Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,172,520 T→C 7.4% H147R (CAC→CGC)  fadD‑I ← long‑chain‑fatty acid‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,172,5200TC 7.4% 150.6 / 2.1 54H147R (CAC→CGC) fadD‑Ilong‑chain‑fatty acid‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (24/26);  new base C (2/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

CCTTGCCCAGCGCCAGGGCGTCATTGAAACGCACGGCCTGCGGCAGGTTGTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCACCCGAGTCGTTGAACTGGTGTTCCATCTCCCGCGCGGTGTACAGCGGGTTGGTGTTGACCACGAT  >  NC_002947/5172374‑5172659
                                                                                                                                                  |                                                                                                                                           
ccTTGCCCAGCGCCAGGGCGTCATTGAAACGCACGGCCTGCGGCAGGTTGTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGc                                                                                                                                           <  5:222992/149‑1 (MQ=255)
ccTTGCCCAGCGCCAGGGCGTCATTGAAACGCACGGCCTGCGGCAGGTTGTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGc                                                                                                                                           >  7:148677/1‑149 (MQ=255)
 cTTGCCCAGCGCCAGGGCGTCATTGAAACGCACGGCCTGCGGCAGGTTGTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGcc                                                                                                                                          >  7:120004/1‑149 (MQ=255)
        aGCGCCAGGGCGTCATTGAAACGCACGGCCTGCGGCAGGTTGTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGt                                                                                                                                             <  7:103148/139‑1 (MQ=255)
        aGCGCCAGGGCGTCATTGAAACGCACGGCCTGCGGCAGGTTGTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGt                                                                                                                                             >  8:103148/1‑139 (MQ=255)
             cAGGGCGTCATTGAAACGCACGGCCTGCGGCAGGTTGTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGttt                                                                                                                              >  4:325554/1‑149 (MQ=255)
               gggCGTCATTGAAACGCACGGCCTGCGGCAGGTTGTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACTTGCCTGACCTGGGTTTTg                                                                                                                            <  5:86038/149‑1 (MQ=255)
               gggCGTCATTGAAACGCACGGCCTGCGGCAGGTTGTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTg                                                                                                                            <  8:120004/149‑1 (MQ=255)
                   gTCATTGAAACGCACGGCCTGCGGCAGGTTGTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGca                                                                                                                        <  8:207268/149‑1 (MQ=255)
                       ttGAAACGCACGGCCTGCGGCAGGTTGTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGcaccac                                                                                                                    >  6:132659/1‑149 (MQ=255)
                       ttGAAACGCACGGCCTGCGGCAGGTTGTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGcaccac                                                                                                                    >  6:86367/1‑149 (MQ=255)
                         gAAACGCACGGCCTGCGGCAGGTTGTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGCGCCTGACCTGGGTTTTGGGCACCACtt                                                                                                                  >  8:192760/1‑149 (MQ=255)
                          aaaCGCACGGCCTGCGGCAGGTTGTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACtt                                                                                                                  <  2:238317/148‑1 (MQ=255)
                          aaaCGCACGGCCAGCGGCAGGTTGTAGGCCGGCACCATCTTCTTCACGAACTAGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACttt                                                                                                                 >  2:356284/1‑149 (MQ=255)
                            aCGCACGGCCTGCGGCAGGTTGTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCGTGCCCTGGGTTGTGGGGACCACttttt                                                                                                               >  4:342013/1‑149 (MQ=255)
                            aCGCACGGCCTGCGGCAGGTTGTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACttttt                                                                                                               >  4:336620/1‑149 (MQ=255)
                              gCACGGCCTGCGGCAGGTTGTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGCGCCTGACCTGGGTTTTGGGCACCACTTTTTcc                                                                                                             >  7:78990/1‑149 (MQ=255)
                                  ggCCTGCGGCAGGTTGTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCa                                                                                                         <  1:149251/149‑1 (MQ=255)
                                   gCCTGCGGCAGGTTGTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAg                                                                                                        <  6:333575/149‑1 (MQ=255)
                                        cGGCAGGTTGTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTgg                                                                                                    <  3:325554/148‑1 (MQ=255)
                                              gTTGTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGt                                                                                             <  5:86367/149‑1 (MQ=255)
                                                 gTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTgg                                                                                          >  1:173130/1‑149 (MQ=255)
                                                 gTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTg                                                                                           <  4:202273/148‑1 (MQ=255)
                                                          cACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGcaca                                                                                 >  5:198770/1‑149 (MQ=255)
                                                              acttaattcACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAg                                                                             <  1:124409/143‑1 (MQ=255)
                                                                cttcttCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAggg                                                                           >  6:291491/1‑149 (MQ=255)
                                                                   cttcACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGc                                                                          <  3:342013/147‑1 (MQ=255)
                                                                   cttcACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGc                                                                          <  3:336620/147‑1 (MQ=255)
                                                                      cACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAgg                                                                            >  2:108940/1‑142 (MQ=255)
                                                                      cACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAgg                                                                            <  1:108940/142‑1 (MQ=255)
                                                                          tACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGGGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCAcc                                                                 <  8:148677/149‑1 (MQ=255)
                                                                            cTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGCGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCACCCg                                                               <  8:78990/149‑1 (MQ=255)
                                                                             ttGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCAccc                                                                >  4:6678/1‑147 (MQ=255)
                                                                                aTCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAgg                                                                                     <  1:209629/123‑1 (MQ=255)
                                                                                aTCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAgg                                                                                     >  2:209629/1‑123 (MQ=255)
                                                                                 tCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACGACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCACCCGAGTc                                                           <  1:356284/148‑1 (MQ=255)
                                                                                   aCGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCACCCGAGTCGtt                                                        <  5:138010/149‑1 (MQ=255)
                                                                                         ttGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCACCCGAGTCGTTGAACt                                                   <  4:109179/148‑1 (MQ=255)
                                                                                          tGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCAGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCACCCGAGTCGTTGAACTgg                                                 >  5:288509/1‑149 (MQ=255)
                                                                                            aTCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCACCCGAGTCGTTGAACTGgt                                                <  3:6678/148‑1 (MQ=255)
                                                                                            aTCAGCAGGCGCTTGAGGGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCACCCGAGTCGTTGAACTGgt                                                <  6:134466/148‑1 (MQ=255)
                                                                                                 cagGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCACCCGAGTCGTTGAACTGGTGTTCCa                                          >  6:136527/1‑149 (MQ=255)
                                                                                                            gtggtgGCAGCAGGTCGGCAACTTCAGTGACGATAACGCGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCACCCGAGTCGTTGAACTGGTGTTCCATCTCCcgcgcg                               <  7:192760/149‑1 (MQ=255)
                                                                                                               gtggCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCACCCGAGTCGTTGAACTGGTGTTCCATCTCCCGCGCGgtg                            >  7:245842/1‑149 (MQ=255)
                                                                                                               cgggCAGCAGGCCGGCAACTTCCGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCACCCGAGTCGTTGAACTGGTGTTCCATCTCCCGCGCGgt                             <  4:295113/146‑1 (MQ=255)
                                                                                                                  gcagcaGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCACCCGAGTCGTTGAACTGGTGTTCCATCTCCCGCGCGGTGTa                          >  2:313294/1‑148 (MQ=255)
                                                                                                                  gcagcaGGTCGGCAACTTCAGTGACGATAACGTGCCTCACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCACCCGAGTCGTTGAACTGGTGTTCCATCTCCCGCGCGGTGTa                          >  6:85036/1‑148 (MQ=255)
                                                                                                                                  ttCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCACCCGAGTCGTTGAACTGGTGTTCCATCTCCCGCGCGGTGTACAGCGGGTTGGTGTTGa         >  3:247203/1‑149 (MQ=255)
                                                                                                                                  ttCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCACCCGAGTCGTTGAACTGGTGTTCCATCTCCCGCGCGGTGTACAGCGGGTTGGTGTTGa         >  3:242465/1‑149 (MQ=255)
                                                                                                                                    cAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCACCCGAGTCGTTGAACTGGTGTTCCATCTCCCGCGCGGTGTACAGCGGGTTGGTGTTGa         <  5:136527/147‑1 (MQ=255)
                                                                                                                                    cAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCACCCGAGTCGTTGAACTGGTGTTCCATCTCCCGCGCGGTGTACAGCGGGTTGGTGTTGAc        <  8:245842/148‑1 (MQ=255)
                                                                                                                                      gTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCAccc                                                                <  2:242851/90‑1 (MQ=255)
                                                                                                                                      gTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCAccc                                                                >  1:242851/1‑90 (MQ=255)
                                                                                                                                         aCGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCacac                                                                                >  3:277810/1‑71 (MQ=255)
                                                                                                                                         aCGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCacac                                                                                <  4:277810/71‑1 (MQ=255)
                                                                                                                                          cGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCACCCGAGTCGTTGAACTGGTGTTCCATCTCCCGCGCGGTGTACAGCGGGTTGGTGTTGACCACGAt  >  1:19045/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                           
CCTTGCCCAGCGCCAGGGCGTCATTGAAACGCACGGCCTGCGGCAGGTTGTAGGCCGGCACCATCTTCTTCACGTACTTGATCACGCTGTTGATCAGCAGGCGCTTGAGTGGTGGCAGCAGGTCGGCAACTTCAGTGACGATAACGTGCCTGACCTGGGTTTTGGGCACCACTTTTTCCGCCAGGTGGGCCATGTTGGCCAGGCACACCAGGGCCTTGGCACCCGAGTCGTTGAACTGGTGTTCCATCTCCCGCGCGGTGTACAGCGGGTTGGTGTTGACCACGAT  >  NC_002947/5172374‑5172659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑