Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,239,889 T→C 10.4% P142P (CCA→CCG)  ldh ← leucine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,239,8890TC10.4% 119.8 / 5.5 50P142P (CCA→CCG) ldhleucine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (21/22);  new base C (3/2);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01

CCGCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGCCTCGAA  >  NC_002947/5239741‑5240035
                                                                                                                                                    |                                                                                                                                                   
ccGCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCAtgt                                                                                                                                                     >  7:291251/1‑149 (MQ=255)
 cgcgCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCAtgt                                                                                                                                                     <  3:178745/148‑1 (MQ=255)
      ccACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCACGAACACCCCCATCGCCACATGCGGCGAt                                                                                                                                               <  5:157143/149‑1 (MQ=255)
           gCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTc                                                                                                                                          <  3:99004/149‑1 (MQ=255)
           gCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTc                                                                                                                                          <  3:94015/149‑1 (MQ=255)
            cATGCAATTTCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTc                                                                                                                                          <  3:300948/148‑1 (MQ=255)
            cATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTc                                                                                                                                          >  4:300948/1‑148 (MQ=255)
            cATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCg                                                                                                                                         >  4:276023/1‑149 (MQ=255)
                cAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGTCCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCg                                                                                                                                     <  2:133060/149‑1 (MQ=255)
                 aaTTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGa                                                                                                                                    >  8:138832/1‑149 (MQ=255)
                     gCTCCGCCAGGGCATAACCAACATTGCCCACCCCCTGGACCGCTACCCGCACGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACGCCCCCAGCGCCGCATGTGGCGATGGGTCGCCCGA‑TGc                                                                                                                                <  6:101240/148‑1 (MQ=255)
                         cGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAgtgg                                                                                                                           >  4:156064/1‑149 (MQ=255)
                           ccAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAgtggtg                                                                                                                         <  7:184584/149‑1 (MQ=255)
                           ccAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAgtggtg                                                                                                                         >  8:184584/1‑149 (MQ=255)
                            cAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGc                                                                                                                        >  8:263955/1‑149 (MQ=255)
                              gggCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTg                                                                                                                      <  3:156064/149‑1 (MQ=255)
                                   tAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGacac                                                                                                                 >  1:247747/1‑149 (MQ=255)
                                          cATTGCCCAACTCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGAt                                                                                                                                               >  1:177735/1‑113 (MQ=255)
                                          cATTGCCCAACTCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGAt                                                                                                                                               <  2:177735/113‑1 (MQ=255)
                                               cccAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCt                                                                                                     >  4:123703/1‑149 (MQ=255)
                                                   aCCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTgg                                                                                                 <  8:291251/149‑1 (MQ=255)
                                                    ccccTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGa                                                                                                                                                >  3:55696/1‑102 (MQ=255)
                                                    ccccTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGa                                                                                                                                                <  4:55696/102‑1 (MQ=255)
                                                          gACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGTGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATg                                                                                           <  8:306677/148‑1 (MQ=255)
                                                          gACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAAt                                                                                            <  7:263955/147‑1 (MQ=255)
                                                           cccccaaCCCCCCAGCCGCACAGGTCATCGCGGCCCAGCCGGACCGCGGTTGGGGCAAGTAGGCCCGCCAACCCCCCCAGCGCCGCATTGGTCGTTGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCa                                                                                         <  2:352842/143‑1 (MQ=255)
                                                           aCCGCTCCCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCa                                                                                         <  4:72240/149‑1 (MQ=255)
                                                               cTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAgg                                                                                                            >  4:116945/1‑126 (MQ=255)
                                                               cTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAgg                                                                                                            <  3:116945/126‑1 (MQ=255)
                                                               cTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCACATGCGGCGATgggg                                                                                                                                           >  2:57273/1‑95 (MQ=255)
                                                               cTACCCGCAAGCCTCTCAGGGCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCACATGCGGCGATgggg                                                                                                                                           <  1:57273/96‑2 (MQ=39)
                                                                     gCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTc                                                                                >  6:35835/1‑148 (MQ=255)
                                                                         gCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAgg                                                                           >  5:179474/1‑149 (MQ=255)
                                                                                aGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGGGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGt                                                                    <  7:232500/149‑1 (MQ=255)
                                                                                               aGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGc                                                     <  6:179474/149‑1 (MQ=255)
                                                                                                   ggAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCga                                                   >  8:268315/1‑147 (MQ=255)
                                                                                                   ggAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCga                                                   <  7:268315/147‑1 (MQ=255)
                                                                                                    gAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCgatga                                                >  3:7928/1‑149 (MQ=255)
                                                                                                     aaCGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGACGAGGTGCCACTGTCCACGGCgatgat                                               >  1:46345/1‑149 (MQ=255)
                                                                                                          ggTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAgc                                          <  3:276023/149‑1 (MQ=255)
                                                                                                                   aCGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGc                                  >  5:160213/1‑148 (MQ=255)
                                                                                                                          cccGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTACTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATc                          >  7:317335/1‑149 (MQ=255)
                                                                                                                           ccGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATc                          >  4:301261/1‑148 (MQ=255)
                                                                                                                                 acacCCCCATCGCCGCATGTGGCGATGGGTCGCCCGATTGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCga                                                   >  1:245608/1‑118 (MQ=255)
                                                                                                                                 acacCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCga                                                   <  2:245608/117‑1 (MQ=255)
                                                                                                                                    gacccATCGCCACATGCGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCgg                >  3:233485/3‑149 (MQ=255)
                                                                                                                                    cccccATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCgg                <  5:35835/149‑1 (MQ=255)
                                                                                                                                         aTCGCCACATGCGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCa             >  6:356/1‑147 (MQ=255)
                                                                                                                                             ccgcATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTTGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGcc       <  7:138832/149‑1 (MQ=255)
                                                                                                                                               gcATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAg          <  6:55939/144‑1 (MQ=255)
                                                                                                                                               gcATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAg          >  5:55939/1‑144 (MQ=255)
                                                                                                                                               gcATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTACTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGCCTc     <  8:317335/149‑1 (MQ=255)
                                                                                                                                                   gtgGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGCCTCGaa  >  8:39401/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCGCGCCCACCGCATGCAATTGCTCCGCCAGGGCATAACCAACATTGCCCAACCCCTGGACCGCTACCCGCAAGCCTCTCAGGTCATCGCTGCCCAGCCGGAACGAGGTTGTGGCACGTATGCCCGCGAACACCCCCATCGCCGCATGTGGCGATGGGTCGCCCGA‑TGCAGTGGTGCTGGTCACGTAGGGCGTGCTGTGGGCAATGCAGTCCATGTCCAAGGTCGAGGTGCCACTGTCCACGGCGATGATGAAGCGCCCATGCAAGGTATCGATGAAGCGGCCAAAGGCCTCGAA  >  NC_002947/5239741‑5240035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑