Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,407,046 T→G 6.7% N124T (AAC→ACC)  PP_4751 ← amino acid ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,407,0460TG 6.7% 179.4 / 4.8 60N124T (AAC→ACC) PP_4751amino acid ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (30/26);  new base G (2/2);  total (32/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.23e-01

GCGATACGTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCGGCGG  >  NC_002947/5406899‑5407194
                                                                                                                                                   |                                                                                                                                                    
gcgatACGTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGtt                                                                                                                                                     <  2:97200/149‑1 (MQ=255)
    tACGTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTgcgc                                                                                                                                                 <  4:73293/149‑1 (MQ=255)
       gTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCgcag                                                                                                                                               >  8:187417/1‑148 (MQ=255)
             gctgGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGGCGGCTTCGTTGGCGCTGCGGCCGAAGGTGCGGATTTGCGCCAGGCTGACGTTGCgctgccgcgt                                                                                                                                         >  8:255367/1‑143 (MQ=255)
               tgGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCGAACGTGGGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAg                                                                                                                                      >  8:309938/1‑149 (MQ=255)
                ggCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAgg                                                                                                                                     <  7:255367/149‑1 (MQ=255)
                   caccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGc                                                                                                                                  >  1:360100/1‑149 (MQ=255)
                   caccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGGTGCTGCGCAACGTCAgcctg                                                                                                                                  <  6:211538/149‑5 (MQ=255)
                      caGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCggg                                                                                                                               <  6:127/149‑1 (MQ=255)
                       agagCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCggg                                                                                                                               >  1:85962/1‑148 (MQ=255)
                        gagCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGaa                                                                                                                             >  1:61610/1‑149 (MQ=255)
                        gagCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGaa                                                                                                                             >  8:163926/1‑149 (MQ=255)
                             gcgcgcCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGt                                                                                                                        >  3:231372/1‑149 (MQ=255)
                             gcgcgcCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGt                                                                                                                        <  8:68855/149‑1 (MQ=255)
                              cgcgcCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGtt                                                                                                                       <  8:216559/149‑1 (MQ=255)
                              cgcgcCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGtt                                                                                                                       <  7:151272/149‑1 (MQ=255)
                                        aaTGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTc                                                                                                                                        >  3:240873/1‑122 (MQ=255)
                                        aaTGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTc                                                                                                                                        <  4:240873/122‑1 (MQ=255)
                                        aaTGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTg                                                                                                              >  5:277816/1‑148 (MQ=255)
                                               ggCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACAcc                                                                                                       <  6:277816/148‑1 (MQ=255)
                                                gCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACAGTCAGGTGCGGGAACAGGTTGAACGACTGGAacacaa                                                                                                      >  1:271828/1‑146 (MQ=255)
                                                 ccTTGTCAGCCAGACCAAAGCGTTCGAGCAGTTGAAAGCAGCGGGAGTCGGCTTCATTCGCGCTGCGGCCCAAGGTGCGGATTTTCGCCAGGCTGACGTATCGCAGCACCGACAGGTGCGGCAACAGGTTGACCCACTGGAACCCCAtc                                                                                                    >  8:68370/1‑148 (MQ=255)
                                                     gTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGaa                                                                                                                             >  2:58704/1‑120 (MQ=255)
                                                     gTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGaa                                                                                                                             <  1:58704/120‑1 (MQ=255)
                                                        agccagccCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGGCGGCTTCTTTGGCCCTGCGGCCCAAGCTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCACTgc                                                                                             <  4:352410/149‑1 (MQ=255)
                                                         gccagccCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATgcg                                                                                            >  5:142375/1‑149 (MQ=255)
                                                          ccagccCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACt                                                                                                               >  7:91987/1‑129 (MQ=255)
                                                          ccagccCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACt                                                                                                               <  8:91987/129‑1 (MQ=255)
                                                            agccCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCATCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCg                                                                                         >  6:75146/1‑149 (MQ=255)
                                                             gccCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGACCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGGACAGGTTGAAAGACTGGAACACCAAACCAATGCGCCgg                                                                                        >  6:23157/1‑149 (MQ=255)
                                                              cccAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGgc                                                                                       >  1:55731/1‑149 (MQ=255)
                                                               ccAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGAGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGgcg                                                                                      >  5:118518/1‑149 (MQ=255)
                                                                cAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATgcgc                                                                                           <  4:332384/143‑1 (MQ=255)
                                                                cAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATgcgc                                                                                           >  3:332384/1‑143 (MQ=255)
                                                                cAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGgcgc                                                                                     <  7:309938/149‑1 (MQ=255)
                                                                 aaCGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGgcgg                                                                                     >  4:237903/1‑147 (MQ=255)
                                                                      gTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACtt                                                                                >  4:325175/1‑148 (MQ=255)
                                                                      gTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCATCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTc                                                                               <  5:75146/149‑1 (MQ=255)
                                                                       ttCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTca                                                                              <  2:61610/149‑1 (MQ=255)
                                                                       ttCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTAGAACACCATACCAATGCGCCGGCGCAACTTca                                                                              <  7:187417/149‑1 (MQ=255)
                                                                          gAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTcag                                                                             >  2:290434/1‑147 (MQ=255)
                                                                            gCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGt                                                                         >  2:231692/1‑149 (MQ=255)
                                                                                         gCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCgca                                                                                                                                                >  6:154933/1‑65 (MQ=255)
                                                                                         gCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCgca                                                                                                                                                <  5:154933/65‑1 (MQ=255)
                                                                                                   gCTTGTTTGGCGCTGCGGCCCATGGTGGGGATTTGCGCCAGGGTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACAGCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGcc                                                  <  6:118518/149‑1 (MQ=255)
                                                                                                   gCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGcc                                                  <  6:142375/149‑1 (MQ=255)
                                                                                                     ttcttTGGCGCAGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAg                                                >  7:216673/1‑149 (MQ=255)
                                                                                                            gcgcTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGAt                                         <  2:85962/149‑1 (MQ=255)
                                                                                                                     cccAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGcc                                <  7:68370/149‑1 (MQ=255)
                                                                                                                     cccAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGc                                 >  1:257329/1‑148 (MQ=255)
                                                                                                                                  tttGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGAc                    <  8:216673/148‑1 (MQ=255)
                                                                                                                                       gcCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGcaatcc                            >  7:220522/1‑135 (MQ=255)
                                                                                                                                       gcCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGccc              >  8:124287/1‑149 (MQ=255)
                                                                                                                                       gcCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGccc              <  1:290434/149‑1 (MQ=255)
                                                                                                                                         cAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCt            <  3:325175/149‑1 (MQ=255)
                                                                                                                                           ggCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTg          <  2:257329/149‑1 (MQ=255)
                                                                                                                                            ccTGACGGTGCTGCGCAACGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAg                                                >  3:170105/2‑110 (MQ=39)
                                                                                                                                            ccTGACGGTGCTGCGCAACGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAg                                                <  4:170105/109‑1 (MQ=39)
                                                                                                                                             cTGACGGTGCTGCGCAACGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGAt                                         >  2:1232/1‑116 (MQ=255)
                                                                                                                                                aCGTTGCGCTGGACCGTCAGGTGCGGGACCAGGTGGAACGACTGTAACACCATACCAATCCGCCGGCGCAACTTCAGCAGCTCTGTACGGGGCAACGCCTTGGCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTcgg     <  2:271828/149‑1 (MQ=255)
                                                                                                                                                  gTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGCCTGGAACACCATACCAATACGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTcggcg   >  1:279829/1‑149 (MQ=255)
                                                                                                                                                   ttGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTcggcgg  <  1:231692/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCGATACGTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCGGCGG  >  NC_002947/5406899‑5407194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑