Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,538,573	A→T	13.4%	intergenic (+63/+58)	PP_4871 → / ← PP_4872	lysine decarboxylase family protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,538,573	0	A	T	13.4%	150.1 / 14.4	60	intergenic (+63/+58)	PP_4871/PP_4872	lysine decarboxylase family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (29/23);  new base T (4/4);  total (33/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.99e-01

GCAGCAGCCGGACGAGCTGCTGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGCAGCCTGCTGGTTACGC  >  NC_002947/5538426‑5538721                                                                                                                                                    |                                                                                                                                                     gcagcaGCCGGACGAGCTGCTGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTGCCACAAAGGCaa                                                                                                                                                     >  6:197554/1‑149 (MQ=255)   agcagcCGGACGAGCTGCTGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATg                                                                                                                                                   >  7:151765/1‑149 (MQ=255)      agcCGTACGATCGGCTGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTATGTGTGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCTGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATgggg                                                                                                                                                <  8:211279/149‑1 (MQ=39)             cGAGCTGCTGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGc                                                                                                                                         <  6:366137/149‑1 (MQ=255)              gAGCTGCTGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGcc                                                                                                                                        >  5:41914/1‑149 (MQ=255)                gctgctGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCtt                                                                                                                                      <  5:258048/149‑1 (MQ=255)                     tgGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTc                                                                                                                                 >  4:319022/1‑149 (MQ=255)                             aTGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATggg                                                                                                                         >  7:268353/1‑149 (MQ=255)                             aTGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCGTGTGTCGCGATggg                                                                                                                         >  5:228085/1‑149 (MQ=255)                                gACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTg                                                                                                                      <  3:319022/149‑1 (MQ=255)                                   aGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCa                                                                                                                    >  3:58709/1‑148 (MQ=255)                                    gCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCTACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCaaa                                                                                                                  <  4:48217/149‑1 (MQ=255)                                            gcgcCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCtt                                                                                                                                      >  4:256212/1‑121 (MQ=255)                                            gcgcCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCtt                                                                                                                                      <  3:256212/121‑1 (MQ=255)                                                cGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCaa                                                                                                        >  6:181065/1‑147 (MQ=255)                                                     gcgcCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGTCCCAATAGTCGa                                                                                                 >  4:139657/1‑149 (MQ=255)                                                     gcgcCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGa                                                                                                 >  6:321954/1‑149 (MQ=255)                                                     gcgcCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGa                                                                                                 >  6:316260/1‑149 (MQ=255)                                                      cgcCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATggg                                                                                                                         >  4:157145/1‑124 (MQ=255)                                                      cgcCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATggg                                                                                                                         <  3:157145/124‑1 (MQ=255)                                                       gcCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGAt                                                                                                >  1:213037/1‑148 (MQ=255)                                                        ccTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCa                                                                                              <  5:181065/149‑1 (MQ=255)                                                             gTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGTCCCAATAGTCGATCAGTTaa                                                                                         <  3:139657/149‑1 (MQ=255)                                                                     aCAAGCCCCCTGACTGACGCTGAGACTTTTGGGACCGCTTCGGGGTCCTTTCGCGACACAAGCCCCCCCCCACAACGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAAt                                                                                 <  8:151765/149‑1 (MQ=38)                                                                     aCAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCCCATTGCCTTTGTGGGAGCGGCCTTGTGTCGCGaaa                                                                                                                           >  7:216288/1‑105 (MQ=18)                                                                     aCAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCCCATTGCCTTTGTGGGAGCGGCCTTGTGTCGCGaaa                                                                                                                           >  7:221956/1‑105 (MQ=18)                                                                     aCAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCCCATTGCCTTTGTGGGAGCGGCCTTGTGTCGCGaaa                                                                                                                           <  8:216288/107‑3 (MQ=18)                                                                     aCAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCCCATTGCCTTTGTGGGAGCGGCCTTGTGTCGCGaaa                                                                                                                           <  8:221956/107‑3 (MQ=18)                                                                     aCAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAAt                                                                                 <  5:321954/149‑1 (MQ=255)                                                                     aCAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAAt                                                                                 <  5:316260/149‑1 (MQ=255)                                                                         gcagcCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGa                                                                               >  8:65644/1‑147 (MQ=255)                                                                            gcCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGc                                                                          <  2:213037/149‑1 (MQ=255)                                                                                          gagaCTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGcttctt                                                            >  7:12998/1‑149 (MQ=255)                                                                                           agaCTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCttg                                                           <  8:268353/149‑1 (MQ=255)                                                                                                gaaaggACCGCGAAGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGcc                                                      >  3:94961/5‑149 (MQ=255)                                                                                                  tGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGccc                                                     <  7:65644/148‑1 (MQ=255)                                                                                                       ccGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGACCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCa                                               >  4:268420/1‑149 (MQ=255)                                                                                                              gcgGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGACCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGc                                        <  3:268420/149‑1 (MQ=255)                                                                                                                ggTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTc                                      >  1:216903/1‑149 (MQ=255)                                                                                                                 gTCCTTTCGCGACACAAGGCCGCTCCCACAAATGCAATGGGGCAGAGGCCTTTTGTCGCCATGGGCAGCAAAGCAGCCCCAAAATTCGATCAGTAAACGCGGAAAGACAGGCTGACGCGGCGTCAAGATGCCCGTCCCACCCTTGCACg                                     >  2:249604/1‑149 (MQ=255)                                                                                                                     tttCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCctc                                 >  1:314115/1‑149 (MQ=255)                                                                                                                     tttCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCctc                                 >  2:215579/1‑149 (MQ=255)                                                                                                                        cgcgACACACGGCCGCTCCCACAACGGCAATGGGGGAGGGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCtt                               <  6:228085/148‑1 (MQ=255)                                                                                                                         gcgACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTg                             >  5:4928/1‑149 (MQ=255)                                                                                                                          cgACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTgc                            <  8:12998/149‑1 (MQ=255)                                                                                                                           gACACAAGGCCGCTCCCACAAAGGCAATGGGGGCGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTgcg                           <  4:58709/149‑1 (MQ=255)                                                                                                                            acacAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTgcgc                          >  8:268688/1‑149 (MQ=255)                                                                                                                              acaAGGCCGCTCCCCCATTGCCTTTGTGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAg                                                                            >  2:262617/1‑97 (MQ=18)                                                                                                                              acaAGGCCGCTCCCCCATTGCCTTTGTGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAg                                                                            <  1:262617/97‑1 (MQ=18)                                                                                                                                aaGGCCGCTCCCCCATTGCCTTTGTGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCAccc                                            <  3:29491/127‑1 (MQ=37)                                                                                                                                aaGGCCGCTCCCCCATTGCCTTTGTGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCAccc                                            >  4:29491/1‑127 (MQ=37)                                                                                                                                 cggCCCCTCCCACAAAGGCATTGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCtt                     <  8:214227/148‑1 (MQ=255)                                                                                                                                   gCCGCTCCCACAACGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTgg                   <  6:285440/149‑1 (MQ=255)                                                                                                                                     cGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTgg                   >  4:91602/1‑147 (MQ=255)                                                                                                                                       cTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGCAGc               >  2:155410/1‑149 (MQ=255)                                                                                                                                       cTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGCAGc               >  6:229956/1‑149 (MQ=255)                                                                                                                                          ccACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGCAGcc              >  3:246800/1‑147 (MQ=255)                                                                                                                                          ccACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGCAGcc              <  4:246800/147‑1 (MQ=255)                                                                                                                                           cacaAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGCAGCctgc           >  1:354168/1‑149 (MQ=255)                                                                                                                                           cacaAAGGCAATGCGGGAGCGGCCTTGTGTCGCGATGGGCTGCATAGCAGCACCAATAGTCGATCAGTTAACGCGGAATGACAGTCTGACGCGGCTTCTTGTTGCCCTTGCCCCCCTTGCTCGCCTCTTTGCGATCCTTGGCAGCctgc           >  7:125965/1‑149 (MQ=39)                                                                                                                                             caAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGCAGCctgctg         <  6:4928/149‑1 (MQ=255)                                                                                                                                                    aaTGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGCAGCCTGCTGGTTAcgc  <  1:215579/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GCAGCAGCCGGACGAGCTGCTGGAAGGGATGGACAGCTTTGTGGCGCCGGTCGCGCCTAAGTGGGTCGACAAGCAGCCTGACTGAAGCTGAGACTTTTGGGACCGCTTCGCGGTCCTTTCGCGACACAAGGCCGCTCCCACAAAGGCAATGGGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGCCCCAATAGTCGATCAGTTAACGCGGAATGACAGGCTGACGCGGCTTCTTGTTGCCCTTGCCACCCTTGCTCGCCTCTTTGCGCTCCTTGGCAGCCTGCTGGTTACGC  >  NC_002947/5538426‑5538721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑