Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,118,197 A→G 12.5% intergenic (+29/‑30) PP_4504 → / → PP_4505 hypothetical protein/sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,118,1970AG12.5% 90.7 / 5.7 40intergenic (+29/‑30)PP_4504/PP_4505hypothetical protein/sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base A (15/20);  new base G (3/2);  total (18/22)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.00e-01

CTCACGGCTGACCAGCAGAAGAAGTTTGACGAACTGCAAAAAGAACGCGCCGCCAAGAAAGCCGAGTGGCAGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGTTCACTGTTCTGGCGCATCCTGGCCAGTTTCTGGCTGGCCATCACCCTGGTCGCAGGCCTGTCGATCCTGCTGGGGCACATGCTCAACCAGGACGCCTGGA  >  NC_002947/5118052‑5118338
                                                                                                                                                 |                                                                                                                                             
cTCACGGCTGACCAGCAGAAGAAGTTTGACGAACTGCAAAAAGAACGCGCCGCCAAGAAAGCCGAGTGGCAGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTg                                                                                                                                            >  8:262515/1‑149 (MQ=255)
   aCGGCTGACCAGCAGAAGAAGTTTGACGAACTGCAAAAAGAACGCGCCGCCAAGAAAGCCGAGTGGCAGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGcgg                                                                                                                                         <  2:253151/149‑1 (MQ=255)
   aCGGCTGACCAGCAGAAGAAGTTTGACGAACTGCAAAAAGAACGCGCCGCCAAGAAAGCCGAGTGGCAGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGcgg                                                                                                                                         >  4:168735/1‑149 (MQ=255)
      gCTGACCAGCAGAAGAAGTTTGACGAACTGCAAAAAGAACGCGCCGCCAAGAAAGCCGAGTGGCAGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGcgggcc                                                                                                                                      <  3:339826/149‑1 (MQ=255)
          aCCAGCAGAAGAAGTTTGACGAACTGCAAAAAGAACGCGCCGCCAAGAAAGCCGAGTGGCAGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGcgggccggg                                                                                                                                   <  7:262515/148‑1 (MQ=255)
            cagcagAAGAAGTTTGACGAACTGCAAAAAGAACGCGCCGCCAAGAAAGCCGAGTGGCAGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCAGTGATGcgggccgggcat                                                                                                                                <  5:234278/149‑3 (MQ=255)
                agaagaAGTTTGACGAACTGCAAAAAGAACGCGCCGCCAAGAAAGCCGAGTGGCAGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTcc                                                                                                                            <  2:318367/149‑1 (MQ=255)
                    gaagTTTGACGAACTGCAAAAAGAACGCGCCGCCAAGAAAGCCGAGTGGCAGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGtt                                                                                                                        >  2:110650/1‑149 (MQ=255)
                      agTTTGACGAACTGCAAAAAGAACGCGCCGCCAAGAAAGCCGAGTGGCAGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGttt                                                                                                                       <  6:141513/148‑1 (MQ=255)
                       gTTTGACGAACTGCAAAAAGAACGCGCCGCCAAGAAAGCCGAGTGGCAGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTag                                                                                                                     <  4:212703/149‑1 (MQ=255)
                          tGACGAACTGCAAAAAGAACGCGCCGCCAAGAAAGCCGAGTGGCAGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTagga                                                                                                                   <  1:110650/148‑1 (MQ=255)
                           gACGAACTGCAAAAAGAACGCGCCGCCAAGAAAGCCGAGTGGCAGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTaggag                                                                                                                  <  4:183981/148‑1 (MQ=255)
                              gAACTGCAAAAAGAACGCGCCGCCAAGAAAGCCGAGTGGCAGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTg                                                                                                               >  7:44508/1‑148 (MQ=255)
                                    cAAAAAGAACGCGCCGCCAAGAAAGCCGAGTGGCAGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGc                                                                                                        <  8:44508/149‑1 (MQ=255)
                                      aaaaGAACGCGCCGCCAAGAAAGCCGAGTGGCAGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTagga                                                                                                                   <  5:331388/136‑1 (MQ=255)
                                      aaaaGAACGCGCCGCCAAGAAAGCCGAGTGGCAGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTagga                                                                                                                   >  6:331388/1‑136 (MQ=255)
                                       aaaGAACGCGCCGCCAAGAAAGCCGAGTGGCAGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGtt                                                                                                     <  1:21538/149‑1 (MQ=255)
                                                 ccgccAAGAAAGCCGAGTGGCAGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGTTCACTGTTCTg                                                                                           <  8:168728/149‑1 (MQ=255)
                                                 ccgccAAGAAAGCCGAGTGGCAGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCAGTGATGcgggccgg                                                                                                                                    >  8:53511/1‑108 (MQ=255)
                                                                    gCAGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGTTCACTGTTCTGGCGCATCCTGGCCAGTTTc                                                                        >  5:190909/1‑149 (MQ=255)
                                                                      aGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGTTCACTGTTCTGGCGCATCCTGGCCAGTTTctg                                                                      >  8:146142/1‑149 (MQ=255)
                                                                         aGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGTTCACTGTTCTGGCGCATCCTGGCCAGTTTctggct                                                                   <  6:190909/149‑1 (MQ=255)
                                                                             ccAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGTTCACTGTTCt                                                                                            <  2:319513/120‑1 (MQ=255)
                                                                             ccAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGTTCACTGTTCt                                                                                            >  1:319513/1‑120 (MQ=255)
                                                                              cAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTTGGAGGTGCACTTGCGTTCACTGTTGTGGCGCATCCTGGGCAGGTTCTGGCTGGCCa                                                              >  8:315745/1‑149 (MQ=255)
                                                                               aGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGTTCACTGTTCTGGCGCATCCTGGCCAGTTTCTGGCTGGCCAt                                                             >  4:47258/1‑149 (MQ=255)
                                                                                            tGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGTTCACTGTTCTGGCGCATCCTGGCCAGTTTCTGGCTGGCCATCACCCTGGTCg                                                  >  8:259528/1‑147 (MQ=255)
                                                                                              aaaaaGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGTTCACTGTTCTGGCGCATCCTGGCCAGTTTCTGGCTGGCCATCACCCTGGTCGCAg                                               <  3:47258/148‑1 (MQ=255)
                                                                                                   ggCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGTTCACTGTTCTGGCGCATCCTGGCCAGTTTCTGGCTGGCCATCACCCTGGTCGCAGGCCTGt                                         <  7:259528/149‑1 (MQ=255)
                                                                                                           ggCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGTTCACTGTTCTGGCGCATCCTGGCCAGTTTCTGGCTGGCCATCACCCTGGTCGCAGGCCTGTCGATCctg                                 <  3:168735/149‑1 (MQ=255)
                                                                                                              ccAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGTTCACTGTTCTGGCGCATCCTGGCCAGTTTCTGGCTGGCCATCACCCTGGTc                                                   >  2:356418/1‑128 (MQ=255)
                                                                                                              ccAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGTTCACTGTTCTGGCGCATCCTGGCCAGTTTCTGGCTGGCCATCACCCTGGTc                                                   <  1:356418/128‑1 (MQ=255)
                                                                                                                      cTGTCCGCCACATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGAACTGGCGTTCACTGTTCTGGCGCATCCTGGCCAGTTTCTGGCTGGCCATCACCCTGGTCGAAGGCGTGTCGATCATGCTGGGGcagct                      >  2:236961/1‑146 (MQ=255)
                                                                                                                         tCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGTTCACTGTTCTGGCGCATCCTGGCCAGTTTCTGGCTGGCCATCAcc                                                         <  5:293985/111‑1 (MQ=255)
                                                                                                                         tCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGTTCACTGTTCTGGCGCATCCTGGCCAGTTTCTGGCTGGCCATCAcc                                                         >  6:293985/1‑111 (MQ=255)
                                                                                                                                  tGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGTTCACTGTTCTGGCGCATCCTGGCCAGTTTCTGGCTGGCCATCACCCTGGTCGCAGGCCTGTCGATCCTGCTGGGGCACATGCTCAACCAGGa          <  7:146142/149‑1 (MQ=255)
                                                                                                                                     ccggcccgCAGTGATGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGTTCACTGTTCTGGCGCATCCTGGCCAGTTTCTGGCTGGCCATCACCCTGGTCGCAGGCCTGTCGATCCTGCTGGGGCACATGCTCAACCAGGACGc       >  7:308278/1‑149 (MQ=255)
                                                                                                                                        gcccgCATCGCTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGTTCACTGTTCTGGCGCATCCTGGCCAGTTTCTGGCTGGCCATCACCCTGGTCGCAGGCCTGTCGATCCTGCTGGGGCACATGCTCaa                <  7:76807/137‑1 (MQ=255)
                                                                                                                                        gcccgCATCGCTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGTTCACTGTTCTGGCGCATCCTGGCCAGTTTCTGGCTGGCCATCACCCTGGTCGCAGGCCTGTCGATCCTGCTGGGGCACATGCTCaa                >  8:76807/1‑137 (MQ=255)
                                                                                                                                          ccgCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGTTCACTGTTCTGGCGCATCCTGGCCAGTTTCTGGCTGGCCATCACACTGGTCGCAGGCCTGTCTCTCATGCTGGGGCACATGCTCAACCAGGACGCCTGGa  >  4:234463/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
CTCACGGCTGACCAGCAGAAGAAGTTTGACGAACTGCAAAAAGAACGCGCCGCCAAGAAAGCCGAGTGGCAGGAGTTCCAGGCCTGGAAAGCTGAAAAAGGCGCCAAGGCCCAGTAAGCTGTCCGCCCCATGCCCGGCCCGCATCACTGCGGGCCGGGCTTTTCCCGTTTAGGAGGTGCACTTGCGTTCACTGTTCTGGCGCATCCTGGCCAGTTTCTGGCTGGCCATCACCCTGGTCGCAGGCCTGTCGATCCTGCTGGGGCACATGCTCAACCAGGACGCCTGGA  >  NC_002947/5118052‑5118338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑