Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,105,066 A→T 7.5% intergenic (+89/+25) yhhS → / ← ydiJ carbohydrate efflux transporter/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,105,0660AT 7.5% 142.4 / 2.1 53intergenic (+89/+25)yhhS/ydiJcarbohydrate efflux transporter/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (25/24);  new base T (2/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

CTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGC‑CTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCGGTTGCCAGCGGCTCCCCGTCCTTGTC  >  NC_002947/5104924‑5105207
                                                                                                                                               |                                                                                                                                             
ctctATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCGCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAagcag                                                                                                                                          >  1:89542/1‑149 (MQ=255)
ctctATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGC‑CTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAagcagc                                                                                                                                         >  1:234631/1‑149 (MQ=255)
    aTTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGC‑CTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGccccc                                                                                                                                     >  5:224361/1‑149 (MQ=255)
     tttGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGC‑CTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCTTTGCAGcccttc                                                                                                                                    <  5:170208/149‑4 (MQ=255)
     tttGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGC‑CTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGccccc                                                                                                                                     <  2:234631/148‑1 (MQ=255)
        gTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGC‑CTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCaaa                                                                                                                                  <  2:235990/148‑1 (MQ=255)
           ccGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGC‑CTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGc                                                                                                                               <  2:47543/148‑1 (MQ=255)
              ccAAGGGCGTAGGGACAGGGACCGTACAACAGAAGC‑CTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTg                                                                                                                           <  2:100314/149‑1 (MQ=255)
                aaGGGCGTAGGGACAGGGACCGTACAACAGAAGC‑CTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGcc                                                                                                                                        >  4:300631/1‑134 (MQ=255)
                aaGGGCGTAGGGACAGGGACCGTACAACAGAAGC‑CTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGcc                                                                                                                                        <  3:300631/134‑1 (MQ=255)
                     cGTAGGGACAGGGACCGTACAACAGAAGC‑CTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCa                                                                                                                    <  2:107428/149‑1 (MQ=255)
                        agggacagggacCGTACAACAGAAGC‑CTGCAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCAGGATCCCCCGTAGGAGCGGGTTCCCCCGCGCAGGGCTGCAAAGCAGCCCCCAAAGGGCCTGAACCTCAgcg                                                                                                                 >  6:168252/1‑149 (MQ=39)
                                  acCGTACAACAGAAGC‑CTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTAc                                                                                                       >  4:30653/1‑149 (MQ=255)
                                  acCGTACAACAGAAGC‑CTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTAc                                                                                                       <  6:206067/149‑1 (MQ=255)
                                      tACAACAGAAGC‑CTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCa                                                                                                   >  4:259791/1‑149 (MQ=255)
                                                gC‑CTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGCAGGGCTGCAAAGCCGCCCCCCAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACaccaccg                                                                                          >  2:343951/1‑147 (MQ=255)
                                                 c‑cTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTgg                                                                                                             <  1:348737/128‑2 (MQ=255)
                                                 c‑cTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTgg                                                                                                             >  2:348737/1‑127 (MQ=255)
                                                    tGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGccccc                                                                                                                                     >  8:111627/1‑102 (MQ=255)
                                                    tGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGccccc                                                                                                                                     <  7:111627/102‑1 (MQ=255)
                                                                ccGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGc                                                                            <  3:259791/147‑1 (MQ=255)
                                                                ccGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCAt                                                                          >  4:350666/1‑149 (MQ=255)
                                                                    ccTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTc                                                                      <  6:224361/149‑1 (MQ=255)
                                                                    ccTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTc                                                                      <  3:22205/149‑1 (MQ=255)
                                                                      tctTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGGAGGAGCGGGTTGAGCCGCGCAGGGCTGGAAAGCAGCCCCCAAAGGCCCTGCACCTCAGCGCGGGGCGTAGTGCAACCACACCTGAAGCGGGTGACGCCTGTTcgg                                                                    >  4:163025/1‑147 (MQ=255)
                                                                          cgcgGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGc                                                                   <  3:30653/146‑1 (MQ=255)
                                                                           gcgGGTAACCCCGCCCCCCTGGCGTGCACGAGCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCgg                                                               >  5:176112/1‑149 (MQ=255)
                                                                               gTAACCCCGCCCCCCGGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCGGCACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCAt                                                           >  7:296622/1‑149 (MQ=255)
                                                                                    cccGCCCCCCTGGCGTGTACGATCCCCCGGAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCGGAACCTCAGCGCTGAGCGTACTGCACCACCACCGCAAGCGGGTGACGCATCTTCCGCTCGGTCAGGCGCt                                                      >  2:190353/1‑149 (MQ=38)
                                                                                      cGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTg                                                    <  1:343951/149‑1 (MQ=255)
                                                                                                gCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTAc                                          >  1:252615/1‑149 (MQ=255)
                                                                                                            ccccGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGa                                   >  5:211574/1‑144 (MQ=255)
                                                                                                            ccccGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGa                                   <  6:211574/144‑1 (MQ=255)
                                                                                                            ccccGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAAc                              <  2:89542/149‑1 (MQ=255)
                                                                                                             cccGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGGCCTGGCTACGGCACGAATAAcc                             >  4:51235/1‑149 (MQ=255)
                                                                                                                      gCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCGGTTGCCAg                    <  5:168252/149‑1 (MQ=255)
                                                                                                                      gCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCACAAAGGCCCTGAACCTCAGCGCAGAGCGTACTGCAACACCACCTCAACCGGGAGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACAGGAAGCCAg                    >  4:25400/1‑149 (MQ=255)
                                                                                                                       atgcttCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCACTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGTCCTGGCTACGGCACGAATAACCGGTTGCCAGc                   <  3:25400/145‑1 (MQ=255)
                                                                                                                         ggTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCGGTTGCCAGCgg                 <  3:350666/149‑1 (MQ=255)
                                                                                                                          ggttaCCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCGGTTGCCAGCGGc                <  5:287211/145‑1 (MQ=255)
                                                                                                                          ggttaCCCGCAAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACTCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCGGTTGCCAGCGGc                <  5:77220/145‑1 (MQ=255)
                                                                                                                          gTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGACTAACCGGTTGCCAGCGGc                >  6:74493/1‑149 (MQ=255)
                                                                                                                          gTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCGGtt                         <  1:350571/140‑1 (MQ=255)
                                                                                                                          gTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCGGtt                         >  2:350571/1‑140 (MQ=255)
                                                                                                                            tCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACAAACTCAAGCGGGTGCCGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATATCCGCTTGCCAGCGGCTc              >  5:273701/1‑149 (MQ=255)
                                                                                                                              aCCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCGGTTGCCAGCGGCTcc             <  6:176112/148‑1 (MQ=255)
                                                                                                                                ccGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACTCCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCt                                                <  1:83931/111‑1 (MQ=255)
                                                                                                                                ccGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCt                                                >  2:83931/1‑111 (MQ=255)
                                                                                                                                ccGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCGGTTGCCAGCGGCTCCCCg          >  7:20626/1‑149 (MQ=255)
                                                                                                                                 ctttgggggCTGCTTTGCAGCCCCCAAAGGCCCAGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGCTGACGCATCTTCCGCTCCGTCATGCGCTTGACCTGGCAACGGCAAGAATAACCTGTTGCCAGCGGCTCGCCGt         >  4:256062/7‑149 (MQ=255)
                                                                                                                                      ggggCTGCTTTGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCGGtt                         <  3:26275/127‑1 (MQ=255)
                                                                                                                                      ggggCTGCTTTGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCGGtt                         >  4:26275/2‑128 (MQ=255)
                                                                                                                                         gCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCGGTTGCCAGCGGCTCCCCGTCCTTGTc  >  5:199034/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                             
CTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGC‑CTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCGGTTGCCAGCGGCTCCCCGTCCTTGTC  >  NC_002947/5104924‑5105207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑