Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 427,753 G→T 5.9% L16M (CTG→ATG)  PP_0350 ← ferrichrome‑iron receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947427,7530GT 5.9% 215.0 / 3.6 68L16M (CTG→ATG) PP_0350ferrichrome‑iron receptor
Reads supporting (aligned to +/- strand):  ref base G (32/32);  new base T (2/2);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

GATGGCCAAGGCAATGCCGGCCTGGCTGGCGATCTGGTTGAGGGTGGTGGCCAGTGGCGCGCTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGAAAAGTGACAGGGTGAATCTGAAAAATATTGAGAATTATTTAATGGGA  >  NC_002947/427607‑427900
                                                                                                                                                   |                                                                                                                                                   
gATGGCCAAGGCAATGCCGGCCTGGCTGGCGATCTGGTTGAGGGTGGTGGCCAGTGGCGCGCTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAggg                                                                                                                                                   >  5:202280/1‑149 (MQ=255)
     ccAAGGCAATGCCGGCCTGGCTGGCGATCTGGTTGAGGGTGGTGGCCAGTGGCGCGCT‑GGCAGGTTGTACGCGGCGCG‑CGTGGATTGCGGGGCGGCGGACCAGGCAGGGCAGGTCAGGGGGGCAGCCAGGCCCATGGCCAGGGccctgc                                                                                                                                             >  1:52539/1‑145 (MQ=255)
      cAAGGCAATGCCGGCCTGGCTGGCGATCTGGTTGAGGGTGGTGGCCAGTGGCGCGCTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCa                                                                                                                                             <  3:289369/149‑1 (MQ=255)
       aaGGCAATGCCGGCCTGGCTGGCGATCTGGTTGA‑GGTGGTGGCCAGTGGCGCGCTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAgg                                                                                                                                           >  7:43269/1‑149 (MQ=255)
           cAATGCCGGCCTGGCTGGCGATCTGGTTGAGGGGGGGGGCCAGTGGCGCGCTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTg                                                                                                                                        <  1:240206/149‑1 (MQ=255)
              tGCCGGCCTGGCTGGCGATCTGGTTGAGGGTGGTGGCCAGTGGCGCGCTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTgggg                                                                                                                                     <  6:202280/149‑1 (MQ=255)
                 cGGCCTGGCTGGCGATCTGGTTGAGGGTGGTGGCCAGTGGCGCGCTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGgcgc                                                                                                                                  <  2:298158/149‑1 (MQ=255)
                  ggCCTGGCTGGCGATCTGGTTGAGGGTGGTGGCCAGTGGCGCGCTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCa                                                                                                                                 <  2:66572/149‑1 (MQ=255)
                      tggctggcGATCTGGTTGAGGGGGGTGGCCAGTGGCGCGCTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGa                                                                                                                              >  7:227237/1‑148 (MQ=255)
                        gctggcGATCTGGTTGAGGGTGGTGGCCAGTGGCGCGCTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGTatat                                                                                                                           <  2:95370/149‑1 (MQ=255)
                        gctggcGATCTGGTTGAGGGTGGTGGCCAGTGGCGCGCTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGatat                                                                                                                           <  1:284049/149‑1 (MQ=255)
                          tggcGATCTGGTTGAGGGTGGTGGCCAGTGGCGCGCTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGa                                                                                                                         >  5:216468/1‑149 (MQ=255)
                                  tGGTTGAGGGTGGTGGCCAGTGGCGCGCTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACgg                                                                                                                  >  3:178609/1‑148 (MQ=255)
                                    gTTGAGGGTGGTGGCCAGTGGCGCGCTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTggg                                                                                                                                      >  1:6614/1‑126 (MQ=255)
                                    gTTGAGGGTGGTGGCCAGTGGCGCGCTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTggg                                                                                                                                      <  2:6614/126‑1 (MQ=255)
                                    gTTGAGGGTGGTGGCCAGTGGCGCGCTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCAc                                                                                                               <  4:178609/149‑1 (MQ=255)
                                        ctggtgggGGCCAGTGGCGCGCTGTGCATGTTGTCGGC‑GCGCGACGTGGCTTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCTGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATAAACGGCACggg                                                                                                            <  8:75185/146‑1 (MQ=255)
                                                   cAGTTGCGCGCTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGt                                                                                                >  5:251202/1‑149 (MQ=255)
                                                   cAGTGGCGCGCTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAAc                                                                                                                    <  4:135342/129‑1 (MQ=255)
                                                   cAGTGGCGCGCTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAAc                                                                                                                    >  3:135342/1‑129 (MQ=255)
                                                   cAGTGGCGCGCTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGt                                                                                                >  4:272041/1‑149 (MQ=255)
                                                     gTGGCGCGCTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCTACGGCACGGGACATGCGGCGGTTc                                                                                              <  8:227237/149‑1 (MQ=255)
                                                        gcgcgcTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCt                                                                                                                                         <  5:27833/103‑1 (MQ=255)
                                                        gcgcgcTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCt                                                                                                                                         >  6:27833/1‑103 (MQ=255)
                                                        gcgcgcTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTc                                                                                           <  6:216468/149‑1 (MQ=255)
                                                            gcTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACg                                                                                       >  4:275149/1‑149 (MQ=255)
                                                                ggCAGTTTGTAGGC‑GCGCTACGTTGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGt                                                                                    <  6:251202/148‑1 (MQ=255)
                                                                 gCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGt                                                                                    >  3:143018/1‑147 (MQ=255)
                                                                     gTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTc                                                                                           <  2:25443/136‑1 (MQ=255)
                                                                     gTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTc                                                                                           >  1:25443/1‑136 (MQ=255)
                                                                        gTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGtgcttg                                                                           >  3:159516/1‑149 (MQ=255)
                                                                                cgcgACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTc                                                                    <  4:159516/149‑1 (MQ=255)
                                                                                          aTTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCcgagc                                                          >  1:253751/1‑149 (MQ=255)
                                                                                          aTTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCcgagc                                                          <  3:272041/149‑1 (MQ=255)
                                                                                              cTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCcgagc                                                          >  4:158332/1‑145 (MQ=255)
                                                                                               tCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTtgctg                                                                        <  8:135952/130‑1 (MQ=255)
                                                                                               tCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTtgctg                                                                        >  7:135952/1‑130 (MQ=255)
                                                                                                    ggcgAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGa                                                 >  5:46188/1‑148 (MQ=255)
                                                                                                       gAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTaa                                                                                  >  8:49658/1‑112 (MQ=255)
                                                                                                       gAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGAAATGCGGCGGTTCCTCGACGGGTaa                                                                                  <  7:49658/112‑1 (MQ=255)
                                                                                                        aaCAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGAAAAGt                                            <  2:202044/149‑1 (MQ=255)
                                                                                                          cAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCcgagc                                                          >  4:62877/1‑133 (MQ=255)
                                                                                                          cAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCcgagc                                                          <  3:62877/133‑1 (MQ=255)
                                                                                                          cAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGAAAAGTGa                                          >  3:259790/1‑149 (MQ=255)
                                                                                                          cAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGAAAAGTGa                                          >  7:6685/1‑149 (MQ=255)
                                                                                                           aaGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGAAAAGTGa                                          >  3:94341/1‑148 (MQ=255)
                                                                                                             ggCAGGGCTGGTCAGCGGGGCAGCAAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCATCGACGGGTAAGTGCTTGATGCTTCCTTGCCGAGCGAGATATGAAAAGTTACAg                                       >  6:368067/1‑149 (MQ=255)
                                                                                                              gCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGAAAAGTGACAgg                                      >  2:327699/1‑149 (MQ=255)
                                                                                                                aGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGAAAAGTGACAGGGt                                    >  1:7773/1‑149 (MQ=255)
                                                                                                                  ggCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGAAAAGTGACAGGGTGa                                  <  4:94341/149‑1 (MQ=255)
                                                                                                                    cTGGTCAGCGGGACAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCgaga                                                      <  1:43699/127‑1 (MQ=255)
                                                                                                                    cTGGTCAGCGGGACAGCCAGGCCGATGGCCAGGGCCATCAGGCTCGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCgaga                                                      >  2:43699/1‑127 (MQ=255)
                                                                                                                       gTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGAAAAGTGACAGGGTGAATc                               <  3:158332/147‑1 (MQ=255)
                                                                                                                       gTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGAAAAGTGACAGGGTGAATCt                              >  3:107674/1‑148 (MQ=255)
                                                                                                                          aGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGAAAAGTGACAGGGTGAATCTGaaa                          <  3:275149/149‑1 (MQ=255)
                                                                                                                                  aGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCTGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGAAAAGTGACAGGGTGAATCTGAAAAATATTga                  <  5:368067/149‑1 (MQ=255)
                                                                                                                                   cccAGCCTGATGGCCCTGGCCATCGGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTCCCTTGCCGAGCGAGATCTGAAAAGTGACAGGGTGAATCTGAAAAATAtt                    >  2:274320/2‑146 (MQ=255)
                                                                                                                                   cccAGCCTGATGGCCCTGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGaaaa                                              >  3:160903/2‑120 (MQ=255)
                                                                                                                                   cccAGCCTGATGGCCCTGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGaaaa                                              <  4:160903/119‑1 (MQ=255)
                                                                                                                                    ccAGCCTGATGGCCCTGGCCATCGGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTCCCTTGCCGAGCGAGATCTGAAAAGTGACAGGGTGAATCTGAAAAATATTgaga                <  1:274320/149‑1 (MQ=255)
                                                                                                                                          cGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGAAAAGTGACAGGGTGAATCTGAAAAATATTGAGAattatt          <  4:259790/149‑1 (MQ=255)
                                                                                                                                          cGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGAAAAGTGACAGGGTGAATCTGAAAAATATTGAGAattatt          >  8:41163/1‑149 (MQ=255)
                                                                                                                                          cGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGAAAAGTGACAGGGTGAATCTGAAAAATATTGAGAattatt          >  2:333425/1‑149 (MQ=255)
                                                                                                                                          cGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGAAAAGTGACAGGGTGAATCTGAAAAATATTGAGAattatt          <  8:6685/149‑1 (MQ=255)
                                                                                                                                           gATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGAAAAGTGACAGGGTGAATCTGAAAAATATTGAGAATTAttt         <  2:253751/149‑1 (MQ=255)
                                                                                                                                             tGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGAAAAGTGACAGGGTGAATCTGAAAAATATTGAGAATTATTTaa       <  7:41163/149‑1 (MQ=255)
                                                                                                                                                ccAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGAAAAGTGACAGGGTGAATCTGAAAAATATTGAGAATTATTTAATgg    >  7:104630/1‑149 (MQ=255)
                                                                                                                                                  aGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGAAAAGTGACAGGGTGAATCTGAAAAATATTGAGAATTATTTAATGGGa  >  3:163430/1‑149 (MQ=255)
                                                                                                                                                  aGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGAAAAGTGACAGGGTGAATCTGAAAAATATTGAGAATTATTTAATGGGa  <  1:327699/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GATGGCCAAGGCAATGCCGGCCTGGCTGGCGATCTGGTTGAGGGTGGTGGCCAGTGGCGCGCTGGGCAGGTTGTAGGC‑GCGCGACGTGGATTGCTCGGCGGCGAACAAGGCAGGGCTGGTCAGCGGGGCAGCCAGGCCGATGGCCAGGGCCATCAGGCTGGGGCGCAGGATATGATCAACGGCACGGGACATGCGGCGGTTCCTCGACGGGTAAGTGCTTGCTGCTTCCTTGCCGAGCGAGATCTGAAAAGTGACAGGGTGAATCTGAAAAATATTGAGAATTATTTAATGGGA  >  NC_002947/427607‑427900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑