Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,239,884 G→A 10.0% A144V (GCG→GTG)  ldh ← leucine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,239,8840GA10.0% 132.3 / 4.8 50A144V (GCG→GTG) ldhleucine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (23/22);  new base A (3/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-02

TCCGCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGCCTC  >  NC_002947/5239740‑5240032
                                                                                                                                                |                                                                                                                                                     
tCCGCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCAtg                                                                                                                                                   >  4:99004/1‑149 (MQ=255)
tCCGCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCAtg                                                                                                                                                   >  4:94015/1‑149 (MQ=255)
 ccGCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCAtgt                                                                                                                                                  >  7:291251/1‑149 (MQ=255)
  cgcgCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCAtgt                                                                                                                                                  <  3:178745/148‑1 (MQ=255)
       ccACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCACGAACACCCCCATCGCCACATGCGGCGAt                                                                                                                                            <  5:157143/149‑1 (MQ=255)
            gCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTc                                                                                                                                       <  3:99004/149‑1 (MQ=255)
            gCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTc                                                                                                                                       <  3:94015/149‑1 (MQ=255)
             cATGCAATTTCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTc                                                                                                                                       <  3:300948/148‑1 (MQ=255)
             cATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTc                                                                                                                                       >  4:300948/1‑148 (MQ=255)
             cATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCg                                                                                                                                      >  4:276023/1‑149 (MQ=255)
                 cAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGTCCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCg                                                                                                                                  <  2:133060/149‑1 (MQ=255)
                  aaTTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGa                                                                                                                                 >  8:138832/1‑149 (MQ=255)
                      gCTCCGCCAGGGCATAACCAACATTGCCCACCCCCTGGACCGCTACCCGCACGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACGCCCCCAGCGCCGCATGTGGCGATGGGTCGCCCGA‑TGc                                                                                                                             <  6:101240/148‑1 (MQ=255)
                          cGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAgtgg                                                                                                                        >  4:156064/1‑149 (MQ=255)
                            ccAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAgtggtg                                                                                                                      <  7:184584/149‑1 (MQ=255)
                            ccAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAgtggtg                                                                                                                      >  8:184584/1‑149 (MQ=255)
                             cAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGc                                                                                                                     >  8:263955/1‑149 (MQ=255)
                               gggCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTg                                                                                                                   <  3:156064/149‑1 (MQ=255)
                                    tAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGacac                                                                                                              >  1:247747/1‑149 (MQ=255)
                                           cATTGCCCAACTCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGAt                                                                                                                                            >  1:177735/1‑113 (MQ=255)
                                           cATTGCCCAACTCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGAt                                                                                                                                            <  2:177735/113‑1 (MQ=255)
                                                cccAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCt                                                                                                  >  4:123703/1‑149 (MQ=255)
                                                    aCCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTgg                                                                                              <  8:291251/149‑1 (MQ=255)
                                                     ccccTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGa                                                                                                                                             <  4:55696/102‑1 (MQ=255)
                                                     ccccTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGa                                                                                                                                             >  3:55696/1‑102 (MQ=255)
                                                           gACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGTGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATg                                                                                        <  8:306677/148‑1 (MQ=255)
                                                           gACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAAt                                                                                         <  7:263955/147‑1 (MQ=255)
                                                            cccccaaCCCCCCAGCCGCACAGGTCATCGCGGCCCAGCCGGACCGCGGTTGGGGCAAGTAGGCCCGCCAACCCCCCCAGCGCCGCATTGGTCGTTGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCa                                                                                      <  2:352842/143‑1 (MQ=255)
                                                            aCCGCTCCCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCa                                                                                      <  4:72240/149‑1 (MQ=255)
                                                                cTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAgg                                                                                                         <  3:116945/126‑1 (MQ=255)
                                                                cTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAgg                                                                                                         >  4:116945/1‑126 (MQ=255)
                                                                cTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCACATGCGGCGATgggg                                                                                                                                        >  2:57273/1‑95 (MQ=255)
                                                                cTACCCGCAAGCCTCTCAGGGCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCACATGCGGCGATgggg                                                                                                                                        <  1:57273/96‑2 (MQ=39)
                                                                      gCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTc                                                                             >  6:35835/1‑148 (MQ=255)
                                                                          gCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAgg                                                                        >  5:179474/1‑149 (MQ=255)
                                                                                 aGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGGGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGt                                                                 <  7:232500/149‑1 (MQ=255)
                                                                                                aGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGc                                                  <  6:179474/149‑1 (MQ=255)
                                                                                                    ggAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCga                                                >  8:268315/1‑147 (MQ=255)
                                                                                                    ggAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCga                                                <  7:268315/147‑1 (MQ=255)
                                                                                                     gAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCgatga                                             >  3:7928/1‑149 (MQ=255)
                                                                                                      aaCGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGACGAGGTGCCACTGTCCACGGCgatgat                                            >  1:46345/1‑149 (MQ=255)
                                                                                                           ggTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAgc                                       <  3:276023/149‑1 (MQ=255)
                                                                                                                    aCGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGc                               >  5:160213/1‑148 (MQ=255)
                                                                                                                           cccGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTACTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATc                       >  7:317335/1‑149 (MQ=255)
                                                                                                                            ccGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATc                       >  4:301261/1‑148 (MQ=255)
                                                                                                                                  acacCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATTGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCga                                                >  1:245608/1‑118 (MQ=255)
                                                                                                                                  acacCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCga                                                <  2:245608/117‑1 (MQ=255)
                                                                                                                                     gacccATCGCCACATGCGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCgg             >  3:233485/3‑149 (MQ=255)
                                                                                                                                     cccccATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCgg             <  5:35835/149‑1 (MQ=255)
                                                                                                                                          aTCGCCACATGCGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCa          >  6:356/1‑147 (MQ=255)
                                                                                                                                              ccgcATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTTGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGcc    <  7:138832/149‑1 (MQ=255)
                                                                                                                                                gcATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAg       <  6:55939/144‑1 (MQ=255)
                                                                                                                                                gcATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAg       >  5:55939/1‑144 (MQ=255)
                                                                                                                                                gcATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTACTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGCCTc  <  8:317335/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
TCCGCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGCCTC  >  NC_002947/5239740‑5240032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑