Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,356,811	G→A	7.7%	L650L (CTC→CTT)	infB ←	translation initiation factor IF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,356,811	0	G	A	7.7%	188.3 / 4.0	65	L650L (CTC→CTT)	infB	translation initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base G (27/33);  new base A (2/3);  total (29/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

TACTGCATTCGAAGCCAGCGCCAGGTTGGCATCGCTCTCGGTGATACCACCGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGCCAGCTTGACCTCGCGATACTTGCCTTGACGGAACAGGGCAACTTCACGGGCTTTCTT  >  NC_002947/5356664‑5356948                                                                                                                                                      |                                                                                                                                          tACTGCATTCGAAGCCAGCGCCAGGTTGGCATCGGTGTCGGTGATACCACCGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCGAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCTGTCTTga                                                                                                                                          <  6:165827/149‑1 (MQ=255)   cTGCATTCGAAGCCAGCGCCAGGTTGGCATCGCTCTCGGTGATACCACCGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCATGCGCTTCCAGAGAACCACGCACATCGGTCTTgag                                                                                                                                         <  1:49360/149‑1 (MQ=255)    tGCATTCGAAGCCAGCGCCAGGTTGGCATCGCTCTCGGTGATACCACCGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGaa                                                                                                                                       >  5:227006/1‑149 (MQ=255)      cATTCGAAGCCAGCGCCAGGTTGGCATCGCTCTCGGTGATACCACCGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAAc                                                                                                                                      >  3:337576/1‑148 (MQ=255)        ttCGAAGCCAGCGCCAGGTTGGCATCGCTCTCGGTGATACCACCGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGAt                                                                                                                                   <  3:339543/149‑1 (MQ=255)           gAAGCCAGCGCCAGGTTGGCATCGCTCTCGGTGATACCACCGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGTTCTCAAGACCGATGt                                                                                                                                 <  4:270295/148‑1 (MQ=255)           gAAGCCAGCGCCAGGTTGGCATCGCTCTCGGTGATACCACCGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGTTCTCAAGACCGATGt                                                                                                                                 >  3:270295/1‑148 (MQ=255)              gCCAGCGCCAGGTTGGCATCGCTCTCGGTGATACCACCGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAg                                                                                                                             >  8:196701/1‑149 (MQ=255)              gCCAGCGCCAGGTTGGCATCGCTCTCGGTGATACCACCGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAg                                                                                                                             >  3:31704/1‑149 (MQ=255)              gCCAGCGCCAGGTTGGCATCGCTCTCGGTGATACCACCGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAg                                                                                                                             <  4:31704/149‑1 (MQ=255)              gCCAGCGCCAGGTTGGCATAGCTCTCGGTGATACCACCGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAg                                                                                                                             <  5:122187/149‑1 (MQ=255)               ccAGCGCCAGGTTGGCATCGCTCTCGGTGATACCACCGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAgg                                                                                                                            <  5:329814/149‑1 (MQ=255)                cAGCGCCAGGTTGGCATCGCTCTCGGTGATACCACCGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAggg                                                                                                                           <  7:196701/149‑1 (MQ=255)                 aGCGCCAGGTTGGCATCGCTCTCGGTGATACCACCGACGCCCCCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGCGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGCGt                                                                                                                          >  7:338468/1‑149 (MQ=255)                      cAGGTTGGCATCGCTCTCGGTGATACCACCGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTttct                                                                                                                     >  3:272745/1‑149 (MQ=255)                            cgCAGCGCACTCGGGGCTTCCCCCGACGCCACCACCCATCACGCGCACTTGCTCTTCGTGGTGTGCCC‑GGGCGCGGAGTGAACCCTGCA‑GAGCTTGCATATATACACGCACATATGTATTGATAACGATGTTGAGGGTTTtcttctctt                                                                                                               <  8:338468/148‑1 (MQ=255)                              cATCGCTCTCGGTGATACCACCGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTcc                                                                                                             >  7:34054/1‑149 (MQ=255)                                tCGCTCTCGGTGATACCACCGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCt                                                                                                            >  5:348304/1‑148 (MQ=255)                                   ctctCGGTGATACCACCGACGCCACCCCCAATCACGCGCACTTGCACTTCGTCGT‑TTCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGAcc                                                                                                        <  6:306970/149‑1 (MQ=255)                                        ggTGATACCACCGACGCCACCACCACTCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATgg                                                                                                   <  2:107969/149‑1 (MQ=255)                                        ggTGATACCACCGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATg                                                                                                    <  6:213839/148‑1 (MQ=255)                                         gTGATACCTCCGACGCCACCCCGAATCACGCGCTCTTGCTCTCCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGACCCACGCACATCGGGCTTGAGCACGATGTTGAGGGTTTTCTTCTCTACCTGACCGATGGt                                                                                                  <  8:303320/149‑1 (MQ=255)                                               ccaccGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGaa                                                                                            <  4:272745/149‑1 (MQ=255)                                                 accGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGTTCTCAAGACCGAt                                                                                                                                   <  8:43684/108‑1 (MQ=255)                                                 accGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGa                                                                                          <  4:27496/149‑1 (MQ=255)                                                 accGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGa                                                                                          <  2:53488/149‑1 (MQ=255)                                                  ccGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGAt                                                                                         >  5:311812/1‑149 (MQ=255)                                                      cGCCAACACCACTCACGCGCACTTGCACTTCCTCGT‑TGCCCAGGCCGCCTAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACCTCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTATTCCTGACCCATGGTCTCGAAGATGttt                                                                                     <  8:172554/149‑1 (MQ=255)                                                        ccaccaccaATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTc                                                                                   <  7:283979/149‑1 (MQ=255)                                                        ccaccaccaATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTc                                                                                   <  7:315858/149‑1 (MQ=255)                                                          accaccaATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTc                                                                                   >  4:152249/1‑147 (MQ=255)                                                                    aCGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGc                                                                       >  5:272893/1‑149 (MQ=255)                                                                      gcgcACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGt                                                                     <  6:227006/149‑1 (MQ=255)                                                                      gcgcACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGt                                                                     >  2:309152/1‑149 (MQ=255)                                                                      gcgcACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGt                                                                     >  8:312761/1‑149 (MQ=255)                                                                        gcacttgcacttCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGa                                                                   <  6:311812/149‑1 (MQ=255)                                                                          acttgcacttCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAg                                                                  >  5:254324/1‑148 (MQ=255)                                                                                acttCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGc                                                           >  4:177900/1‑149 (MQ=255)                                                                                  ttCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCGCATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGc                                                           >  4:99071/1‑147 (MQ=255)                                                                                  ttCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGCCa                                                         >  5:142297/1‑149 (MQ=255)                                                                                    cgtcgt‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGCCAg                                                        <  6:142297/148‑1 (MQ=255)                                                                                       cgt‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATg                                                                                                    <  7:144347/101‑1 (MQ=255)                                                                                       cgt‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATg                                                                                                    >  8:144347/1‑101 (MQ=255)                                                                                       cgt‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGCCAGCtt                                                     >  3:326320/1‑148 (MQ=255)                                                                                        gt‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGCCAGCTTGa                                                   >  7:58536/1‑149 (MQ=255)                                                                                              ccAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACgg                                                             <  8:326908/134‑1 (MQ=255)                                                                                              ccAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACgg                                                             >  7:326908/1‑134 (MQ=255)                                                                                               cAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGCCAGCTTGACCTcgc                                             >  1:178469/1‑149 (MQ=255)                                                                                               cAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCATCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGCCAGCTTGACCTcgc                                             <  6:348304/149‑1 (MQ=255)                                                                                               cAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCATCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGCCAGCTTGACCTcgc                                             <  8:58536/149‑1 (MQ=255)                                                                                                        gAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGCCAGCTTGACCTCGCGATACTTGc                                    <  7:312761/149‑1 (MQ=255)                                                                                                        gAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGCCAGCTTGACCTCGCGATACTTGc                                    <  2:178469/149‑1 (MQ=255)                                                                                                              aCCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGCCAGCTTGACCTCGCGATACTTGCCTTGa                               <  6:254324/148‑1 (MQ=255)                                                                                                                 cTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGCCAGCTTGACCTCGCGATACTTGCCTTGACgg                            >  1:316213/1‑148 (MQ=255)                                                                                                                  tGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGCCAGCTTGACCTCGCGATACTTGc                                    <  5:346980/139‑1 (MQ=255)                                                                                                                  tGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGCCAGCTTGACCTCGCGATACTTGc                                    >  6:346980/1‑139 (MQ=255)                                                                                                                        cgcTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGCCAGCt                                                      <  7:45652/116‑1 (MQ=255)                                                                                                                        cgcTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGCCAGCt                                                      >  8:45652/1‑116 (MQ=255)                                                                                                                           ttCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTCGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACgg                                                             <  7:260352/106‑1 (MQ=255)                                                                                                                           ttCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTAGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACgg                                                             >  8:260352/1‑106 (MQ=255)                                                                                                                             ccAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGCCAGCTTGACCTCGCGATACTTGCCTTGACGGAACAGGGCaa                  <  2:316213/147‑1 (MQ=255)                                                                                                                                       ctcaacaTCGTTCTCAAGACCGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGcc                                                          <  2:27434/93‑1 (MQ=38)                                                                                                                                       ctcaacaTCGTTCTCAAGACCGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGcc                                                          >  1:27434/5‑97 (MQ=38)                                                                                                                                        cGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGCCAGCTTGACCTCGCGATACTTGCCTTGACGGAACAGGGCAACTTCACGGGCttt     <  4:326320/149‑1 (MQ=255)                                                                                                                                         gCACATCGGGCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGCCAGCTTGACCTCGCGATACTTGCCTTGACGGAACAGGGCAACTTCACGGGCTttc    <  3:152249/149‑1 (MQ=255)                                                                                                                                           agaTCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGCCAGCTTGACCTCGCGATACTTGCCTTGACGGAACAGGGCAACTTCACGGGCTttctt  <  1:309152/147‑1 (MQ=255)                                                                                                                                           acaTCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTATCGAAGAGGTTATACAGCTTGACGGCGTGAGCACGGGCAAGCTTCACCTCTCCATACTTTCCTTGACGGAACAGGGCAACTTCAAGGTATttct   >  7:221675/1‑148 (MQ=255)                                                                                                                                                      |                                                                                                                                          TACTGCATTCGAAGCCAGCGCCAGGTTGGCATCGCTCTCGGTGATACCACCGACGCCACCACCAATCACGCGCACTTGCACTTCGTCGT‑TGCCCAGGCCGCCGAGCGAACCCTGCA‑GCGCTTCCAGAGAACCACGCACATCGGTCTTGAGAACGATGTTGAGGGTTTTCTTCTCTTCCTGACCCATGGTCTCGAAGATGTTTTCCAGCTTGCCGGCGTGAGCACGGGCCAGCTTGACCTCGCGATACTTGCCTTGACGGAACAGGGCAACTTCACGGGCTTTCTT  >  NC_002947/5356664‑5356948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑