Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,529,212	T→G	5.3%	intergenic (‑426/+168)	ydfG ← / ← livF‑II	bifunctional NADP‑dependent 3‑hydroxy acid dehydrogenase/malonic semialdehyde reductase/ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,529,212	0	T	G	5.3%	238.8 / 4.3	76	intergenic (‑426/+168)	ydfG/livF‑II	bifunctional NADP‑dependent 3‑hydroxy acid dehydrogenase/malonic semialdehyde reductase/ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (38/34);  new base G (2/2);  total (40/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.20e-01

TCCAAGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGG  >  NC_002947/5529066‑5529353                                                                                                                                                   |                                                                                                                                                   tCCAAGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCtt                                                                                                                                                    >  6:62697/1‑147 (MQ=255)   cAAGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑Gc                                                                                                                                           >  8:212311/1‑149 (MQ=255)    aaGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑Gc                                                                                                                                           >  6:85621/1‑148 (MQ=255)     aGGTTTTTACACAGACTTATCCACAGGATTGGCACTGCACTTGCAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGa                                                                                                                                         <  4:114986/149‑1 (MQ=255)           ttAAACAGAGTTATCGAGAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTTGGATAATCATCCACAACGGTGATATGAACAAAAATTGATCAAATCGCTCAAGGCCTTGAGTATCAAGCCCTGT‑‑‑‑A‑GCGATTTga                                                                                                                                    <  4:352134/148‑2 (MQ=21)             acacAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTCAAGGCCTTGAGTATCAAGCCCTGT‑‑‑‑A‑GCGATTTgatc                                                                                                                                  <  6:297480/148‑4 (MQ=37)                     ttATCCACAGGATTGGCACTGCACATGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCAACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGtttt                                                                                                                         >  8:55768/1‑149 (MQ=255)                       aTCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTc                                                                                                                        <  7:212311/148‑1 (MQ=255)                       aTCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTc                                                                                                                        >  5:9747/1‑148 (MQ=255)                        tCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTcc                                                                                                                       <  5:85621/148‑1 (MQ=255)                              ggATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGTAAAATCATCCACAACGGGTATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAggc                                                                                                                 <  2:179520/148‑1 (MQ=255)                                 ttGGAACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCt                                                                                                             <  5:62697/149‑1 (MQ=255)                                   ggCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACGATGTTTTCCACAGGCGGCTc                                                                                                            <  3:36970/148‑1 (MQ=255)                                      actgcactTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCcaca                                                                                                        >  2:300515/1‑149 (MQ=255)                                       ctgcactTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCacac                                                                                                       >  3:177420/1‑149 (MQ=255)                                       ctgcactTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCacac                                                                                                       >  5:32523/1‑149 (MQ=255)                                        tgcactTGAAGCACACGTAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCccac                                                                                                       >  8:253724/1‑148 (MQ=255)                                          cacaTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACt                                                                                                      <  7:55768/147‑1 (MQ=255)                                                      acGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATAGCTAAAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCAACATGTTTTCCACAGGCGGCTCCACACTATCCGTGGaa                                                                                            >  1:266575/1‑145 (MQ=255)                                                        gCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGt                                                                                      >  6:118082/1‑149 (MQ=255)                                                        gCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGt                                                                                      >  4:74612/1‑149 (MQ=255)                                                        gCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGt                                                                                      >  1:184475/1‑149 (MQ=255)                                                          agagATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTg                                                                                    >  3:175025/1‑149 (MQ=255)                                                           gagaTGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTgg                                                                                   <  4:339090/149‑1 (MQ=255)                                                              aTGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCt                                                                                <  5:118082/149‑1 (MQ=255)                                                                  tGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCtgtg                                                                             >  3:73016/1‑148 (MQ=255)                                                                   gAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGACGGCTCCACACTATCCGTGGAAAAGAGTTGGCCtgt                                                                              <  3:151321/146‑1 (MQ=255)                                                                   gAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGACGGCTCCACACTATCCGTGGAAAAGAGTTGGCCtgt                                                                              >  4:151321/1‑146 (MQ=255)                                                                            ggAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATgg                                                                   <  4:73016/148‑1 (MQ=255)                                                                              aaaaTCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCtt                                                                >  8:247289/1‑149 (MQ=255)                                                                               aaaTCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTg                                                               <  8:186800/149‑1 (MQ=255)                                                                                    atcCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGaa                                                           <  1:181611/148‑1 (MQ=255)                                                                                    atcCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAg                                                          <  8:34010/149‑1 (MQ=255)                                                                                       cacaACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGaa                                                       >  3:138546/1‑149 (MQ=255)                                                                                        acaACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCtgtg                                                                             >  8:102128/1‑126 (MQ=255)                                                                                        acaACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCtgtg                                                                             <  7:102128/126‑1 (MQ=255)                                                                                        acaACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGaa                                                       >  7:42017/1‑148 (MQ=255)                                                                                           aCGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATg                                                   <  8:42017/149‑1 (MQ=255)                                                                                            cGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATgg                                                  >  7:126076/1‑149 (MQ=255)                                                                                              gggATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGgt                                                <  6:32523/149‑1 (MQ=255)                                                                                              gggATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGgt                                                <  4:177420/149‑1 (MQ=255)                                                                                                     gAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTc                                         >  3:225470/1‑149 (MQ=255)                                                                                                      aaCAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCg                                        >  2:292815/1‑149 (MQ=255)                                                                                                      aaCAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCg                                        >  8:181986/1‑149 (MQ=255)                                                                                                       aCAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATgg                                                  >  2:49356/1‑138 (MQ=255)                                                                                                       aCAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATgg                                                  <  1:49356/138‑1 (MQ=255)                                                                                                       aCAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCg                                        <  7:247289/148‑1 (MQ=255)                                                                                                       aCAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCg                                        <  4:138546/148‑1 (MQ=255)                                                                                                        cAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCgg                                       >  7:339083/1‑148 (MQ=255)                                                                                                        cAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCgg                                       >  2:325375/1‑148 (MQ=255)                                                                                                          aaaaaTTATCAAATCGCTTCATGTCTTGTTACTAAAGGCCTTGTCTAACCCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTc                                         <  1:300515/149‑1 (MQ=255)                                                                                                               tGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTAt                                                                                                    <  5:327272/80‑1 (MQ=255)                                                                                                               tGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTAt                                                                                                    >  6:327272/1‑80 (MQ=255)                                                                                                               tGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAgg                                >  6:6000/1‑148 (MQ=255)                                                                                                                gATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAggg                               >  2:42169/1‑148 (MQ=255)                                                                                                                    ataTCGCTCAAGGCCTTGAGTATCAAGCCCTGT‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGTGTGTGTTTCGGGAGAAGGGAAc                            >  6:250027/3‑147 (MQ=37)                                                                                                                    ataTCGCTCAAGGCCTTGAGTATCAAGCCCTGT‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGa                          >  3:2622/3‑149 (MQ=38)                                                                                                                     aaTCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTgg                                                                            <  2:248038/98‑1 (MQ=255)                                                                                                                     aaTCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTgg                                                                            >  1:248038/1‑98 (MQ=255)                                                                                                                      aTCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAggg                               <  4:171860/142‑1 (MQ=255)                                                                                                                      aTCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAggg                               >  3:171860/1‑142 (MQ=255)                                                                                                                           tACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGttt                   >  7:109121/1‑149 (MQ=255)                                                                                                                             cAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGtttt                  <  7:253724/148‑1 (MQ=255)                                                                                                                             cAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTc                 <  1:42169/149‑1 (MQ=255)                                                                                                                             cAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTc                 <  3:74612/149‑1 (MQ=255)                                                                                                                              aGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTcc                <  5:6000/149‑1 (MQ=255)                                                                                                                               gggCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCca               <  1:325375/149‑1 (MQ=255)                                                                                                                               gggCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCca               <  4:175025/149‑1 (MQ=255)                                                                                                                                 gCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCcaca             >  7:319627/1‑149 (MQ=255)                                                                                                                                    tGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACAtt           >  3:59583/1‑148 (MQ=255)                                                                                                                                     gATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCTCAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACAtt           <  1:183157/147‑1 (MQ=255)                                                                                                                                     gATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACAtt           >  4:132887/1‑147 (MQ=255)                                                                                                                                        aCTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTgag        >  6:78366/1‑147 (MQ=255)                                                                                                                                          tCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAgg                                <  2:248018/121‑1 (MQ=255)                                                                                                                                           cAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAggg   >  7:266487/1‑149 (MQ=255)                                                                                                                                            aaGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAgggg  <  8:109121/149‑1 (MQ=255)                                                                                                                                            aaGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAgggg  >  3:166022/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                   TCCAAGGTTTTTACACAGAGTTATCCACAGGATTGGCACTGCACTTGAAGCACACGCAGAGATGTTGAAAGCTTGGGAAAATCATCCACAACGGGGATATGAACAAAAAATGATCAAATCGCTACAGGGCTTGATACTCAAGGCCTTG‑‑‑‑A‑GCGATATGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGG  >  NC_002947/5529066‑5529353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑