Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,550,672	T→A	7.8%	L305Q (CTG→CAG)	PP_4881 →	iron ABC transporter substrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,550,672	0	T	A	7.8%	172.8 / 4.7	64	L305Q (CTG→CAG)	PP_4881	iron ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (30/29);  new base A (3/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCC  >  NC_002947/5550526‑5550820                                                                                                                                                    |                                                                                                                                                         cGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑g                                                                                                                                                        >  2:15665/1‑148 (MQ=255) cGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑Ga                                                                                                                                                       <  6:129739/149‑1 (MQ=255)       aGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGt                                                                                                                                                 <  1:277547/149‑1 (MQ=255)        ggCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGACAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGtt                                                                                                                                                <  1:15665/149‑1 (MQ=255)           gTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTTGATGGCCAGCTAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAATCCGTTcg‑‑‑a                                                                                                                                          <  6:82025/149‑1 (MQ=255)           gTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTcg‑‑‑a                                                                                                                                          <  3:128615/149‑1 (MQ=255)           gTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATAACCCAGACAACCGCCGAGTACACACTGCACAAGGCCATGGTTTCAGGCCGCGGACA‑GAAAccatgca                                                                                                                                              >  7:69634/1‑143 (MQ=255)              gTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCAGGCCGCGGTCA‑GAAAccatgc                                                                                                                                               <  2:277085/144‑5 (MQ=255)              gTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCAGGCCGCGGTCA‑GAAAccatgc                                                                                                                                               >  1:277085/1‑140 (MQ=255)                     ccAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCACACCACCGCCGACTACCCAATGCACAACGGCATGGTTTCTGACCGCCGCCT‑GAAACCGTTCG‑‑‑ACGAGCTgcgc                                                                                                                                >  3:47495/1‑149 (MQ=255)                                 cGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGGAAACCGTTCG‑‑‑ACGAGCTGCGCccgccg                                                                                                                          >  5:237600/1‑144 (MQ=255)                                 cGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGAtc                                                                                                                    >  1:83148/1‑149 (MQ=255)                                  gAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGCCCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGAtct                                                                                                                   >  7:139012/1‑149 (MQ=255)                                   aaaGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCAGCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGAtctc                                                                                                                  >  3:276028/1‑149 (MQ=255)                                    aagaagCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCg                                                                                                                 <  2:338766/149‑1 (MQ=255)                                       aagCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGTCCGCGGCCT‑GAAACCGTTcg‑‑‑ac                                                                                                                                         <  2:320942/122‑1 (MQ=255)                                       aagCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTcg‑‑‑ac                                                                                                                                         >  1:320942/1‑122 (MQ=255)                                          cccAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCt                                                                                                           <  8:139012/149‑1 (MQ=255)                                            cAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGa                                                                                                         >  3:104649/1‑149 (MQ=255)                                             aGGTCCTGCTCATCTGGATGGCCTGCGATGAAGGC‑CAGCGCGTGATGACCGAGACCACCGCCGAGTTCCCACTGCACAAGGGCATGGTTTCTGAGCGCTGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGGCTGCTGAc                                                                                                        <  4:47495/149‑1 (MQ=255)                                             aGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCACAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTcg‑‑‑a                                                                                                                                          >  1:53830/1‑116 (MQ=255)                                             aGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTcg‑‑‑a                                                                                                                                          <  2:53830/115‑1 (MQ=255)                                               gCCCTGCTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTCATCACCCAGACCACCGCCGAGTACCCACTGCACAACGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCt                                                                                                      >  8:262787/1‑149 (MQ=255)                                                  cTGCTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTggg                                                                                                   <  2:83148/149‑1 (MQ=255)                                                     cTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGGAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTggg                                                                                                   <  6:237600/147‑1 (MQ=255)                                                     cTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCaa                                                                                                >  2:111939/1‑149 (MQ=255)                                                        aaCTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCAGGCCGCGGTCA‑GAAAccatgc                                                                                                                                               <  2:210644/102‑5 (MQ=38)                                                        aaCTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCAGGCCGCGGTCA‑GAAAccatgc                                                                                                                                               >  1:210644/1‑98 (MQ=38)                                                             gATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAg                                                                                         >  3:309601/1‑148 (MQ=255)                                                                   cAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCa                                                                                  <  3:353362/149‑1 (MQ=255)                                                                           aagGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTCGGCATTGCCCAggt                                                                                       >  6:39659/1‑135 (MQ=255)                                                                           aagGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTCGGCATTGCCCAggt                                                                                       <  5:39659/136‑2 (MQ=255)                                                                             ggC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGa                                                                        <  5:307929/149‑1 (MQ=255)                                                                                 cAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCGCCCTCGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGa                                                                        <  1:111939/149‑1 (MQ=255)                                                                                         gATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCg                                                             >  7:112057/1‑149 (MQ=255)                                                                                          aTCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCg                                                             <  4:309601/148‑1 (MQ=255)                                                                                               ccAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGc                                                       >  5:363218/1‑149 (MQ=255)                                                                                                     caccGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCtgat                                                 <  7:262787/149‑1 (MQ=255)                                                                                                     caccGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCtgat                                                 >  7:85169/1‑149 (MQ=255)                                                                                                                  ccACTGCACAAGGGCATGGTTTCAGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGCCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTCCTCTGATGACTCCCGCCCTg                                    >  6:28781/1‑149 (MQ=255)                                                                                                                   cACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGt                                   <  8:112057/149‑1 (MQ=255)                                                                                                                    aCTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTc                                  <  4:276028/149‑1 (MQ=255)                                                                                                                    aCTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTc                                  >  1:210523/1‑149 (MQ=255)                                                                                                                         acaAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAAc                                                                            >  7:105675/1‑102 (MQ=255)                                                                                                                         acaAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAAc                                                                            <  8:105675/102‑1 (MQ=255)                                                                                                                         acaAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAAc                             >  1:232070/1‑149 (MQ=255)                                                                                                                         acaAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAAc                             >  7:121457/1‑149 (MQ=255)                                                                                                                            aGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCgg                          >  2:79268/1‑149 (MQ=255)                                                                                                                               gCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGc                       <  6:363218/149‑1 (MQ=255)                                                                                                                               gCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGc                       >  6:371555/1‑149 (MQ=255)                                                                                                                                 aTGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCg                     >  6:204196/1‑149 (MQ=255)                                                                                                                                  tGGTTTGTGACCGCGGCGT‑GATACCGTTCG‑‑‑ACGAGCTGCGCCGGCCGTAGATCTGGCCAGCTGACCTGTGCAATGCCGAGGAAGCCATGGAACTTGTGGGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGTAACGGTGcag                     <  4:104649/148‑3 (MQ=255)                                                                                                                                  tGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGGCGAAGATCTCGCCAGCTGACCTGGGCAATGGCGAGGTAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCtgat                                                 <  1:142433/120‑1 (MQ=255)                                                                                                                                   ggTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCgg                    >  1:213035/1‑148 (MQ=255)                                                                                                                                         tGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCtt             >  7:173905/1‑149 (MQ=255)                                                                                                                                         tGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCtt             >  7:86610/1‑149 (MQ=255)                                                                                                                                         tGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCtt             >  7:91560/1‑149 (MQ=255)                                                                                                                                           aCCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGc                                                                      <  5:182357/90‑1 (MQ=255)                                                                                                                                           aCCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGc                                                                      >  6:182357/1‑90 (MQ=255)                                                                                                                                            ccGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGt                   >  7:129826/1‑140 (MQ=255)                                                                                                                                            ccGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGt                   <  8:129826/140‑1 (MQ=255)                                                                                                                                              gcgGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTAc        <  2:232070/149‑1 (MQ=255)                                                                                                                                                  ccT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAg    <  8:85169/149‑1 (MQ=255)                                                                                                                                                   cT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGc   <  8:173905/149‑1 (MQ=255)                                                                                                                                                    t‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGAAGCGTACCggcac  >  5:336995/1‑145 (MQ=255)                                                                                                                                                    |                                                                                                                                                         CGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGC‑CAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCT‑GAAACCGTTCG‑‑‑ACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCC  >  NC_002947/5550526‑5550820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑