Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,064,612 C→A 6.8% G129C (GGT→TGT)  PP_4461 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,064,6120CA 6.8% 214.4 / 3.8 69G129C (GGT→TGT) PP_4461MFS transporter
Reads supporting (aligned to +/- strand):  ref base C (32/32);  new base A (3/2);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

CAGGAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTT  >  NC_002947/5064465‑5064760
                                                                                                                                                      |                                                                                                                                                    
cagGAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATACCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCt                                                                                                                                                     >  1:347268/1‑149 (MQ=255)
   gAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTgcg                                                                                                                                                  <  8:123115/149‑1 (MQ=255)
         gCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGCGGTCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTgcggcg                                                                                                                                               >  1:89461/1‑148 (MQ=255)
         gCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAAt                                                                                                                                            >  2:31897/1‑149 (MQ=255)
           aaGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCGCAGGTGGCGAAtgg                                                                                                                                          <  6:222752/149‑3 (MQ=255)
            agagCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCt                                                                                                                                         <  5:45432/149‑1 (MQ=255)
             gagCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCtt                                                                                                                                        >  4:249267/1‑149 (MQ=255)
              agCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTg                                                                                                                                       <  2:47803/149‑1 (MQ=255)
              agCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTg                                                                                                                                       <  6:217410/149‑1 (MQ=255)
               gcgcCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTACCCCATTCGCCACCTGCGGCGAATCCTTgg                                                                                                                                      <  1:31897/149‑1 (MQ=255)
                cgcCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGga                                                                                                                                     <  3:249267/149‑1 (MQ=255)
                cgcCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGga                                                                                                                                     <  4:39740/149‑1 (MQ=255)
                   cGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGAc                                                                                                                                  <  4:26694/149‑1 (MQ=255)
                           ctaGCGAGGCCAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAAc                                                                                                                          <  1:333917/149‑1 (MQ=255)
                           ctaGCGAGGCCAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATAAAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCaa                                                                                                                           >  6:111117/1‑148 (MQ=255)
                              gCGAGGCCAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGga                                                                                                                                     <  6:37879/135‑1 (MQ=255)
                              gCGAGGCCAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGga                                                                                                                                     >  5:37879/1‑135 (MQ=255)
                                gAGGCCAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCAGCGGCGAATCCTTGGAGACAACTCAACATGa                                                                                                                      >  6:230398/1‑148 (MQ=255)
                                    ccAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGc                                                                                                                 <  2:168861/149‑1 (MQ=255)
                                     cAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGa                                                                                                                      <  5:343820/143‑1 (MQ=255)
                                     cAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGa                                                                                                                      >  6:343820/1‑143 (MQ=255)
                                     cAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATACCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCa                                                                                                                <  2:347268/149‑1 (MQ=255)
                                     cAGCAAAGCGCACCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGC‑GGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACATGCGGCGAATCAATGGAGACATCTAAACAAGACTAg                                                                                                                  >  4:276522/1‑146 (MQ=255)
                                            gcgcCCCCATAGGAGC‑‑TAACGCTCTGTAAACTTCAAAATAACACCCATACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTg                                                                                                          <  1:300296/149‑1 (MQ=255)
                                            gcgcCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGC‑GGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                        <  3:276522/149‑1 (MQ=255)
                                             cgcCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                        <  4:339314/149‑1 (MQ=255)
                                             cgcCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                        <  3:53595/149‑1 (MQ=255)
                                             cgcCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGa                                                                                                        <  4:97787/149‑1 (MQ=255)
                                                ccccAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGa                                                                                                                                              <  7:66599/108‑1 (MQ=255)
                                                ccccAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGa                                                                                                                                              >  8:66599/1‑108 (MQ=255)
                                                      aggagC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGga                                                                                                                                     <  8:3849/111‑1 (MQ=255)
                                                      aggagC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGga                                                                                                                                     >  7:3849/1‑111 (MQ=255)
                                                      aggagC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGAAATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCCACATGAGTACCAGAAATGGAGCACCAACg                                                                                               >  4:261263/1‑149 (MQ=255)
                                                         agC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAAt                                                                                                                                            >  3:55831/1‑101 (MQ=255)
                                                         agC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAAt                                                                                                                                            <  4:55831/101‑1 (MQ=255)
                                                                aCGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATa                                                                                         <  2:46866/147‑1 (MQ=255)
                                                                  gCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGtct                                                                                     <  1:147268/149‑1 (MQ=255)
                                                                          aaCTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATAAAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGt                                                                              <  5:111117/148‑1 (MQ=255)
                                                                                cAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGtct                                                                                     >  4:180143/1‑135 (MQ=255)
                                                                                cAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGtct                                                                                     <  3:180143/135‑1 (MQ=255)
                                                                                    gAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAAc                                                                                                >  7:289074/1‑120 (MQ=255)
                                                                                    gAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAAc                                                                                                <  8:289074/120‑1 (MQ=255)
                                                                                        tCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGaccaa                                                                >  2:87437/1‑149 (MQ=255)
                                                                                            ttACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGaccaatac                                                             >  7:75915/1‑148 (MQ=255)
                                                                                                  ccTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACt                                                      <  2:89461/149‑1 (MQ=255)
                                                                                                                   cACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGc                                        <  5:162712/146‑1 (MQ=255)
                                                                                                                   cACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGc                                        >  6:162712/1‑146 (MQ=255)
                                                                                                                   cACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCaaa                                     >  4:51608/1‑149 (MQ=255)
                                                                                                                    aCGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCaaa                                     >  6:320784/1‑148 (MQ=255)
                                                                                                                      gATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAaataa                                  >  1:275627/1‑149 (MQ=255)
                                                                                                                        tataAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATc                                <  2:275627/149‑1 (MQ=255)
                                                                                                                           aaGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTc                             >  4:275421/1‑149 (MQ=255)
                                                                                                                            aGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATc                                >  2:318157/1‑145 (MQ=255)
                                                                                                                            aGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATc                                <  1:318157/145‑1 (MQ=255)
                                                                                                                              aTGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTcag                           >  5:90936/1‑148 (MQ=255)
                                                                                                                               tGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGCCAACTCAACATGACAAGCAGAATAGGAGCACCCACGCCAATAGTCTCGTATGTTGGAATCAGCCCAATACCCATACTGCCGATTGCCATGCAAATACTCGTcagcc                         >  5:330843/1‑148 (MQ=255)
                                                                                                                                gCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGt                                                                                       >  3:272885/1‑86 (MQ=255)
                                                                                                                                gCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGt                                                                                       <  4:272885/86‑1 (MQ=255)
                                                                                                                                  agagGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAgcagca                      >  3:156534/1‑149 (MQ=255)
                                                                                                                                  agagGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGACATGCAAATAATCGTCAgcagca                      >  7:332931/1‑149 (MQ=255)
                                                                                                                                      tccaaggaTTCGCCGCAGGTGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATcgc                  >  6:51361/8‑149 (MQ=255)
                                                                                                                                        gCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGCCCAATACGAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCgg                >  2:21176/1‑149 (MQ=255)
                                                                                                                                        cccccATACGACACCTCCGCCGAAACCTTCCTCACATAACAACAAGACAAAAAAAATTTGAGAACCAACGCCATTTCTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATAGCAATGCAAATAATCGTAAGAAGCATCGCgg                >  2:15454/2‑149 (MQ=255)
                                                                                                                                         ccccATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCggg               >  6:340020/1‑149 (MQ=255)
                                                                                                                                         ccccATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCggg               >  5:179495/1‑149 (MQ=255)
                                                                                                                                         aaggaTTCGCCGCAGGTGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATc                                <  1:85430/128‑1 (MQ=255)
                                                                                                                                         aaggaTTCGCCGCAGGTGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATc                                >  2:85430/5‑132 (MQ=255)
                                                                                                                                          cccATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGc              <  5:230398/149‑1 (MQ=255)
                                                                                                                                                 gCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACAAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGcc       >  6:47569/1‑149 (MQ=255)
                                                                                                                                                      cTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCtt  >  6:175733/1‑149 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
CAGGAATGAGCAAGAGCGCCGAGGCTACTAGCGAGGCCAGCAAAGCGCCCCCAGAGGAGC‑‑TCACGCTCTGGAAACTTCCAAAGAA‑TCCCTTACGACCTGCAGGAGACCATTCCACGATATAAGATGCAGAGGTGCCCCATTCGCCACCTGCGGCGAATCCTTGGAGACATCTCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTT  >  NC_002947/5064465‑5064760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑