <  6:166375/149‑1 (MQ=255)
                                                     cTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCa                                                                                   <  8:29259/149‑1 (MQ=255)
                                                       gcgcCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGc                                                                                 >  7:88562/1‑149 (MQ=255)
                                                        cgcCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGc                                                                                 <  6:354133/148‑1 (MQ=255)
                                                        cgcCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGAAGAAGCCGAATCGTAAAACTCTGTCAGc                                                                                 >  3:100618/1‑148 (MQ=255)
                                                                   acaAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAg                                                                                                                             <  3:223746/93‑1 (MQ=255)
                                                                   acaAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAg                                                                                                                             >  4:223746/1‑93 (MQ=255)
                                                                   acaAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAAt                                                                                                  >  5:64384/1‑120 (MQ=255)
                                                                   acaAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGgcg                                                                     >  5:290001/1‑149 (MQ=255)
                                                                   acaAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGgcg                                                                     >  1:181235/1‑149 (MQ=255)
                                                                   acaAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGgcg                                                                     >  4:41575/1‑149 (MQ=255)
                                                                   acaAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCCGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGgca                                                                     >  2:53586/1‑148 (MQ=255)
                                                                   acaAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTACGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGgcg                                                                     >  5:163207/1‑149 (MQ=255)
                                                                        tCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCgg                                                                <  7:282530/149‑1 (MQ=255)
                                                                            gTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGatat                                                            >  7:217585/1‑149 (MQ=255)
                                                                             ttGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATc                                                           <  8:88562/149‑1 (MQ=255)
                                                                                aGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGag                                                        <  1:160200/149‑1 (MQ=255)
                                                                                aGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGag                                                        <  3:127678/149‑1 (MQ=255)
                                                                                  tCTGCGGATCAGCCTCGCCCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGag                                                        >  6:273896/1‑147 (MQ=255)
                                                                                     gCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAgcagca                                                   >  4:120988/1‑149 (MQ=255)
                                                                                        gATCATCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCg                                                <  5:115593/149‑1 (MQ=255)
                                                                                             gCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGag                                                        <  1:76950/136‑1 (MQ=255)
                                                                                             gCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGag                                                        >  2:76950/1‑136 (MQ=255)
                                                                                              ccTCGACCCAGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAac                                          >  7:121180/1‑149 (MQ=255)
                                                                                               cTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAaca                                         >  3:67501/1‑149 (MQ=255)
                                                                                                tCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCg                                                                 <  6:13092/124‑1 (MQ=255)
                                                                                                tCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCg                                                                 >  5:13092/1‑124 (MQ=255)
                                                                                                     ccTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGc                                                                                 >  3:331338/1‑103 (MQ=255)
                                                                                                     ccTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGc                                                                                 <  4:331338/103‑1 (MQ=255)
                                                                                                         gCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGct                               >  7:279919/1‑149 (MQ=255)
                                                                                                           tttCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGctct                             >  5:274904/1‑149 (MQ=255)
                                                                                                             tCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTg                            <  4:67501/148‑1 (MQ=255)
                                                                                                               cGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCa                                                                               >  4:41433/1‑95 (MQ=255)
                                                                                                               cGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCa                                                                               <  3:41433/95‑1 (MQ=255)
                                                                                                                ggATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGACGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCggtctggtct                        >  4:248816/1‑149 (MQ=255)
                                                                                                                   tGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGa                     >  5:78042/1‑149 (MQ=255)
                                                                                                                     gCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGAc                    <  5:273896/148‑1 (MQ=255)
                                                                                                                      catcatCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACgg                  >  6:318695/1‑149 (MQ=255)
                                                                                                                       atcatcAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGc                 <  8:279919/149‑1 (MQ=255)
                                                                                                                          atcaGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCttt              <  6:290001/149‑1 (MQ=255)
                                                                                                                           tcaGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCtttt             <  2:348426/149‑1 (MQ=255)
                                                                                                                             aGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGTGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTgg           <  6:163207/149‑1 (MQ=255)
                                                                                                                               acccgctgCAATGTCGAATTTCGGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGcc         >  2:190694/8‑149 (MQ=255)
                                                                                                                                tACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCt        >  8:198330/1‑149 (MQ=255)
                                                                                                                                  cTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCt        <  5:318695/147‑1 (MQ=255)
                                                                                                                                  cTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGATTTTGGCCTgg      >  4:213252/1‑149 (MQ=255)
                                                                                                                                   tGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTgg      >  5:166016/1‑148 (MQ=255)
                                                                                                                                    ctgCAATGTCGAATTTCGGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTg       <  8:277919/144‑1 (MQ=255)
                                                                                                                                    ctgCAATGTCGAATTTCGGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTg       >  7:277919/3‑146 (MQ=255)
                                                                                                                                     aGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCGGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGt     >  3:83842/1‑147 (MQ=255)
                                                                                                                                     aGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAActct                                                                                       >  8:115468/1‑65 (MQ=255)
                                                                                                                                     aGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAActct                                                                                       <  7:115468/65‑1 (MQ=255)
                                                                                                                                     aGCAATGGCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGt     <  6:78042/147‑1 (MQ=255)
                                                                                                                                      gCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGTAAg  <  3:120988/149‑1 (MQ=255)
                                                                                                                                       cAATGTCGAATTTCGGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGccc                                                                  <  5:52833/84‑1 (MQ=38)
                                                                                                                                       cAATGTCGAATTTCGGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGccc                                                                  >  6:52833/1‑84 (MQ=38)
                                                                                                                                                  |                                                                                                                                         
ACCTGTTTCAGACCAAACAAAGGGATGGCGCATACGTCGTCGTGAATCTGAACCTGCGCCTTTTTCCACAAATCGAGTTGAGTCTGCGGATCAGCCTCGACCCTGGCTTTCCGGATGGCATCATCAGCTACTGAGCAATGTCCGAAATTCGACATTGCAGCGGGTGCGCCGATGGCAGAAGCCGAATCGTAAAACTCTGTCAGCCAGTAGTCGGCGCCCGGATATCGAGCAGCACCGTAAAACACGATCGCGCTCTGGTCTTGACGGCTTTTGGCCTGGTAAG  >  NC_002947/5059359‑5059641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: