Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,384,527	G→C	6.7%	G331R (GGC→CGC) ‡	lldP →	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,384,527	0	G	C	6.7%	187.9 / 3.8	60	G331R (GGC→CGC) ‡	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand):  ref base G (30/26);  new base C (2/2);  total (32/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

AGCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGCACCGCGA  >  NC_002947/5384388‑5384671                                                                                                                                            |                                                                                                                                                  aGCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGa                                                                                                                                          >  3:178269/1‑149 (MQ=255)  gCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGCCCGGCGCGa                                                                                                                                          >  6:71804/1‑148 (MQ=255)   ccGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGCCCGGCGCGaaa                                                                                                                                        <  5:71804/149‑3 (MQ=255)      ttGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTAc                                                                                                                                     <  5:225621/149‑1 (MQ=255)              ccAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑gt                                                                                                                            <  6:180496/149‑1 (MQ=255)                aaGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑gtgt                                                                                                                          >  8:111675/1‑149 (MQ=255)                       agccagccTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACtt                                                                                                                   <  6:72733/149‑1 (MQ=255)                          cagccTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGc                                                                                                                <  6:340937/149‑1 (MQ=255)                            gccTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCa                                                                                                              >  1:211845/1‑149 (MQ=255)                                  cccGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGc                                                                                                        <  5:206611/149‑1 (MQ=255)                                    cGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTc                                                                                                                               <  8:73754/125‑1 (MQ=255)                                    cGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTc                                                                                                                               >  7:73754/1‑125 (MQ=255)                                          gagctGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGAc                                                                                                <  7:191215/145‑1 (MQ=255)                                            tttGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGAc                                                                                                >  8:69019/1‑147 (MQ=255)                                                gCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGAc                                                                                                >  8:191215/1‑143 (MQ=255)                                                gCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTg                                                                                          >  4:167884/1‑149 (MQ=255)                                                    gaTCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGa                                                                                      <  2:211845/149‑1 (MQ=255)                                                    gaTCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGa                                                                                      <  3:167884/149‑1 (MQ=255)                                                          cAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGacc                                                                                    <  7:69019/145‑3 (MQ=255)                                                               ccTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCa                                                                           >  1:100237/1‑149 (MQ=255)                                                                 tGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCAcc                                                                         >  8:115131/1‑149 (MQ=255)                                                                   gTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGa                                                                       >  3:311922/1‑149 (MQ=255)                                                                        cGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGtt                                                                  <  7:189133/149‑1 (MQ=255)                                                                            ccTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCcg                                                              >  2:63871/1‑149 (MQ=255)                                                                             cTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCcgc                                                             >  2:25132/1‑149 (MQ=255)                                                                              tGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATc                                                                     <  6:215816/140‑1 (MQ=255)                                                                              tGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATc                                                                     >  5:215816/1‑140 (MQ=255)                                                                                aTCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGcc                                                          >  6:309585/1‑149 (MQ=255)                                                                                   cTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGAc                                                       >  5:129773/1‑149 (MQ=255)                                                                                            cTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGc                                               <  2:100237/148‑1 (MQ=255)                                                                                             tGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCcg                                             >  6:185634/1‑149 (MQ=255)                                                                                                  aCCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGgt                                        >  4:113371/1‑149 (MQ=255)                                                                                                  aCCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGgt                                        >  1:310779/1‑149 (MQ=255)                                                                                                    cATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGgtgt                                      <  5:185634/149‑1 (MQ=255)                                                                                                    cATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGgtgt                                      >  5:310715/1‑149 (MQ=255)                                                                                                      tCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGtt                                     >  4:7301/1‑148 (MQ=255)                                                                                                         ggACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAg                                 >  8:259138/1‑149 (MQ=255)                                                                                                          gACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCaa                                  <  1:25132/147‑1 (MQ=255)                                                                                                          gACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCaa                                  <  5:309585/147‑1 (MQ=255)                                                                                                          gACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCa                                   <  3:7301/146‑1 (MQ=255)                                                                                                          gACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGc                                <  7:259138/149‑1 (MQ=255)                                                                                                          gACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGAGCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGc                                <  6:183370/149‑1 (MQ=255)                                                                                                              cTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGa                            <  4:311922/149‑1 (MQ=255)                                                                                                               tGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTc                                    >  8:73297/1‑140 (MQ=255)                                                                                                               tGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTc                                    <  7:73297/140‑1 (MQ=255)                                                                                                                 aaGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGAccc                         <  7:115131/149‑1 (MQ=255)                                                                                                                       ttCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGAt                                                                                     >  4:332424/1‑83 (MQ=255)                                                                                                                          aaGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGAcc                          >  7:203734/1‑139 (MQ=255)                                                                                                                          aaGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGAcc                          <  8:203734/139‑1 (MQ=255)                                                                                                                            ggcggcTTTCGCGCCGGGCGGCGCGATGTACAACTTCAGTTTTCAACTTCGCCAGCCCGCCCCGCGCCCAGGTGGTGATCAAGACGGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTGCAAGCTCGACCCGATCTCCGcc               >  1:245927/1‑149 (MQ=255)                                                                                                                               gtacatCGCGCCGCCCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTcc                  >  4:341586/6‑142 (MQ=255)                                                                                                                               gtacatCGCGCCGCCCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTcc                  <  3:341586/137‑1 (MQ=255)                                                                                                                               ggcTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCg           <  6:310715/149‑1 (MQ=255)                                                                                                                                gcTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAGGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCgg          >  3:1158/1‑149 (MQ=255)                                                                                                                                 cTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGc                                <  7:229922/126‑1 (MQ=255)                                                                                                                                 cTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGc                                >  8:229922/1‑126 (MQ=255)                                                                                                                                    tCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGCAcc      >  5:133223/1‑149 (MQ=255)                                                                                                                                      gcgcCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGCACcgc    >  3:213803/1‑149 (MQ=255)                                                                                                                                      gcgcCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGCACcg     <  3:113371/148‑1 (MQ=255)                                                                                                                                        gcCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACATCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGCACCGCGa  >  3:135620/1‑149 (MQ=255)                                                                                                                                            |                                                                                                                                                  AGCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTC‑GTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGCACCGCGA  >  NC_002947/5384388‑5384671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑