Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,361,071 A→T 12.0% intergenic (‑78/+23) PP_t49 ← / ← PP_t48 tRNA‑Met/tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,361,0710AT12.0% 118.0 / 5.8 48intergenic (‑78/+23)PP_t49/PP_t48tRNA‑Met/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base A (23/19);  new base T (3/3);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCTT  >  NC_002947/5360930‑5361214
                                                                                                                                              |                                                                                                                                               
tGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCggg                                                                                                                                          >  8:237016/1‑149 (MQ=255)
       gAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAGAGCCCGCTTTAGCGGGCtttct                                                                                                                                    <  6:143292/148‑2 (MQ=255)
       gAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTTTAGCGGGCtttct                                                                                                                                    <  6:193136/148‑2 (MQ=255)
                  cTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACACCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTgg                                                                                                                        >  8:302661/1‑149 (MQ=255)
                            tGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAg                                                                                                              <  2:94527/149‑1 (MQ=255)
                             gAGCCCGACGAGCTACCAAGCTGCACCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATACAAACATTCACCTACAAGAAAGCCCGATAAAGCGGGCACTCAAACTTTAATTGGAACCCAAAAgg                                                                                                             >  7:51338/1‑149 (MQ=255)
                              aGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAgg                                                                                                             <  7:237016/148‑1 (MQ=255)
                                cccGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGa                                                                                                          >  8:252583/1‑149 (MQ=255)
                                  cgacgaGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAgg                                                                                                             >  7:242646/1‑144 (MQ=255)
                                  cgacgaGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAgg                                                                                                             <  8:242646/144‑1 (MQ=255)
                                       aGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAAc                                                                                                   >  2:281152/1‑149 (MQ=255)
                                         cTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCACTGTTGAGGGTCAAATCCAAACATTCACATACAAGAAAGCCAGATAAAGCGGGCTCTCAAGCTTTATTTGGTACAGAGAAGGGGACTCGAAcg                                                                                                  >  1:279510/1‑148 (MQ=255)
                                                      ccACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTAt                                                                                      <  2:215174/147‑1 (MQ=255)
                                                      ccACCCCGCGACAAAGCTGGGGCAAAAGTAAAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTAt                                                                                      >  1:215174/1‑147 (MQ=255)
                                                          cccGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGca                                                                                 >  1:98142/1‑148 (MQ=255)
                                                            cgcgACAAAGCTGGGGCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACaa                                                                              >  3:56744/1‑149 (MQ=255)
                                                            cgcgACAAAGCTGGGGCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACaa                                                                              >  1:66420/1‑149 (MQ=255)
                                                            cgcgACAAAGCTGGGGCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACaa                                                                              >  1:290023/1‑149 (MQ=255)
                                                                aCAAAGGTGGGGCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAaccac                                                                          <  7:302661/149‑1 (MQ=255)
                                                                aCAAAGCTGGGGCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAaccac                                                                          <  7:252583/149‑1 (MQ=255)
                                                                         gggCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGtt                                                                 >  2:14773/1‑149 (MQ=255)
                                                                          ggCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTg                                                                <  2:98142/149‑1 (MQ=255)
                                                                           gCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGc                                                               >  3:157385/1‑149 (MQ=255)
                                                                                 gTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCgt                                                             <  3:122711/145‑1 (MQ=255)
                                                                                 gTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCgt                                                             >  4:122711/1‑145 (MQ=255)
                                                                                    taAGACCGACCC‑GTGTTGAGTGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTAc                                                       <  4:56744/148‑1 (MQ=255)
                                                                                         ccGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCgtgt                                                           >  7:50309/1‑139 (MQ=255)
                                                                                         ccGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCgtgt                                                           <  8:50309/139‑1 (MQ=255)
                                                                                         ccGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTc                                                 <  2:290023/149‑1 (MQ=255)
                                                                                            aaCC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTccac                                              >  6:248766/1‑149 (MQ=255)
                                                                                                      gAGTGTCAAATCCAAACATTGACCTACAAGAAAGACCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGAATCGAACCCCTACACCCTATGGGCACAACCACCTCATGGTTGCGTGTCTACCAATTCCACCACCTCgg                                      <  6:161733/148‑1 (MQ=255)
                                                                                                               aTCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAAtactact                            <  5:197456/149‑1 (MQ=255)
                                                                                                                 ccAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCAACCACCTCGGCAAtactactac                          >  2:185567/1‑149 (MQ=255)
                                                                                                                  cAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATactactact                         <  1:281152/149‑1 (MQ=255)
                                                                                                                         tCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGAGTCTACCAATTCCACCCCCTCGGCAATACTACAACTTGAAAcc                  >  5:109452/1‑149 (MQ=255)
                                                                                                                            ccTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAACGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGt               <  3:107449/149‑1 (MQ=255)
                                                                                                                              tACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTAGCAAGTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTa             >  5:228435/1‑149 (MQ=255)
                                                                                                                               aCAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTAc            >  7:341804/1‑149 (MQ=255)
                                                                                                                                cAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTa             <  4:157385/147‑1 (MQ=255)
                                                                                                                                cAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACt           >  2:11562/1‑149 (MQ=255)
                                                                                                                                   gagaGCCCGCTTTAGCGGGCTTTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCt        >  2:316353/4‑149 (MQ=255)
                                                                                                                                   gagaGCCCGCTTTAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCt        >  7:314824/4‑149 (MQ=255)
                                                                                                                                    aaaGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTg       <  5:248766/149‑1 (MQ=255)
                                                                                                                                     gaGCCCGCTTTAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGc      >  4:162103/2‑149 (MQ=255)
                                                                                                                                     aaGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATAATACTACTTGAAACCCGTTACTTCTGc      >  7:116138/1‑149 (MQ=255)
                                                                                                                                       gCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGctc    <  2:66420/149‑1 (MQ=255)
                                                                                                                                         ccGCTTTAGCGGGCTTTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCtt  <  1:316353/149‑1 (MQ=255)
                                                                                                                                         ccGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCtt  <  1:11562/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                               
TGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACC‑CTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCTT  >  NC_002947/5360930‑5361214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑