Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,537,058 A→G 17.4% intergenic (+35/+68) nadE → / ← PP_4870 NH(3)‑dependent NAD(+) synthetase/azurin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,537,0580AG17.4% 124.9 / 19.4 58intergenic (+35/+68)nadE/PP_4870NH(3)‑dependent NAD(+) synthetase/azurin
Reads supporting (aligned to +/- strand):  ref base A (25/22);  new base G (4/6);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.36e-02

GCAGATCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCAC  >  NC_002947/5536912‑5537199
                                                                                                                                                  |                                                                                                                                             
gcAGATCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATAc                                                                                                                                              >  8:6891/1‑148 (MQ=255)
  aGATCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATAc                                                                                                                                              >  4:259569/1‑146 (MQ=255)
  aGATCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGa                                                                                                                                            <  3:259569/148‑1 (MQ=255)
     tcaaTGCCTTCCTGAATGGGCTGCGGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATATGTGGCCTGCAGGAGCGGGGTCACCCGCGAATACGAGGGt                                                                                                                                        <  4:180475/146‑1 (MQ=255)
            cTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGa                                                                                                                                      <  8:201902/144‑1 (MQ=255)
            cTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGa                                                                                                                                      >  7:201902/1‑144 (MQ=255)
             ttCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTACCCCGCGAAAAAGAGGGTGAACCCAc                                                                                                                                >  2:114726/1‑149 (MQ=255)
               ccTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCAcc                                                                                                                               >  7:312759/1‑148 (MQ=255)
                 tGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTGACCCGCGGAAAAGCCGGTGAACCCACCGAc                                                                                                                            >  3:62607/1‑149 (MQ=255)
                      ggacAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCGCCCGCGAATACGAGGGTGAACCCACCGACGCAt                                                                                                                        >  6:349888/4‑148 (MQ=255)
                       ggCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTc                                                                                                                      >  2:247546/1‑149 (MQ=255)
                          aGCCGGTTGACCAGGCTGCGTTCGACATCATCGTCGCCACGTCCCGCAAGACCCTGCACAAGCGCGAACTGCCGTTCGCTCATTCATAAGTGGCCTGCAGCAGCGGTTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTcgcg                                                                                                                   <  8:17087/149‑1 (MQ=255)
                           gccgcctGACCCGCCAGCGCTCGACATCACCCCCGCCCCCCACCGCACCACCCCCCACCCCCGCCACCTGCCGCTCGCTCCTTCATACGTGCCCTGCAGCAGCGGGTCCCCCCGCGAATACGAGGCTGAACCCACCGACGCATTCGCgg                                                                                                                  <  8:312759/143‑1 (MQ=255)
                           gCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                  <  8:40225/149‑1 (MQ=25)
                           gCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                  <  6:184564/149‑1 (MQ=25)
                           gCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCgg                                                                                                                  <  1:339428/149‑1 (MQ=255)
                           gCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAACTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCgg                                                                                                                  <  6:210111/149‑1 (MQ=255)
                             cGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGt                                                                                                                <  1:247546/149‑1 (MQ=255)
                             cGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGt                                                                                                                <  7:6891/149‑1 (MQ=255)
                                     cAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                  <  8:293970/139‑1 (MQ=21)
                                     cAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                  >  7:293970/1‑139 (MQ=21)
                                                                 ccTACCGCAATACCCAGCACAAGCGCTAACTGCCTTTCGCTCCTTGATATGTGGCCTGCTGGAGCGGGTTCCCCCGCTAATACGCGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGa                                                                            <  1:114726/149‑1 (MQ=38)
                                                                  cTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAACCCGCGAATACGAGGGTGTACCCACCGACGCATTAGCGGGTGAAACCGCTCCGGCAGGGGTCGCCGGCAGCCTTTGAt                                                                           >  1:100012/1‑149 (MQ=255)
                                                                    aCCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCa                                                                                    <  3:94060/138‑1 (MQ=255)
                                                                      cGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACt                                                                       >  3:240886/1‑149 (MQ=255)
                                                                         aaGACCCACCAAACGCGCTAACTGCGGTTGGCTCTTTTATAAGTGGCTTGCAGGAGGGGGTTCACCCGCTAATACGTGGGTTAACCCACGGACGCTTTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGa                                                                    <  6:120213/149‑1 (MQ=255)
                                                                          aGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                  <  1:192921/102‑1 (MQ=14)
                                                                          aGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                  >  2:187165/1‑102 (MQ=14)
                                                                          aGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                  >  2:192921/1‑102 (MQ=14)
                                                                          aGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGCCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                  <  1:187165/102‑1 (MQ=17)
                                                                              ccAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                  <  5:155747/98‑1 (MQ=14)
                                                                              ccAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                  >  6:155747/1‑98 (MQ=14)
                                                                               cAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAACCCCCCAATACACGGGTGCACCCACCGACGCATTCGCGGGTGAACCCCCTACTGGAGGGTTGCCACTCAGCCGATGAATACTAGaagcaga                                                              >  2:291752/1‑143 (MQ=255)
                                                                                aGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGTGGGTg                                                                                                               >  8:18906/1‑99 (MQ=255)
                                                                                aGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGTGGGTg                                                                                                               <  7:18906/99‑1 (MQ=255)
                                                                                   acaAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCtt                                                          <  5:349888/149‑1 (MQ=255)
                                                                                   acaAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAAACCGCGAAAAAGAGGGTGAAACCACCCACCCAATCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTGAGCCGTTGATTACTTTACGATGAACtt                                                          >  4:212607/1‑149 (MQ=255)
                                                                                     aaGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGGGGGTGAACCCCCCGCCGCATTAGCGGGTGAACCCGCGCCTGGAGGGTTCGCCGGCCGCCTTTGATTACTTGAGGATGACCTTgg                                                        >  5:127438/1‑148 (MQ=255)
                                                                                        cgcgAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGc                                                                                                        >  7:251529/1‑98 (MQ=255)
                                                                                        cgcgAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGc                                                                                                        <  8:251529/98‑1 (MQ=255)
                                                                                             aCTGCCGTTCCCTCCTTGATAAGGGGCCTCCCGGAGCGGGTTCCCCCGCGAATACGAGGGGGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTtcatc                                                <  6:127438/149‑1 (MQ=38)
                                                                                                 ccGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTTAACCAACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCg                                            <  7:316159/149‑1 (MQ=255)
                                                                                                       gCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCTAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGAt                                                                           <  7:159944/112‑1 (MQ=255)
                                                                                                                tAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATAAGAGGGTGAACCCACCGCCGCATTCGCGGGTGAACCCGCGCCGGGAGGGGTGGGCGGCAGCCGTTTATTACTTGACGAAGGCCGTGGCATTCATCATGGAGATTGGGCCCggg                              >  4:264725/1‑148 (MQ=19)
                                                                                                                   gTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAgg                          <  4:240886/149‑1 (MQ=255)
                                                                                                                     ggCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTg                        <  7:216306/149‑1 (MQ=255)
                                                                                                                       catGCAGTAGCGGGTTCACCCGCTAATACGAGGGTGAACCCACCGACGCATTCGCGGGTTAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCa                      <  3:212607/147‑1 (MQ=255)
                                                                                                                            aGGAGCGGGTTAACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCagaag                  >  2:111081/1‑148 (MQ=255)
                                                                                                                              gAGCGGGTTCACCCGCGAATACGAGGTGGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGaagaag               <  2:100012/149‑1 (MQ=255)
                                                                                                                              gAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGaagaag               >  5:74628/1‑149 (MQ=255)
                                                                                                                                  gggTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTg            >  6:246180/1‑148 (MQ=255)
                                                                                                                                    gTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAg         >  1:302232/1‑149 (MQ=255)
                                                                                                                                     ttCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGt        >  1:281283/1‑149 (MQ=255)
                                                                                                                                      tCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGt        >  5:268728/1‑148 (MQ=255)
                                                                                                                                        aCCCGCTAATCCGCGGGCCAACCCACCCCCGCATGCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcac     <  4:196166/149‑1 (MQ=255)
                                                                                                                                         cccGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcac     >  7:237250/1‑148 (MQ=255)
                                                                                                                                          ccGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcacca   >  4:69580/1‑149 (MQ=255)
                                                                                                                                           cgcgAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcaccac  >  2:324475/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
GCAGATCGATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCAC  >  NC_002947/5536912‑5537199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑