Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,529,190	C→A	7.3%	intergenic (‑404/+190)	ydfG ← / ← livF‑II	bifunctional NADP‑dependent 3‑hydroxy acid dehydrogenase/malonic semialdehyde reductase/ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,529,190	0	C	A	7.3%	199.1 / 4.1	63	intergenic (‑404/+190)	ydfG/livF‑II	bifunctional NADP‑dependent 3‑hydroxy acid dehydrogenase/malonic semialdehyde reductase/ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (32/26);  new base A (3/2);  total (35/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCGCCTGCAGCGGTCTGTCCAAGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTC  >  NC_002947/5529047‑5529338                                                                                                                                                |                                                                                                                                                          tGCCGCATGCAGCGGTATGTCCAATGTTTTTACAGAGAGTTATCCACATGATTGTCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGc                                                                                                                                                      <  1:110639/149‑1 (MQ=255)                    tCCAAGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCtt                                                                                                                                     >  6:62697/1‑147 (MQ=255)                      cAAGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑Gc                                                                                                                            >  8:212311/1‑149 (MQ=255)                       aaGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑Gc                                                                                                                            >  6:85621/1‑148 (MQ=255)                        aGGTTTTTACACAGACTTATCCACAGGATTGGCACTGCACTTGCAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGa                                                                                                                          <  4:114986/149‑1 (MQ=255)                              ttAAACAGAGTTATCGAGAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTTGGATAATCATCCACAACGGTGATATGAACAAAAATTGATCAAATCGCTCAAGGCCTTGAGTATCAAGCCCTGT‑‑‑‑A‑GCGATTTga                                                                                                                     <  4:352134/148‑2 (MQ=21)                                acacAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTCAAGGCCTTGAGTATCAAGCCCTGT‑‑‑‑A‑GCGATTTgatc                                                                                                                   <  6:297480/148‑4 (MQ=37)                                        ttATCCACAGGATTGGCACTGCACATGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCAACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGtttt                                                                                                          >  8:55768/1‑149 (MQ=255)                                          aTCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTc                                                                                                         <  7:212311/148‑1 (MQ=255)                                          aTCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTc                                                                                                         >  5:9747/1‑148 (MQ=255)                                           tCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTcc                                                                                                        <  5:85621/148‑1 (MQ=255)                                                 ggATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGTAAAATCATCCACAACGGGTATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAggc                                                                                                  <  2:179520/148‑1 (MQ=255)                                                    ttGGAACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCt                                                                                              <  5:62697/149‑1 (MQ=255)                                                      ggCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACGATGTTTTCCACAGGCGGCTc                                                                                             <  3:36970/148‑1 (MQ=255)                                                         actgcactTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCcaca                                                                                         >  2:300515/1‑149 (MQ=255)                                                          ctgcactTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCacac                                                                                        >  5:32523/1‑149 (MQ=255)                                                          ctgcactTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCacac                                                                                        >  3:177420/1‑149 (MQ=255)                                                           tgcactTGAAGCACACGTAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCccac                                                                                        >  8:253724/1‑148 (MQ=255)                                                             cacaTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACt                                                                                       <  7:55768/147‑1 (MQ=255)                                                                         acGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATAGCTAAAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCAACATGTTTTCCACAGGCGGCTCCACACTATCCGTGGaa                                                                             >  1:266575/1‑145 (MQ=255)                                                                           gCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGt                                                                       >  6:118082/1‑149 (MQ=255)                                                                           gCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGt                                                                       >  4:74612/1‑149 (MQ=255)                                                                           gCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGt                                                                       >  1:184475/1‑149 (MQ=255)                                                                             agagATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTg                                                                     >  3:175025/1‑149 (MQ=255)                                                                              gagaTGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTgg                                                                    <  4:339090/149‑1 (MQ=255)                                                                                 aTGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCt                                                                 <  5:118082/149‑1 (MQ=255)                                                                                     tGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCtgtg                                                              >  3:73016/1‑148 (MQ=255)                                                                                      gAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGACGGCTCCACACTATCCGTGGAAAAGAGTTGGCCtgt                                                               >  4:151321/1‑146 (MQ=255)                                                                                      gAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGACGGCTCCACACTATCCGTGGAAAAGAGTTGGCCtgt                                                               <  3:151321/146‑1 (MQ=255)                                                                                               ggAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATgg                                                    <  4:73016/148‑1 (MQ=255)                                                                                                 aaaaTCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCtt                                                 >  8:247289/1‑149 (MQ=255)                                                                                                  aaaTCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTg                                                <  8:186800/149‑1 (MQ=255)                                                                                                       atcCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGaa                                            <  1:181611/148‑1 (MQ=255)                                                                                                       atcCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAg                                           <  8:34010/149‑1 (MQ=255)                                                                                                          cacaACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGaa                                        >  3:138546/1‑149 (MQ=255)                                                                                                           acaACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCtgtg                                                              >  8:102128/1‑126 (MQ=255)                                                                                                           acaACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCtgtg                                                              <  7:102128/126‑1 (MQ=255)                                                                                                           acaACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGaa                                        >  7:42017/1‑148 (MQ=255)                                                                                                              aCGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATg                                    <  8:42017/149‑1 (MQ=255)                                                                                                               cGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATgg                                   >  7:126076/1‑149 (MQ=255)                                                                                                                 gggATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGgt                                 <  4:177420/149‑1 (MQ=255)                                                                                                                 gggATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGgt                                 <  6:32523/149‑1 (MQ=255)                                                                                                                        gAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTc                          >  3:225470/1‑149 (MQ=255)                                                                                                                         aaCAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCg                         >  2:292815/1‑149 (MQ=255)                                                                                                                         aaCAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCg                         >  8:181986/1‑149 (MQ=255)                                                                                                                          aCAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATgg                                   <  1:49356/138‑1 (MQ=255)                                                                                                                          aCAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATgg                                   >  2:49356/1‑138 (MQ=255)                                                                                                                          aCAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCg                         <  4:138546/148‑1 (MQ=255)                                                                                                                          aCAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCg                         <  7:247289/148‑1 (MQ=255)                                                                                                                           cAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCgg                        >  2:325375/1‑148 (MQ=255)                                                                                                                           cAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCgg                        >  7:339083/1‑148 (MQ=255)                                                                                                                             aaaaaTTATCAAATCGCTTCATGTCTTGTTACTAAAGGCCTTGTCTAACCCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTc                          <  1:300515/149‑1 (MQ=255)                                                                                                                                  tGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTAt                                                                                     >  6:327272/1‑80 (MQ=255)                                                                                                                                  tGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTAt                                                                                     <  5:327272/80‑1 (MQ=255)                                                                                                                                  tGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAgg                 >  6:6000/1‑148 (MQ=255)                                                                                                                                   gATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAggg                >  2:42169/1‑148 (MQ=255)                                                                                                                                       ataTCGCTCAAGGCCTTGAGTATCAAGCCCTGT‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGTGTGTGTTTCGGGAGAAGGGAAc             >  6:250027/3‑147 (MQ=37)                                                                                                                                       ataTCGCTCAAGGCCTTGAGTATCAAGCCCTGT‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGa           >  3:2622/3‑149 (MQ=38)                                                                                                                                        aaTCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTgg                                                             >  1:248038/1‑98 (MQ=255)                                                                                                                                        aaTCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTgg                                                             <  2:248038/98‑1 (MQ=255)                                                                                                                                         aTCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAggg                <  4:171860/142‑1 (MQ=255)                                                                                                                                         aTCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAggg                >  3:171860/1‑142 (MQ=255)                                                                                                                                              tACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGttt    >  7:109121/1‑149 (MQ=255)                                                                                                                                                cAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGtttt   <  7:253724/148‑1 (MQ=255)                                                                                                                                                cAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTc  <  1:42169/149‑1 (MQ=255)                                                                                                                                                cAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTc  <  3:74612/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                          TGCCGCCTGCAGCGGTCTGTCCAAGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTC  >  NC_002947/5529047‑5529338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑