| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,305,662 | C→T | 10.3% | L530L (CTG→CTA) ‡ | recC ← | multifunctional ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease/ssDNA endonuclease recombinase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,305,662 | 0 | C | T | 10.3% | 154.0 / 5.8 | 58 | L530L (CTG→CTA) ‡ | recC | multifunctional ATP‑dependent DNA helicase/dsDNA/ssDNA exonuclease/ssDNA endonuclease recombinase subunit |
| Reads supporting (aligned to +/- strand): ref base C (23/29); new base T (3/3); total (26/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.92e-01 | |||||||||||
GGGCCGATCAGTGCCGCGTCCAGGCCACCGATTTCATCGTAGGGTTCA‑ATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCG > NC_002947/5305516‑5305800 | gggCCGATCAGTGCCGCGTCCAGGCCACCGATTTCATCGTAGGGTTCA‑ATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAg < 1:224799/149‑1 (MQ=255) ggCCGATCAGTGCCGCGTCCAGGCCACCGATTTCATCGTAGGGTTCA‑ATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAgg < 5:6395/149‑1 (MQ=255) tGCCGCGTCCAGGCCACCGATTTCATCGTAGGGTTCAGATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCGAGCCTGGGCTTGCCCGc > 1:64065/1‑149 (MQ=255) ccGCGTCCAGGCCACCGATTTCATCGTAGGGTTCA‑ATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCGAGCCTGGGCTTGCCCGccg < 2:64065/148‑3 (MQ=255) ccGCGTCCAGGCCACCGATTTCATCGTAGGGTTCA‑ATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTgc > 8:135523/1‑149 (MQ=255) gcgGCCAGGCCACCGATTTCATCGTAGGGTTCA‑ATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTgcgc < 3:242581/149‑1 (MQ=255) gCCACCGATTTCATCGTAGGGTTCA‑ATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGTAAGCCCAGGCTCGCCCGCTGCGCGGCGTCaa < 1:346701/149‑1 (MQ=255) cGATTTCATCGTAGGGTTCA‑ATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGccc < 6:135191/149‑1 (MQ=255) cGATTTCATCGTAGGGTTCA‑ATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGccc < 2:293930/149‑1 (MQ=255) aTCGTAGGGTTCA‑ATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCTCAGGCTCGCCCGCTGTGCGGCGTCAAGGCCCCAACGGa > 3:332318/1‑149 (MQ=255) tAGGGTTCA‑ATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGc > 6:30915/1‑148 (MQ=255) gggTTCA‑ATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCa < 7:135523/148‑1 (MQ=255) ggTTCA‑ATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCCACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCACCGGATGCCAgc > 5:336918/1‑149 (MQ=255) gTTCA‑ATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCCGCGGGCAAGCCCAGCCTCGCCCGCTGCGCCGCGTCAAGGCCCCAACGGATGACAgcg > 3:185622/1‑149 (MQ=255) ttCA‑ATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAgcgc > 1:26875/1‑149 (MQ=255) cA‑ATGCCATCACAGGCCTCACGCACCCCCACTGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGcc < 8:144293/148‑1 (MQ=255) a‑aTGCCATCACAGGCCTCA‑CCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTc < 5:209153/149‑1 (MQ=255) tGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTc > 6:48902/1‑148 (MQ=255) tCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCa > 6:236435/1‑149 (MQ=255) cacaGGCCTCACCCACCCCCACGCCATAACCCAACAGCAAGCCGCGCACGCCGAAACGCCAACTGTTCTCATCGAGCCCCCCCGGCAAGCCCAGCCTCGCCCCCTGCGCGGCGTCAAGGCCCCAACGGATGCCACCGCCCTCGATCCAg > 3:120733/1‑149 (MQ=255) caGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCg < 5:127060/149‑1 (MQ=255) cacccaccCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGc > 2:78016/1‑149 (MQ=255) acccacccCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCg > 3:183990/1‑149 (MQ=255) ccacccCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTg < 2:97587/149‑1 (MQ=255) acccCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTggg > 1:317585/1‑149 (MQ=255) ccccACCGCATAACCCAACACCCAGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCa < 4:309914/149‑1 (MQ=255) cccACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAg < 5:30915/149‑1 (MQ=255) ccACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGAGCAGCGTggtcgcg > 8:159275/1‑144 (MQ=255) aTAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGtttt > 1:161865/1‑149 (MQ=255) aCCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTcg < 2:317585/148‑1 (MQ=255) cccATCAGCATGCGGCGCAGGCCGATACGCCAACTGTTGTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCTGCGCCCTCGATCCAGGGGTGCAGCGTGGGCAGGTCGTTTTcgcg < 4:185622/149‑1 (MQ=255) cccAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTcgcg > 6:144224/1‑149 (MQ=255) cccAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCAGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTcgcg < 7:67478/149‑1 (MQ=255) ccAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTcgcg > 4:318063/1‑148 (MQ=255) ccAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTcgcg > 3:253806/1‑148 (MQ=255) aCAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATg > 5:192143/1‑149 (MQ=255) cAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGc < 6:224706/149‑1 (MQ=255) aGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCGCTACGGATGCCAGCGCCCTCGATCCAGCGGTGGAGCGTGGGCAGGTCGTTTTCGCGGATGcc < 4:120733/149‑1 (MQ=255) aTGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGc < 6:302250/145‑1 (MQ=255) aTGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGc > 5:302250/1‑145 (MQ=255) ggcgCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCgg < 5:48902/149‑1 (MQ=255) ggcgCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCgg > 8:135622/1‑149 (MQ=255) ggCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCgg < 3:318063/149‑1 (MQ=255) gCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCTCAGGCTCGCCCGCTGTGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGa < 4:332318/149‑1 (MQ=255) gCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTc < 8:171960/93‑1 (MQ=255) gCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTc > 7:171960/1‑93 (MQ=255) aaaCGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGc < 1:78016/149‑1 (MQ=255) aaaCGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGc < 2:26875/149‑1 (MQ=255) ccAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGAGCGCGGACTGCCGGCa > 1:35487/1‑149 (MQ=255) ctgttctgttCGAGCCCGGCGTGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGTACgcc < 4:183990/149‑3 (MQ=255) ttctgttcGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCcag < 7:135622/149‑1 (MQ=255) aGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAg < 5:236435/149‑1 (MQ=255) aGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAg < 5:145318/149‑1 (MQ=255) aGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCACCGCCCTCGAACCAGCGGTGCAGCCTGGGCAGCTCGTTTTCGAGGATGACGAAGCGGGCGCGGACTGCCGCCACGTCCAGCAGGTCGAg > 8:41825/1‑149 (MQ=255) gcggGCGAGCCTGGGCTTGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTc > 4:226526/1‑149 (MQ=255) cggGCGAGCCTGGGCTTGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGttt > 3:16731/1‑92 (MQ=255) cggGCGAGCCTGGGCTTGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGttt < 4:16731/92‑1 (MQ=255) cggGCGAGCCTGGGCTTGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCg < 3:226526/149‑1 (MQ=255) | GGGCCGATCAGTGCCGCGTCCAGGCCACCGATTTCATCGTAGGGTTCA‑ATGCCATCACAGGCCTCACCCACCCCCACGGCATAACCCAACAGCATGCGGCGCAGGCCGAAACGCCAACTGTTCTGTTCGAGCCCGGCGGGCAAGCCCAGGCTCGCCCGCTGCGCGGCGTCAAGGCCCCAACGGATGCCAGCGCCCTCGATCCAGCGGTGCAGCGTGGGCAGGTCGTTTTCGCGGATGCCGAAGCGGGCGCGGACTGCCGGCACGTCCAGCAGGTCGAGCACTTCG > NC_002947/5305516‑5305800 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |