Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,123,458 T→A 6.9% D225V (GAC→GTC)  PP_4510 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,123,4580TA 6.9% 169.5 / 2.0 58D225V (GAC→GTC) PP_4510hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (29/25);  new base A (2/2);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

CGGCCCCCTGCCCTCGAGCCTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCTTTCACCTTGTGCTTGAGGTACG  >  NC_002947/5123314‑5123605
                                                                                                                                                |                                                                                                                                                   
cggcCCCCTGCCCTCGAGCCTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGAAACAGCTCGACAGTGCCGTCCCATTGCTCGATCAGGTCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCAc                                                                                                                                                 <  6:292947/149‑1 (MQ=255)
    ccccTGCCCTCGAGCCTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTGGACTACCTc                                                                                                                                              >  1:328213/1‑148 (MQ=255)
      ccTGCCCTCGAGCCTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTcacc                                                                                                                                           >  8:190598/1‑149 (MQ=255)
      ccTGCCCTCGAGCCTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTcacc                                                                                                                                           <  3:241272/149‑1 (MQ=255)
          cccTCGAGCCTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTGGACTACCTcaattgc                                                                                                                                        <  2:328213/148‑6 (MQ=255)
             tCGAGCCTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGACGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTg                                                                                                                                    >  5:24916/1‑149 (MQ=255)
               gAGCCTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGc                                                                                                                                   <  7:190598/148‑1 (MQ=255)
                  ccTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGt                                                                                                                                                     <  1:217169/127‑1 (MQ=255)
                  ccTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGt                                                                                                                                                     >  2:217169/1‑127 (MQ=255)
                                gcgcAACGCCGCCACGGCCTTGGCTTCGGCCAGCCGATACATTTTGAAACTTCCGTCCCAATGCTTGTTCTTGGCCGTGCATGACTCCTCCCATTTCCCGCAATTTTTGTATTCCACCTCACCCACCTTGCGGATCTCGGCatgatg                                                                                                                   <  7:226020/147‑1 (MQ=255)
                                gcgcAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGa                                                                                                                 >  1:190137/1‑149 (MQ=255)
                                       gccgccACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCg                                                                                                                                  >  5:34379/1‑125 (MQ=255)
                                       gccgccACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCg                                                                                                                                  <  6:34379/125‑1 (MQ=255)
                                        ccgccACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCAAGATCAGGGCCGTGCACGACTACACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCACGGCATGATGGAAATGCCCg                                                                                                         >  1:97971/1‑149 (MQ=255)
                                                  cTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCAccc                                                                                               <  7:84295/149‑1 (MQ=255)
                                                                gCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCAcgg                                                                                 >  3:279038/1‑149 (MQ=255)
                                                                        aGCTCGACACTGCCGTCCCAATGCTCGATCTGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGa                                                                                                                 >  1:28654/1‑109 (MQ=255)
                                                                        aGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGa                                                                                                                 <  2:28654/109‑1 (MQ=255)
                                                                        aGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCa                                                                         <  2:645/149‑1 (MQ=255)
                                                                          cTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACt                                                                       >  7:118318/1‑149 (MQ=255)
                                                                                acTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCgg                                                                                                                                 <  6:14550/85‑1 (MQ=255)
                                                                                acTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCgg                                                                                                                                 >  5:14550/1‑85 (MQ=255)
                                                                                acTGACGTCCCAATGCGCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTCCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATgg                                                                  <  2:97971/148‑1 (MQ=255)
                                                                                  tGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGc                                                                >  1:37032/1‑148 (MQ=255)
                                                                                    ccGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCAc                                                                                                                                                 >  6:117083/1‑65 (MQ=255)
                                                                                    ccGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCAc                                                                                                                                                 <  5:117083/65‑1 (MQ=255)
                                                                                       tCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGc                                                          >  7:147758/1‑149 (MQ=255)
                                                                                           aaTGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTtc                                                      >  6:271792/1‑149 (MQ=255)
                                                                                             tGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTtctc                                                    <  5:271792/149‑1 (MQ=255)
                                                                                                tCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCAt                                                                    >  5:212909/1‑130 (MQ=255)
                                                                                                tCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCAt                                                                    <  6:212909/130‑1 (MQ=255)
                                                                                                tCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCg                                                   >  7:16191/1‑147 (MQ=255)
                                                                                                tCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATAAGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCAATCATGGCCAATGCCGTTCTCg                                                   >  6:355052/1‑147 (MQ=255)
                                                                                                  gctCAGCGCCGGGCACGACTCCACCCAGTCCCCCCAATTGCCGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCCTTCTCGaa                                                 <  4:38988/145‑1 (MQ=255)
                                                                                                      aGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCt                                           <  2:190137/149‑1 (MQ=255)
                                                                                                        ggCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTtc                                                      >  6:6965/1‑136 (MQ=255)
                                                                                                        ggCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTtc                                                      <  5:6965/136‑1 (MQ=255)
                                                                                                         gCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCtgat                                        <  8:147758/149‑1 (MQ=255)
                                                                                                           cGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGc                                            >  4:280044/1‑143 (MQ=255)
                                                                                                           cGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGc                                            <  3:280044/143‑1 (MQ=255)
                                                                                                               cACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACAGCGGGTGCACTCATGGGCAATGGCGTTCTCGa                                                  <  4:178145/133‑1 (MQ=255)
                                                                                                               cACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACAGCGGGTGCACTCATGGGCAATGGCGTTCTCGa                                                  >  3:178145/1‑133 (MQ=255)
                                                                                                                aCGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTc                                 >  8:146655/1‑149 (MQ=255)
                                                                                                                  gACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCAc                               >  5:91029/1‑149 (MQ=255)
                                                                                                                       cACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCtgatg                                       >  2:326516/1‑136 (MQ=255)
                                                                                                                       cACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCtgatg                                       <  1:326516/136‑1 (MQ=255)
                                                                                                                       cACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCgcgc                          <  5:355052/149‑1 (MQ=255)
                                                                                                                        aCCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGcc                         >  4:184763/1‑149 (MQ=255)
                                                                                                                        aCCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGcc                         >  3:247063/1‑149 (MQ=255)
                                                                                                                          ccAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCtt                       >  2:50472/1‑149 (MQ=255)
                                                                                                                              tCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCTTTCAc                   <  8:118318/149‑1 (MQ=255)
                                                                                                                                cccGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCTTTCAcc                  >  5:371336/1‑148 (MQ=255)
                                                                                                                                cccGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCTTTCAcc                  <  6:371336/148‑1 (MQ=255)
                                                                                                                                  cGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCTTTCACCTtg               <  4:279038/149‑1 (MQ=255)
                                                                                                                                     gggtGAGGTGGACTACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGc                                            <  2:248602/114‑1 (MQ=255)
                                                                                                                                     gggtGAGGTGGACTACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGc                                            >  1:248602/4‑117 (MQ=255)
                                                                                                                                      aTTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCTTTCACCTTGTGCt           >  8:188003/1‑149 (MQ=255)
                                                                                                                                       ttGAGGTAGTCCACCTCACCCATCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCAACCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTCATGAAGTTCACCGCGCCATTCACCTTGTGCtt          >  7:55751/1‑149 (MQ=255)
                                                                                                                                          aGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCTTTCACCTTGTGCTTGa        <  4:134469/148‑1 (MQ=255)
                                                                                                                                               gTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCTTTCACCTTGTGCTTGAGGTACg  >  2:165519/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CGGCCCCCTGCCCTCGAGCCTTAAGCCGGCTCGCGCAACGCCGCCACGGCCTGCGCTTCGGCCAGCCGATACAGCTCGACACTGCCGTCCCAATGCTCGATCAGGGCCGTGCACGACTCCACCCAGTCCCCGCAATTGAGGTAGTCCACCTCACCCACCTTGCGGATCTCGGCATGATGGATATGCCCGCACACCACCCCGTGAAAACCACGGCGGGTGCACTCATGGGCAATGGCGTTCTCGAAGTCGCTGATGAAGTTCACCGCGCCTTTCACCTTGTGCTTGAGGTACG  >  NC_002947/5123314‑5123605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑