Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,263,972 C→T 8.2% R92H (CGC→CAC)  zinU ← P‑loop guanosine triphosphatase‑dependent zinc chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,263,9720CT 8.2% 172.7 / 4.6 61R92H (CGC→CAC) zinUP‑loop guanosine triphosphatase‑dependent zinc chaperone
Reads supporting (aligned to +/- strand):  ref base C (28/28);  new base T (3/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

GTCAGGTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCCAGCAGTTGGCTGTCGATGC  >  NC_002947/5263831‑5264119
                                                                                                                                             |                                                                                                                                                     
gTCAGGTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGaa                                                                                                                                                <  1:172330/149‑1 (MQ=255)
     gTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAg                                                                                                                                               <  1:317633/145‑1 (MQ=255)
     gTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAg                                                                                                                                               >  2:317633/1‑145 (MQ=255)
     gTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGa                                                                                                                                           >  5:209490/1‑149 (MQ=255)
     gTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGa                                                                                                                                           >  1:361114/1‑149 (MQ=255)
         aGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTc                                                                                                                                       >  1:185450/1‑149 (MQ=255)
             cGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCTGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTcc‑‑cc                                                                                                                                  <  3:121133/148‑1 (MQ=255)
                     gcgGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCGCCTGGTGATCGaatg                                                                                                                                              >  8:155570/1‑128 (MQ=255)
                     gcgGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCGCCTGGTGATCGaatg                                                                                                                                              <  7:155570/130‑3 (MQ=255)
                             gACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTc                                                                                                                   >  7:201231/1‑147 (MQ=255)
                             gACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCaa                                                                                                                 <  6:209490/149‑1 (MQ=255)
                             gACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCaa                                                                                                                 <  2:361114/149‑1 (MQ=255)
                              aCAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCCCCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAag                                                                                                                >  5:299171/1‑149 (MQ=255)
                               cAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAagc                                                                                                               <  6:57223/149‑1 (MQ=255)
                                  gggTGATGATGCCGTCGAGGATGTAGCGCTCACTCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGGCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAagcagc                                                                                                            <  7:79141/149‑1 (MQ=255)
                                  gggTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTcc‑‑cc                                                                                                                                  >  8:318408/1‑127 (MQ=255)
                                  gggTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTcc‑‑cc                                                                                                                                  <  7:318408/127‑1 (MQ=255)
                                  gggTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTc                                                                                                                   <  6:175524/142‑1 (MQ=255)
                                  gggTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTc                                                                                                                   >  5:175524/1‑142 (MQ=255)
                                   ggTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAagcag                                                                                                             >  2:30966/1‑147 (MQ=255)
                                   ggTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAagcag                                                                                                             >  6:82303/1‑147 (MQ=255)
                                   ggTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAgcagca                                                                                                           <  7:291947/149‑1 (MQ=255)
                                     tgatgaTGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAgg                                                                                                         <  2:185450/149‑1 (MQ=255)
                                       atgatgCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTa                                                                                                       >  6:3264/1‑149 (MQ=255)
                                             ccGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAgcgc                                                                                                 >  3:203252/1‑149 (MQ=255)
                                                  gAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGa                                                                                             <  1:30966/148‑1 (MQ=255)
                                                  gAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAg                                                                                            >  2:300235/1‑149 (MQ=255)
                                                     gATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGt                                                                                           >  2:315322/1‑147 (MQ=255)
                                                     gATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCa                                                                                         <  1:255633/149‑1 (MQ=255)
                                                              cTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGTCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCAt                                                                                <  4:203252/149‑1 (MQ=255)
                                                                 aCGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAg                                                                                        >  1:166899/1‑138 (MQ=255)
                                                                 aCGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAg                                                                                        <  2:166899/138‑1 (MQ=255)
                                                                   gCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGAtg                                                                           >  6:123810/1‑149 (MQ=255)
                                                                      gCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGAtgctg                                                                        <  1:315322/149‑1 (MQ=255)
                                                                       ctctTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGAtgctg                                                                        >  3:45722/1‑148 (MQ=255)
                                                                       ctctTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGACAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGAtgctc                                                                        >  5:228210/1‑147 (MQ=255)
                                                                            cgtcgATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTgcagca                                                                   <  5:82303/148‑1 (MQ=255)
                                                                                      gaaCGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAagcagc                                                                                                            >  3:35534/1‑97 (MQ=255)
                                                                                      gaaCGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAagcagc                                                                                                            <  4:35534/97‑1 (MQ=255)
                                                                                      gaaCGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGt                                                        >  7:172587/1‑149 (MQ=255)
                                                                                               cgcgACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAg                                                <  7:133899/148‑1 (MQ=255)
                                                                                                 cgACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTc                                             <  8:172587/149‑1 (MQ=255)
                                                                                                  gACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCa                                            >  5:166598/1‑149 (MQ=255)
                                                                                                   aCCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCAt                                           <  5:123810/149‑1 (MQ=255)
                                                                                                      ggCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGAc                                        <  8:201231/149‑1 (MQ=255)
                                                                                                             ggTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCAcc                                 >  5:135495/1‑149 (MQ=255)
                                                                                                               tCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACTGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCgg                               <  6:299171/149‑1 (MQ=255)
                                                                                                                  gCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGAc                                        >  5:234136/1‑137 (MQ=255)
                                                                                                                  gCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGAc                                        <  6:234136/137‑1 (MQ=255)
                                                                                                                     aGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCTAACCATATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGt                           <  1:300235/149‑1 (MQ=255)
                                                                                                                              ccttCGATCGCCTGGTGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCgg                               <  6:342986/132‑1 (MQ=255)
                                                                                                                              ccttCGATCGCCTGGTGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCgg                               >  5:342986/3‑134 (MQ=255)
                                                                                                                              ccttCGATCGCCTGGTGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCcagcag                >  7:296512/3‑149 (MQ=255)
                                                                                                                                ttCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGACATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCCAGCAGtt              >  2:221055/1‑149 (MQ=255)
                                                                                                                                   gATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCCAGCAGTTgg            <  1:221055/148‑1 (MQ=255)
                                                                                                                                    aTCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATa                                                                 <  7:7598/94‑1 (MQ=255)
                                                                                                                                    aTCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATa                                                                 >  8:7598/1‑94 (MQ=255)
                                                                                                                                      cACCAGGCGATCGAAGGGGATTTCC‑‑CCGGCATCCAGGCGTTCAATCAGGAGGTTCAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCCAGCAGTTGGCTg         <  6:228210/148‑1 (MQ=255)
                                                                                                                                         cAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCCAGCAGTTGGCTGTCGa     >  3:237507/1‑149 (MQ=255)
                                                                                                                                            gCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCCAGCAGTTGGCTGTCGATGc  >  5:305844/1‑149 (MQ=255)
                                                                                                                                            gCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCCAGCAGTTGGCTGTCGATGc  >  1:117756/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                     
GTCAGGTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCC‑‑CCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCCAGCAGTTGGCTGTCGATGC  >  NC_002947/5263831‑5264119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑