Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,263,966	A→G	8.6%	V94A (GTG→GCG)	zinU ←	P‑loop guanosine triphosphatase‑dependent zinc chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,263,966	0	A	G	8.6%	158.2 / 5.0	59	V94A (GTG→GCG)	zinU	P‑loop guanosine triphosphatase‑dependent zinc chaperone
Reads supporting (aligned to +/- strand):  ref base A (24/29);  new base G (3/2);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

CGATGGTCTGGGTCAGGTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCCAGCAGTTGGCTG  >  NC_002947/5263820‑5264112                                                                                                                                                   |                                                                                                                                                     cGATGGTCTGGGTCAGGTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCAcc                                                                                                                                                    <  5:69337/149‑1 (MQ=255)            gTCAGGTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGaa                                                                                                                                         <  1:172330/149‑1 (MQ=255)                 gTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAg                                                                                                                                        >  2:317633/1‑145 (MQ=255)                 gTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAg                                                                                                                                        <  1:317633/145‑1 (MQ=255)                 gTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGa                                                                                                                                    >  5:209490/1‑149 (MQ=255)                 gTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGa                                                                                                                                    >  1:361114/1‑149 (MQ=255)                     aGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTc                                                                                                                                >  1:185450/1‑149 (MQ=255)                         cGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCTGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTcc‑‑cc                                                                                                                           <  3:121133/148‑1 (MQ=255)                                 gcgGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCGCCTGGTGATCGaatg                                                                                                                                       <  7:155570/130‑3 (MQ=255)                                 gcgGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCGCCTGGTGATCGaatg                                                                                                                                       >  8:155570/1‑128 (MQ=255)                                         gACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTc                                                                                                            >  7:201231/1‑147 (MQ=255)                                         gACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCaa                                                                                                          <  6:209490/149‑1 (MQ=255)                                         gACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCaa                                                                                                          <  2:361114/149‑1 (MQ=255)                                          aCAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCCCCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAag                                                                                                         >  5:299171/1‑149 (MQ=255)                                           cAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAagc                                                                                                        <  6:57223/149‑1 (MQ=255)                                              gggTGATGATGCCGTCGAGGATGTAGCGCTCACTCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGGCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAagcagc                                                                                                     <  7:79141/149‑1 (MQ=255)                                              gggTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTcc‑‑cc                                                                                                                           >  8:318408/1‑127 (MQ=255)                                              gggTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTcc‑‑cc                                                                                                                           <  7:318408/127‑1 (MQ=255)                                              gggTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTc                                                                                                            <  6:175524/142‑1 (MQ=255)                                              gggTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTc                                                                                                            >  5:175524/1‑142 (MQ=255)                                               ggTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAagcag                                                                                                      >  2:30966/1‑147 (MQ=255)                                               ggTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAagcag                                                                                                      >  6:82303/1‑147 (MQ=255)                                               ggTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAgcagca                                                                                                    <  7:291947/149‑1 (MQ=255)                                                 tgatgaTGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAgg                                                                                                  <  2:185450/149‑1 (MQ=255)                                                   atgatgCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTa                                                                                                >  6:3264/1‑149 (MQ=255)                                                         ccGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAgcgc                                                                                          >  3:203252/1‑149 (MQ=255)                                                              gAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGa                                                                                      <  1:30966/148‑1 (MQ=255)                                                              gAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAg                                                                                     >  2:300235/1‑149 (MQ=255)                                                                 gATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGt                                                                                    >  2:315322/1‑147 (MQ=255)                                                                 gATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCa                                                                                  <  1:255633/149‑1 (MQ=255)                                                                          cTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGTCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCAt                                                                         <  4:203252/149‑1 (MQ=255)                                                                             aCGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAg                                                                                 >  1:166899/1‑138 (MQ=255)                                                                             aCGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAg                                                                                 <  2:166899/138‑1 (MQ=255)                                                                               gCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGAtg                                                                    >  6:123810/1‑149 (MQ=255)                                                                                  gCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGAtgctg                                                                 <  1:315322/149‑1 (MQ=255)                                                                                   ctctTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGAtgctg                                                                 >  3:45722/1‑148 (MQ=255)                                                                                   ctctTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGACAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGAtgctc                                                                 >  5:228210/1‑147 (MQ=255)                                                                                        cgtcgATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTgcagca                                                            <  5:82303/148‑1 (MQ=255)                                                                                                  gaaCGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAagcagc                                                                                                     >  3:35534/1‑97 (MQ=255)                                                                                                  gaaCGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAagcagc                                                                                                     <  4:35534/97‑1 (MQ=255)                                                                                                  gaaCGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGt                                                 >  7:172587/1‑149 (MQ=255)                                                                                                           cgcgACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAg                                         <  7:133899/148‑1 (MQ=255)                                                                                                             cgACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTc                                      <  8:172587/149‑1 (MQ=255)                                                                                                              gACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCa                                     >  5:166598/1‑149 (MQ=255)                                                                                                               aCCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCAt                                    <  5:123810/149‑1 (MQ=255)                                                                                                                  ggCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGAc                                 <  8:201231/149‑1 (MQ=255)                                                                                                                         ggTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCAcc                          >  5:135495/1‑149 (MQ=255)                                                                                                                           tCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACTGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCgg                        <  6:299171/149‑1 (MQ=255)                                                                                                                              gCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGAc                                 >  5:234136/1‑137 (MQ=255)                                                                                                                              gCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGAc                                 <  6:234136/137‑1 (MQ=255)                                                                                                                                 aGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCTAACCATATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGt                    <  1:300235/149‑1 (MQ=255)                                                                                                                                          ccttCGATCGCCTGGTGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCgg                        >  5:342986/3‑134 (MQ=255)                                                                                                                                          ccttCGATCGCCTGGTGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCgg                        <  6:342986/132‑1 (MQ=255)                                                                                                                                          ccttCGATCGCCTGGTGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCcagcag         >  7:296512/3‑149 (MQ=255)                                                                                                                                            ttCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGACATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCCAGCAGtt       >  2:221055/1‑149 (MQ=255)                                                                                                                                               gATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCCAGCAGTTgg     <  1:221055/148‑1 (MQ=255)                                                                                                                                                aTCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATa                                                          >  8:7598/1‑94 (MQ=255)                                                                                                                                                aTCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATa                                                          <  7:7598/94‑1 (MQ=255)                                                                                                                                                  cACCAGGCGATCGAAGGGGATTTCC‑‑CCGGCATCCAGGCGTTCAATCAGGAGGTTCAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCCAGCAGTTGGCTg  <  6:228210/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     CGATGGTCTGGGTCAGGTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCCAGCAGTTGGCTG  >  NC_002947/5263820‑5264112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑