Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	445,105	A→T	19.4%	intergenic (+78/‑210)	PP_0367 → / → PP_0368	hypothetical protein/acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	445,105	0	A	T	19.4%	56.5 / 10.5	31	intergenic (+78/‑210)	PP_0367/PP_0368	hypothetical protein/acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (15/10);  new base T (3/3);  total (18/13)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.68e-01

CGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAACT  >  NC_002947/444967‑445253                                                                                                                                           |                                                                                                                                                      cgcAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGACCAGACAAAATTGTTGTCTga                                                                                                                                             <  2:156885/149‑2 (MQ=255)       gggcgtcAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGc                                                                                                                                       <  7:247930/146‑1 (MQ=255)       gctcggcAAGGCATCCGACGAAATGCTTCGCCGCATCATCGACACCTACGCCTGAATACTTGCGGCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGc                                                                                                                                       <  8:298974/147‑1 (MQ=255)                   aTCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGc                                                                                                                           <  6:333242/149‑1 (MQ=255)                       gacgaAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAAAATTGTTGTCTGATc                                                                                                                                           >  4:37213/1‑129 (MQ=255)                       gacgaAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAAAATTGTTGTCTGATc                                                                                                                                           <  3:37213/129‑1 (MQ=255)                                          tcatcGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTTCCCCCGCATTCGTACGCTAAAACAACAATTTTTTAAGGTCGGGCCTCTTAGCGGGGC‑AGCCCGCGCCAAACAGGGGTtg                                                                                                   >  7:56890/1‑149 (MQ=255)                                                                            cccTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTTCCCGCGCATGCGTCCGCTCAGACAACAATTTTTTGTGGTGGGGCCTAATAGCCGGCA‑AGCCCGCGCCGACAAGGGGTTGTGGGATGGCATGAGTGATTTTGCATTTTTAGTgg                                                                 >  6:155680/1‑149 (MQ=255)                                                                            cccTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTc                                                                                                                                 <  1:70690/86‑1 (MQ=255)                                                                            cccTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTc                                                                                                                                 >  2:70690/1‑86 (MQ=255)                                                                               accttCAGCGCCTGCCTTGTCGGCGCGGGCTTGCCCGCGAATGCGTCCTACCACAAAACAATTTTGTCTGGCCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGt                                                              <  7:334441/146‑1 (MQ=38)                                                                                                     gAGCGGGCTTGCCCGCTAATGCGTCCGATCAGAAAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGt                                               <  1:228687/142‑1 (MQ=255)                                                                                                           gCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCGATCCTGGa                                     >  7:221795/1‑146 (MQ=255)                                                                                                                cccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGc                             >  7:150484/1‑149 (MQ=255)                                                                                                                 gcgcgAATGCGTACGATCAGACAACAATTTTGTATGGTCGGGCCTCTTCGCGTGCA‑AGCGCGCTCCTTCAAGGGTTTGTTGCTTGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGTCTCCTTAGTCCATCCTTGa                                     <  4:1761/139‑1 (MQ=255)                                                                                                                 ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAGAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGc                             >  1:98631/1‑149 (MQ=255)                                                                                                                 ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGTCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGACCATCATTGACAGATAgg                             >  3:1761/1‑147 (MQ=255)                                                                                                                  cgcgAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt                            >  5:28086/1‑148 (MQ=255)                                                                                                                          cGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAAc                   >  8:38742/1‑149 (MQ=255)                                                                                                                           gcccGACCAGACAAAATTGTTGTCTGATCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGAttt                                                                               <  5:252000/86‑1 (MQ=18)                                                                                                                           gcacGACCAGACAAAATTGTTGTCTGATCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGAttt                                                                               >  6:252000/4‑88 (MQ=18)                                                                                                                           gTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACg                  >  8:71125/1‑149 (MQ=255)                                                                                                                             ccGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAg                         >  2:46324/1‑140 (MQ=255)                                                                                                                             ccGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAg                         <  1:46324/140‑1 (MQ=255)                                                                                                                             ccGACCAGACAAAATTGTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGTCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGt                 >  6:106845/1‑148 (MQ=38)                                                                                                                               gATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAg              >  4:275588/1‑149 (MQ=255)                                                                                                                               gATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATCGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAg              >  6:364900/1‑149 (MQ=255)                                                                                                                                     acaacaATTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCAt                                                  >  2:295711/1‑107 (MQ=255)                                                                                                                                     acaacaATTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCa         >  1:257373/1‑148 (MQ=255)                                                                                                                                     acaacaATTTTCTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCAt                                                  <  1:295711/107‑1 (MQ=255)                                                                                                                                     acaacaATTTTCCCGTGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGCCATGGCATGACTGATTTCCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCaa        <  8:221795/149‑1 (MQ=255)                                                                                                                                       aacaaTTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGa                          >  7:97998/1‑129 (MQ=255)                                                                                                                                       aacaaTTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGa                          <  8:97998/129‑1 (MQ=255)                                                                                                                                       aacaaTTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTaa               <  6:354344/140‑1 (MQ=255)                                                                                                                                       aacaaTTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTaa               >  5:354344/1‑140 (MQ=255)                                                                                                                                       aacaaTTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCaaa       >  3:2099/1‑148 (MQ=255)                                                                                                                                          aaTTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAaacaac   >  6:129744/1‑149 (MQ=255)                                                                                                                                          aaTTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAaacaac   <  5:155680/149‑1 (MQ=255)                                                                                                                                           aTTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGCTGAGTGGCTTGGCATGACTGATTTCCCATTTTTCGTGGCGTAAATGGCACCAAAGTCCATCCTTGACAGAAACGTGAGCTAAAAGTAAGTTTCAAACAACt  >  4:314428/1‑149 (MQ=255)                                                                                                                                           |                                                                                                                                                      CGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCA‑AGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAACT  >  NC_002947/444967‑445253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑