| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,173,251 | G→A | 9.8% | intergenic (‑292/+40) | fadD‑I ← / ← fadD‑II | long‑chain‑fatty acid‑‑CoA ligase/long‑chain‑fatty acid‑‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,173,251 | 0 | G | A | 9.8% | 131.6 / 4.8 | 51 | intergenic (‑292/+40) | fadD‑I/fadD‑II | long‑chain‑fatty acid‑‑CoA ligase/long‑chain‑fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base G (20/26); new base A (2/3); total (22/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.51e-01 | |||||||||||
ACAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCGGTGAAGTT > NC_002947/5173106‑5173395 | aCAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAgtgt < 2:123087/149‑1 (MQ=255)aCAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAgtgt > 4:345504/1‑149 (MQ=255)aCAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAgtgt < 5:243837/149‑1 (MQ=255) aTGGTCACAAAAATGACTCAATGAACAATACAGTTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACACTGCCCAATGTAGGAGCg < 5:98256/147‑1 (MQ=39) aTGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACACTGCCCAATGTAGGAGCgg < 6:47030/148‑1 (MQ=255) ggTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑Gtt > 1:147862/1‑149 (MQ=255) tCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTa > 1:320965/1‑149 (MQ=255) cacaAAAATGACTCAATGAACAATACAGGTCATTTTTAGATTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGGAGTGTAGGAGCGG‑GTTTAc > 8:133744/1‑149 (MQ=255) acaAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTAcc > 7:228300/1‑149 (MQ=255) caAAAATGAGTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCGTAGATTGGGGCGTGGAGGAGCGG‑GTTTAccc > 2:198104/1‑149 (MQ=255) caAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTAccc < 7:222882/149‑1 (MQ=255) cTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGc < 6:259537/130‑1 (MQ=255) cTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGc > 5:259537/1‑130 (MQ=255) cAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGc > 3:64680/1‑148 (MQ=255) aaTGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCa > 5:179614/1‑149 (MQ=255) aaCAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATccc > 5:297529/1‑149 (MQ=255) aCAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAgtgt > 1:174849/1‑116 (MQ=255) aCAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAgtgt < 2:174849/116‑1 (MQ=255) aCAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCg < 6:297529/149‑1 (MQ=255) cacaaccggTCATTTTTAGAGTAATTATCCAAAATGCCGCACATACGGCCGTATACACCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTCGCAGCGG‑GTTTACCCGCGAAGAAGCCATCCCgg < 4:134286/142‑1 (MQ=38) caaaccAGGTCATTTTTACACTAATTATCCAAAATGACGCAGATAAGGCTGTATACAGCCTGTTAGAGCGGTTTAAGCCATCCGCGGGT‑‑AACCCCGCTCCTACATTGGCCAGTGTAGGCGCGG‑GTTTACCCGCGAAGAAGCCATCCCgg < 4:262751/144‑1 (MQ=255) tACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑t > 5:106571/1‑149 (MQ=255) cattAATTATCCAAAAGCTCGCAGATCAGGCTCTATAGAGCCTGTTAGACCGGTTAAAGGCATTCGCGGGT‑‑AAACCCGCGCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATcgcg < 8:228300/145‑1 (MQ=38) aTTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTc > 5:17271/1‑149 (MQ=255) ccAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCccgcc < 3:345504/149‑1 (MQ=255) aaaaTGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAAACCCCAGCTACATTGGGGAGTGTAGGAGCGG‑GGTTTCCCGCGAAGAAGCCATCCCGGTT‑TAACGCGATATAGCGCAACTCCcgccgc > 6:238981/1‑149 (MQ=255) tCGCAGATAAGGCTGTATAGAGCGTGTTAGATCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATc < 3:273689/149‑1 (MQ=255) gCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCtt < 1:198104/149‑1 (MQ=255) cAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTATTCACGCTATATCGCGCAACTCCCGCCGCAAAATCtt < 1:53106/149‑1 (MQ=255) cAGATAAGGCGGTATAGAGCCTGTTAGAGCGGTTAAAGGCATTCGCGGGT‑‑AAACCGGCTCATACATTGCGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTg < 7:133744/149‑1 (MQ=39) cTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGc < 6:17271/149‑1 (MQ=255) agagCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATg > 8:57090/1‑149 (MQ=255) gagCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATg < 6:106571/148‑1 (MQ=255) agCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATggg > 5:362974/1‑149 (MQ=255) agtGTTAGAGAGGTTTTAGTGTTTCGCGGGTTAAAACTCCTTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCTAAATCTTGCCCACCGGCGTCATgg < 1:300147/146‑1 (MQ=255) gTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAAt < 5:284027/123‑1 (MQ=255) gTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAAt > 6:284027/1‑123 (MQ=255) agCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTcg > 5:281569/1‑149 (MQ=255) gCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTcgc < 7:175279/149‑1 (MQ=255) cGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTcgcg < 2:147862/149‑1 (MQ=255) gTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTcgcg > 7:257158/1‑147 (MQ=255) aaGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTcgc > 7:109380/1‑142 (MQ=255) aaGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTcgc < 8:109380/142‑1 (MQ=255) gccATCCGCGGTA‑‑AAACCCGCTCCTACATTGGCAAGTGACGTAGGGG‑TTTAACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGa < 5:238981/147‑1 (MQ=255) cgGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTg < 5:280239/149‑1 (MQ=255) gggT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTgg < 2:320965/149‑1 (MQ=255) t‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTa > 1:43782/1‑149 (MQ=255) aCCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACtt < 8:257158/149‑1 (MQ=255) cccGCTCCTACACTGCCCAATGTAGGAGCGG‑GTTTACCCGCGAATAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATgg < 6:54831/112‑1 (MQ=37) cccGCTCCTACACTGCCCAATGTAGGAGCGG‑GTTTACCCGCGAATAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATgg > 5:54831/1‑112 (MQ=37) ccGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTg < 7:57090/149‑1 (MQ=255) gCTCCTACACTGCCCAATGTAGGAGCGGAGTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGt > 1:22715/1‑149 (MQ=39) atcATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCgg < 5:327608/147‑1 (MQ=255) ggCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCGGTGAAGtt < 6:179614/149‑1 (MQ=255) aGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGAtg > 8:116717/1‑117 (MQ=255) aGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGAtg < 7:116717/117‑1 (MQ=255) | ACAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAGTAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGT‑‑AAACCCGCTCCTACATTGGGCAGTGTAGGAGCGG‑GTTTACCCGCGAAGAAGCCATCCCGGTA‑TCACGCTATATCGCGCAACTCCCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCGGTGAAGTT > NC_002947/5173106‑5173395 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |