Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,520,364 C→T 6.9% A137V (GCC→GTC)  PP_4853 → DMT superfamily permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,520,3640CT 6.9% 169.2 / 2.0 58A137V (GCC→GTC) PP_4853DMT superfamily permease
Reads supporting (aligned to +/- strand):  ref base C (24/30);  new base T (2/2);  total (26/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

ACCAGTGCCACCAACATGGGCATCATCCTCTCGCTGATGCCGCTGATGTCACTGGCGCTGTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGCCTATGCGCTGTACAGCTTCCTGCTGAAGAAATGGCAGCTGCGCCTGCCGCCAATGCAGCTGCTGTACCTGCAG  >  NC_002947/5520216‑5520509
                                                                                                                                                    |                                                                                                                                                   
aCCAGTGCCACCAACATGGGCATCATCCTCTCGCTGATGCCGCTGATGTCACTGGCGCTGTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGc                                                                                                                                                     >  4:146168/1‑149 (MQ=255)
         accaACATGGGCATCATCCTCTCGCTGATGCCGCTGATGTCACTGGCGCTGTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCAccc                                                                                                                                            >  2:315566/1‑149 (MQ=255)
         accaACATGGGCATCATCCTCTCGCTGATGCCGCTGATGTCACTGGCGCTGTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGAGCTTGAAGTGGTCAGTGCCGGCCa                                                                                                                                               >  3:173122/1‑146 (MQ=255)
         accaACATGGGCATCATCCTCTCGCTGATGCCGCTGATGTCACTGGCGCTGTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGAGCTTGAAGTGGTCAGTGCCGGCCa                                                                                                                                               <  4:173122/146‑1 (MQ=255)
              cATGGGCATCATCCTCTCGCTGATGCCGCTGATGTCACTGGCGCTGTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGGGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCa                                                                                                                                       <  3:188983/149‑1 (MQ=255)
               aTGGGCATCATCCTCTCGCTGATGCCGCTGATGTCACTGGCGCTGTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAg                                                                                                                                      >  5:34733/1‑149 (MQ=255)
                        atcCTCTCGCTGATGCCGCTGATGTCACTGGCGCTGTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTgca                                                                                                                           <  6:34733/149‑1 (MQ=255)
                                gCTGATGCCGCTGATGTCACTGGCGCTGTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGc                                                                                                                   >  8:288688/1‑149 (MQ=255)
                                    aTGCCGCTGATGTCACTGGCGCTGTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGcc                                                                                                                                        >  4:277801/1‑126 (MQ=255)
                                    aTGCCGCTGATGTCACTGGCGCTGTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGcc                                                                                                                                        <  3:277801/126‑1 (MQ=255)
                                    aTGCCGCTGATGTCACTGGCGCTGTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCCCTTGCTgcagcaggctggc                                                                                                                 <  1:315566/149‑8 (MQ=255)
                                    aTGCCGCTGATGTCACTGGCGCTGTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGaa                                                                                                               <  3:146168/149‑1 (MQ=255)
                                        cGCTGATGTCACTGGCGCTGTCCATCGCCTGGCTTGTCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAgc                                                                                                           <  4:191987/149‑1 (MQ=255)
                                         gCTGATGTCACTGGCGCTGTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAgcg                                                                                                          <  8:53304/149‑1 (MQ=255)
                                         gCTGAGGTCACTGGCGCCGCCCATCGCCTGGCCTGGCCAGCGCCGGAGCTACGGCGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAgcg                                                                                                          <  8:55002/149‑1 (MQ=255)
                                             aTGTCACTGGCGCTGTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAgcggc                                                                                                        <  2:338332/147‑1 (MQ=255)
                                             aTGTCACTGGCGCTGTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGa                                                                                                      >  5:327157/1‑149 (MQ=255)
                                                 cACTGGCGCTGTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAggg                                                                                                                    >  1:273858/1‑131 (MQ=255)
                                                 cACTGGCGCTGTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAggg                                                                                                                    <  2:273858/131‑1 (MQ=255)
                                                  aCTGGCGCTGTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGTCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGa                                                                                                                <  6:172364/134‑1 (MQ=255)
                                                  aCTGGCGCTGTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGTCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGa                                                                                                                >  5:172364/1‑134 (MQ=255)
                                                    tGGCGCTGTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGtt                                                                                                >  6:271026/1‑148 (MQ=255)
                                                           gTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCtgg                                                                                        >  6:284952/1‑149 (MQ=255)
                                                            tCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCtgg                                                                                        <  2:321383/148‑1 (MQ=255)
                                                              cATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCtggtg                                                                                      >  3:80939/1‑148 (MQ=255)
                                                               aTCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCtggtgg                                                                                     >  7:284992/1‑148 (MQ=255)
                                                               aTCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCtggtgg                                                                                     >  7:305998/1‑148 (MQ=255)
                                                                        cgtGGCCACCGCCTGAGGCACGGTGCCCTGCGGGGCGCGGTGGTGTCAATCTTCGGCCGGCCTGCAGTGGTCAGTGCCGGGCCCCCCGCCAGCC‑‑TGCTGCAGCAAGGGCGGAACAGCGGCGACCTGTTGATGCGGGTGGCAACCTTGGc                                                                           <  4:347001/147‑1 (MQ=255)
                                                                         ttGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGcc                                                                          >  1:15555/1‑149 (MQ=255)
                                                                                  gcCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGCCTATGCGCTg                                                                 <  5:271026/149‑1 (MQ=255)
                                                                                   ccTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAgg                                                                                                                     <  2:30877/96‑1 (MQ=255)
                                                                                   ccTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAgg                                                                                                                     >  1:30877/1‑96 (MQ=255)
                                                                                       aGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGTCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGc                                                                           <  5:148143/134‑1 (MQ=255)
                                                                                       aGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGTCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGc                                                                           >  6:148143/1‑134 (MQ=255)
                                                                                       aGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGTCGACCTGTTGATGCTGGTGGCAACCTTGGCCTATGCGCTGTACa                                                             <  2:40797/148‑1 (MQ=255)
                                                                                       aGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGCCTATGCGCTGTAc                                                              <  7:87210/147‑1 (MQ=255)
                                                                                          tACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCc‑‑tg                                                                                                                                <  7:36706/78‑1 (MQ=255)
                                                                                          tACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCc‑‑tg                                                                                                                                >  8:36706/1‑78 (MQ=255)
                                                                                            cGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGCCTATGCGCTGTACAGCTTc                                                        <  5:284952/148‑1 (MQ=255)
                                                                                            cGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTCAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGCCTATGCGCTGTACAGCTTcc                                                       >  5:286909/1‑149 (MQ=255)
                                                                                                  ccTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGCCTATGCGCTGTACAGCTTCctgctg                                                  >  7:315194/1‑148 (MQ=255)
                                                                                                   ctgctgGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGCCTATGCGCTGTACAGCTTCCTGCTgaa                                                <  2:15555/149‑1 (MQ=255)
                                                                                                         ggCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGt                                                                                                 >  6:193678/1‑94 (MQ=255)
                                                                                                         ggCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGt                                                                                                 <  5:193678/94‑1 (MQ=255)
                                                                                                         ggCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGCCTATGCGCTGTACAGCTTCCTGGTGAAGAAAt                                           <  6:286909/148‑1 (MQ=255)
                                                                                                         ggCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGCCTATGCGCTGTACAGCTTCCTGCTGAAGAAATg                                          <  7:288688/149‑1 (MQ=255)
                                                                                                          gcgcGGTGGTGTCATTCTTCGGCGGGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGCCTATGCGCTGTACAGCTTCCTGCTGAAGAAATgg                                         <  6:371795/149‑1 (MQ=255)
                                                                                                           cgcgGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGCCTATGCGCTGTACAGCTTCCTGCTGAAGAAATGGc                                        <  1:46082/149‑1 (MQ=255)
                                                                                                                   tgtCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGCCTATGCGCTGTACAGCTTCCTGCTGAAGAAATGGCAGCTgcgc                                <  8:305998/149‑1 (MQ=255)
                                                                                                                    gtCATTCTTCGGCGTGATTGAAGTGGTCAGTGCCGGCAACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGc                                                                           >  5:219028/1‑105 (MQ=255)
                                                                                                                        ttcttcGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGCCCTGGTGATGCTGGTGGCCACCTTGGCCTATGCGCTGTACAGCTTCCTGCTGAAGAAATGGCCGCTGCGCCTgcc                           >  2:94284/1‑149 (MQ=255)
                                                                                                                           ttcGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGCCTATGCGCTGTACAGCTTCCTGCTGAAGAAATGGCAGCTGCGCCTgccgc                         >  2:42451/1‑148 (MQ=255)
                                                                                                                           ttcGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGCCTATGCGCTGTACAGCTTCCTGCTGAAGAAATGGCAGCTGCGCCTgccgc                         >  2:316710/1‑148 (MQ=255)
                                                                                                                              ggCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGCCTATGCGCTGTACAGCTTCCTGCTGAAGAAATGGCAGCTGCGCCTGCCGCCaa                      <  1:42451/148‑1 (MQ=255)
                                                                                                                                cGTGCTTGAAGTGGTCAGTGCAGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGCCTATGCGCTGTACAGCTTCctgct                                                   <  6:252856/117‑1 (MQ=255)
                                                                                                                                cGTGCTTGAAGTGGTCAGTGCAGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGCCTATGCGCTGTACAGCTTCctgct                                                   >  5:252856/1‑117 (MQ=255)
                                                                                                                                      tGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGCCTATGCGCTGTACAGCTTCCTGCTGAAGAAATGGCAGCTGCGCCTGCCGCCAATGCAgctgc             >  1:350837/1‑149 (MQ=255)
                                                                                                                                         aGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGCCTATGCGCTGTACAGCTTCCTGCTGAAGAAATGGCAGCTGCGCCTGCCGCCAATGCAGCTGCTGt          <  6:166060/149‑1 (MQ=255)
                                                                                                                                                 gTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGCCTATGCGCTGTACAGCTTCCTGCTGAAGAAATGGCAGCTGCGCCTGCCGCCAATGCAGCTGCTGTACCTGCAg  <  6:327157/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACCAGTGCCACCAACATGGGCATCATCCTCTCGCTGATGCCGCTGATGTCACTGGCGCTGTCCATCGCCTGGCTTGGCCAGCGCCTGAGCTACGGTGCCCTGCTGGGCGCGGTGGTGTCATTCTTCGGCGTGCTTGAAGTGGTCAGTGCCGGCCACCCCGCCAGCC‑‑TGCTGCAGCAAGGGCTGAACAGCGGCGACCTGTTGATGCTGGTGGCAACCTTGGCCTATGCGCTGTACAGCTTCCTGCTGAAGAAATGGCAGCTGCGCCTGCCGCCAATGCAGCTGCTGTACCTGCAG  >  NC_002947/5520216‑5520509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑