Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,201,440 G→C 7.4% F25L (TTC→TTG)  PP_4582 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,201,4400GC 7.4% 170.4 / 4.6 54F25L (TTC→TTG) PP_4582membrane protein
Reads supporting (aligned to +/- strand):  ref base G (24/26);  new base C (2/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

CTTGAAAGCGACATCCAGCGCAGTGATGGCGATTGCAGGGGCGAAGCTGTCCTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTT  >  NC_002947/5201293‑5201579
                                                                                                                                                   |                                                                                                                                           
cTTGAAAGCGACATCCAGCGCAGTGATGGCGATTGCAGGGGCGAAGCTGTCCTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGa                                                                                                                                            <  8:42434/149‑1 (MQ=255)
cTTGAAAGCGACATCCAGCGCAGTGATGGCGATTGCAGGGGAGAAGCTGTCCTTGACAGGGTCGACGATGGTCGGGGTCAGAAGCAGCACCGCAAGCACCACGCGTAGCGGTTCGCGCAGCCATCGCCACATCCAGCCGGTGAGCTTg                                                                                                                                             >  5:189401/1‑148 (MQ=255)
  tGAAAGCGACATCCAGCGCAGTGATGGCGATTGCAGGGGCGAAGCTGTCCTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAg                                                                                                                                          >  7:196578/1‑149 (MQ=255)
   gAAAGCGACATCCAGCGCAGTGATGGCGATTGCAGGGGCGAAGCTGTCCTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGaa                                                                                                                                           <  2:104932/147‑1 (MQ=255)
    aaaGCGACATCCAGCGCAGTGATGGCGATTGCAGGGGCGAAGCTGTCCTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGcc                                                                                                                                        <  8:196578/149‑1 (MQ=255)
           cATCCAGCGCAGTGATGGCGATTGCAGGGGCGAAGCTGTCCTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACcagc                                                                                                                                 <  5:111260/149‑1 (MQ=255)
                            gCGATTGCAGGGGCGAAGCTGTCCTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAggcggc                                                                                                                >  2:21458/1‑149 (MQ=255)
                              gATTGCAGGGGCGAAGCTGTCCTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTa                                                                                                              <  4:91891/149‑1 (MQ=255)
                                   cAGGGGCGAAGCTGTCCTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCg                                                                                                         <  3:186331/149‑1 (MQ=255)
                                    aGGGGCGAAGCTGTCCTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCg                                                                                                         >  4:128072/1‑148 (MQ=255)
                                      gggCGAAGCTGTCCTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACcagc                                                                                                                                 <  8:240988/122‑1 (MQ=255)
                                      gggCGAAGCTGTCCTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACcagc                                                                                                                                 >  7:240988/1‑122 (MQ=255)
                                        gCGAAGCTGTCCTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACaa                                                                                                    <  3:128072/149‑1 (MQ=255)
                                               tGTCCTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAg                                                                                             >  3:52499/1‑149 (MQ=255)
                                                 tCCTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAgg                                                                                            <  2:16801/148‑1 (MQ=255)
                                                  ccTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGcc                                                                                                 <  2:328458/142‑1 (MQ=255)
                                                  ccTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGcc                                                                                                 >  1:328458/1‑142 (MQ=255)
                                                            gTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGt                                                                                <  4:52499/149‑1 (MQ=255)
                                                                acgaTGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGAAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGtt                                                                            >  5:306011/1‑149 (MQ=255)
                                                                  gaTGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTtct                                                                          <  7:206014/149‑1 (MQ=255)
                                                                     ggTCGGGGTCAGCAGCAGCACCGCCAGCACCACTCGTATCGGTTCGCGCAGCCAGCGCCACATCCATCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCg                                                                        <  6:189401/148‑1 (MQ=255)
                                                                      gTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCTGTTCGCGCATCCAGAGCCACATCCAGCCGGTGAGCTTGAAGCCCACGAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCGCCAGGCGAAGGTGTAGTCGTTCTggg                                                                       <  6:306011/148‑1 (MQ=255)
                                                                               agcagcagcACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGtt                                                             >  7:46546/1‑149 (MQ=255)
                                                                                gcagcagcaCCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAg                                                                                       <  4:105390/122‑1 (MQ=255)
                                                                                gcagcagcaCCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAg                                                                                       >  3:105390/1‑122 (MQ=255)
                                                                                 cagcagcaCCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCg                                                           >  7:41560/1‑149 (MQ=255)
                                                                                    cagcaCCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAgg                                                                                                                    >  5:99078/1‑89 (MQ=255)
                                                                                    cagcaCCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAgg                                                                                                                    <  6:99078/89‑1 (MQ=255)
                                                                                      gcaCCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGc                                                        >  1:152349/1‑147 (MQ=255)
                                                                                            ccAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAgg                                                >  5:324395/1‑149 (MQ=255)
                                                                                             cAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAgg                                                >  6:206396/1‑148 (MQ=255)
                                                                                             cAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAgg                                                >  6:211524/1‑148 (MQ=255)
                                                                                                  ccacGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCaaa                                            <  5:305501/147‑1 (MQ=255)
                                                                                                  ccacGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCaaa                                            >  3:41155/1‑147 (MQ=255)
                                                                                                  ccacGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCaaa                                            >  6:305501/1‑147 (MQ=255)
                                                                                                       cgTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGc                                     <  4:41155/149‑1 (MQ=255)
                                                                                                         tAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCt                                   <  8:46546/149‑1 (MQ=255)
                                                                                                          aGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAg                                                                                 <  1:236933/102‑1 (MQ=255)
                                                                                                          aGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAg                                                                                 >  2:236933/1‑102 (MQ=255)
                                                                                                           gCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTAt                                 <  5:211524/149‑1 (MQ=255)
                                                                                                           gCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTAt                                 <  5:206396/149‑1 (MQ=255)
                                                                                                            cGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACt                                                                                                            >  6:366867/1‑73 (MQ=255)
                                                                                                            cGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACt                                                                                                            <  5:366867/73‑1 (MQ=255)
                                                                                                            cGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATg                                <  8:103817/149‑1 (MQ=255)
                                                                                                                  gcgcAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACAGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATaaa                           >  6:344715/1‑148 (MQ=255)
                                                                                                                          agcGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTg                                                         <  5:318803/110‑1 (MQ=255)
                                                                                                                          agcGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTg                                                         >  6:318803/1‑110 (MQ=255)
                                                                                                                           gcgcCACATCCAGCCGGGGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCg                 <  8:46628/149‑1 (MQ=255)
                                                                                                                                acaTCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGgcg             >  5:344281/1‑148 (MQ=255)
                                                                                                                                   tCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGa         >  2:295618/1‑149 (MQ=255)
                                                                                                                                     ctgctggTGGGCTTCAAGCTCACCGGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCa           <  4:337183/140‑1 (MQ=255)
                                                                                                                                     ctgctggTGGGCTTCAAGCTCACCGGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCa           >  3:337183/6‑145 (MQ=255)
                                                                                                                                         tggTGGGCTTCAAGCTCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCa                                                                    >  7:303581/2‑84 (MQ=255)
                                                                                                                                         tggTGGGCTTCAAGCTCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCa                                                                    <  8:303581/83‑1 (MQ=255)
                                                                                                                                          ggTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGtt  >  8:224891/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
CTTGAAAGCGACATCCAGCGCAGTGATGGCGATTGCAGGGGCGAAGCTGTCCTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTT  >  NC_002947/5201293‑5201579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑