Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,537,076 A→T 17.4% intergenic (+53/+50) nadE → / ← PP_4870 NH(3)‑dependent NAD(+) synthetase/azurin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,537,0760AT17.4% 114.9 / 20.0 58intergenic (+53/+50)nadE/PP_4870NH(3)‑dependent NAD(+) synthetase/azurin
Reads supporting (aligned to +/- strand):  ref base A (22/23);  new base T (4/6);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 7.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.55e-01

TGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGAAGTGTC  >  NC_002947/5536929‑5537221
                                                                                                                                                   |                                                                                                                                                   
tGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTGACCCGCGGAAAAGCCGGTGAACCCACCGAc                                                                                                                                                    >  3:62607/1‑149 (MQ=255)
     ggacAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCGCCCGCGAATACGAGGGTGAACCCACCGACGCAt                                                                                                                                                >  6:349888/4‑148 (MQ=255)
      ggCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTc                                                                                                                                              >  2:247546/1‑149 (MQ=255)
         aGCCGGTTGACCAGGCTGCGTTCGACATCATCGTCGCCACGTCCCGCAAGACCCTGCACAAGCGCGAACTGCCGTTCGCTCATTCATAAGTGGCCTGCAGCAGCGGTTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTcgcg                                                                                                                                           <  8:17087/149‑1 (MQ=255)
          gccgcctGACCCGCCAGCGCTCGACATCACCCCCGCCCCCCACCGCACCACCCCCCACCCCCGCCACCTGCCGCTCGCTCCTTCATACGTGCCCTGCAGCAGCGGGTCCCCCCGCGAATACGAGGCTGAACCCACCGACGCATTCGCgg                                                                                                                                          <  8:312759/143‑1 (MQ=255)
          gCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                          <  8:40225/149‑1 (MQ=25)
          gCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                          <  6:184564/149‑1 (MQ=25)
          gCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCgg                                                                                                                                          <  1:339428/149‑1 (MQ=255)
          gCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAACTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCgg                                                                                                                                          <  6:210111/149‑1 (MQ=255)
            cGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGt                                                                                                                                        <  1:247546/149‑1 (MQ=255)
            cGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGt                                                                                                                                        <  7:6891/149‑1 (MQ=255)
                    cAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                          <  8:293970/139‑1 (MQ=21)
                    cAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                          >  7:293970/1‑139 (MQ=21)
                                                ccTACCGCAATACCCAGCACAAGCGCTAACTGCCTTTCGCTCCTTGATATGTGGCCTGCTGGAGCGGGTTCCCCCGCTAATACGCGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGa                                                                                                    <  1:114726/149‑1 (MQ=38)
                                                 cTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAACCCGCGAATACGAGGGTGTACCCACCGACGCATTAGCGGGTGAAACCGCTCCGGCAGGGGTCGCCGGCAGCCTTTGAt                                                                                                   >  1:100012/1‑149 (MQ=255)
                                                   aCCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCa                                                                                                            <  3:94060/138‑1 (MQ=255)
                                                     cGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACt                                                                                               >  3:240886/1‑149 (MQ=255)
                                                        aaGACCCACCAAACGCGCTAACTGCGGTTGGCTCTTTTATAAGTGGCTTGCAGGAGGGGGTTCACCCGCTAATACGTGGGTTAACCCACGGACGCTTTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGa                                                                                            <  6:120213/149‑1 (MQ=255)
                                                         aGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                          >  2:187165/1‑102 (MQ=14)
                                                         aGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                          <  1:192921/102‑1 (MQ=14)
                                                         aGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                          >  2:192921/1‑102 (MQ=14)
                                                         aGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGCCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                          <  1:187165/102‑1 (MQ=17)
                                                             ccAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                          >  6:155747/1‑98 (MQ=14)
                                                             ccAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                          <  5:155747/98‑1 (MQ=14)
                                                              cAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAACCCCCCAATACACGGGTGCACCCACCGACGCATTCGCGGGTGAACCCCCTACTGGAGGGTTGCCACTCAGCCGATGAATACTAGaagcaga                                                                                      >  2:291752/1‑143 (MQ=255)
                                                               aGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGTGGGTg                                                                                                                                       <  7:18906/99‑1 (MQ=255)
                                                               aGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGTGGGTg                                                                                                                                       >  8:18906/1‑99 (MQ=255)
                                                                  acaAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCtt                                                                                <  5:349888/149‑1 (MQ=255)
                                                                  acaAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAAACCGCGAAAAAGAGGGTGAAACCACCCACCCAATCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTGAGCCGTTGATTACTTTACGAT‑‑GAACtt                                                                                >  4:212607/1‑149 (MQ=255)
                                                                    aaGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGGGGGTGAACCCCCCGCCGCATTAGCGGGTGAACCCGCGCCTGGAGGGTTCGCCGGCCGCCTTTGATTACTTGAGGAT‑‑GACCTTgg                                                                              >  5:127438/1‑148 (MQ=255)
                                                                       cgcgAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGc                                                                                                                                <  8:251529/98‑1 (MQ=255)
                                                                       cgcgAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGc                                                                                                                                >  7:251529/1‑98 (MQ=255)
                                                                            aCTGCCGTTCCCTCCTTGATAAGGGGCCTCCCGGAGCGGGTTCCCCCGCGAATACGAGGGGGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTtcatc                                                                      <  6:127438/149‑1 (MQ=38)
                                                                                ccGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTTAACCAACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCg                                                                  <  7:316159/149‑1 (MQ=255)
                                                                                      gCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCTAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGAt                                                                                                   <  7:159944/112‑1 (MQ=255)
                                                                                               tAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATAAGAGGGTGAACCCACCGCCGCATTCGCGGGTGAACCCGCGCCGGGAGGGGTGGGCGGCAGCCGTTTATTACTTGACGAA‑‑GGCCGTGGCATTCATCATGGAGATTGGGCCCggg                                                    >  4:264725/1‑148 (MQ=19)
                                                                                                  gTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAgg                                                <  4:240886/149‑1 (MQ=255)
                                                                                                    ggCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTg                                              <  7:216306/149‑1 (MQ=255)
                                                                                                      catGCAGTAGCGGGTTCACCCGCTAATACGAGGGTGAACCCACCGACGCATTCGCGGGTTAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCa                                            <  3:212607/147‑1 (MQ=255)
                                                                                                           aGGAGCGGGTTAACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCagaag                                        >  2:111081/1‑148 (MQ=255)
                                                                                                             gAGCGGGTTCACCCGCGAATACGAGGTGGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGaagaag                                     <  2:100012/149‑1 (MQ=255)
                                                                                                             gAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGaagaag                                     >  5:74628/1‑149 (MQ=255)
                                                                                                                 gggTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTg                                  >  6:246180/1‑148 (MQ=255)
                                                                                                                   gTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAg                               >  1:302232/1‑149 (MQ=255)
                                                                                                                    ttCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGt                              >  1:281283/1‑149 (MQ=255)
                                                                                                                     tCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGt                              >  5:268728/1‑148 (MQ=255)
                                                                                                                       aCCCGCTAATCCGCGGGCCAACCCACCCCCGCATGCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcac                           <  4:196166/149‑1 (MQ=255)
                                                                                                                        cccGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcac                           >  7:237250/1‑148 (MQ=255)
                                                                                                                         ccGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcacca                         >  4:69580/1‑149 (MQ=255)
                                                                                                                          cgcgAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcaccac                        >  2:324475/1‑149 (MQ=255)
                                                                                                                                   gAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCa                                            >  2:138321/1‑120 (MQ=255)
                                                                                                                                   gAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCa                                            <  1:138321/120‑1 (MQ=255)
                                                                                                                                     gggTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGc             <  3:69580/149‑1 (MQ=255)
                                                                                                                                       gTGAACCCACCGACGCATTCGCGGGTGGACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCtt           >  7:129589/1‑149 (MQ=255)
                                                                                                                                           aCCCACCGACTCTTTCGCGGGGTAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGTATTCTTGTCGATTCTATTTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTg          <  1:291752/148‑1 (MQ=255)
                                                                                                                                            cccACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGAAg      >  5:134383/1‑149 (MQ=255)
                                                                                                                                              cACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGAAgtg    <  6:74628/149‑1 (MQ=255)
                                                                                                                                                 cGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGAAGTGTc  >  7:153579/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGAT‑‑GACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGAAGTGTC  >  NC_002947/5536929‑5537221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑