Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,659,686 A→T 7.3% T244S (ACT→TCT) ‡ metK → methionine adenosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,659,6860AT 7.3% 220.7 / 3.3 77T244S (ACT→TCT) ‡metKmethionine adenosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (38/33);  new base T (3/3);  total (41/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGAAAGACCTGCAAGAAGCCGTGATGGAGCTGATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACATCGTCGCCGCCGGTT  >  NC_002947/5659544‑5659830
                                                                                                                                              |                                                                                                                                                
gCAGAAAGACCTGCAAGAAGCCGTGATGGAGCTGATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGcc                                                                                                                                            >  5:326316/1‑149 (MQ=255)
    aaaGACCTGCAAGAAGCCGTGATGGAGCTGATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCaa                                                                                                                                        <  6:326316/149‑1 (MQ=255)
             cAAGAAGCCGTGATGGAGCTGATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGatcatc                                                                                                                                 <  3:159868/147‑1 (MQ=255)
                  agCCGTGATGGAGCTGATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGcc                                                                                                                                            <  4:350917/131‑1 (MQ=255)
                         aTGGAGCTGATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACgg                                                                                                                   >  8:75702/1‑149 (MQ=255)
                           ggAGCTGATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTg                                                                                                                 >  1:25227/1‑149 (MQ=255)
                            gAGCTGATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTg                                                                                                                 <  7:315179/148‑1 (MQ=255)
                            gAGCTGATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTg                                                                                                                 <  6:93292/148‑1 (MQ=255)
                                   tCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGcc                                                                                                         <  2:358572/149‑1 (MQ=255)
                                    cGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCAGTCAGGCCGCag                                                                                                                                        <  1:37457/117‑2 (MQ=255)
                                    cGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCAGTCAGGCCGCag                                                                                                                                        >  2:37457/1‑116 (MQ=255)
                                      tCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGt                                                                                                      >  7:134882/1‑149 (MQ=255)
                                          gCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGatcatc                                                                                                                                 <  1:299876/118‑1 (MQ=255)
                                          gCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGatcatc                                                                                                                                 >  2:299876/1‑118 (MQ=255)
                                           cacaCGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACgg                                                                                                 >  8:258453/1‑149 (MQ=255)
                                            acacGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCAGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACggg                                                                                                <  1:41684/149‑1 (MQ=255)
                                             cacGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACgggg                                                                                               <  7:75702/149‑1 (MQ=255)
                                                    ccggccgAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGgcgc                                                                                        <  7:308488/149‑1 (MQ=255)
                                                       gccgAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGtt                                                                                     <  2:119038/149‑1 (MQ=255)
                                                         cgAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACgggg                                                                                               >  3:143876/1‑137 (MQ=255)
                                                         cgAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACgggg                                                                                               <  4:143876/137‑1 (MQ=255)
                                                                ctGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCaa                                                                            <  2:270488/149‑1 (MQ=255)
                                                                ctGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCaa                                                                            <  7:258453/149‑1 (MQ=255)
                                                                 tGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAg                                                                           >  2:264640/1‑149 (MQ=255)
                                                                  gCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGTCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAg                                                                           >  6:226711/1‑148 (MQ=255)
                                                                   cacaAAGGCAGCCAGTACGACATCTACTGGACCGGGCACTTCATCATGGGTGGGCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTg                                                                                                                 <  4:279733/109‑1 (MQ=255)
                                                                   cacaAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCAACGGTg                                                                                                                 >  3:279733/1‑109 (MQ=255)
                                                                    acaAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGAc                                                                        >  6:107254/1‑149 (MQ=255)
                                                                      aaaGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGAccc                                                                      >  8:73532/1‑149 (MQ=255)
                                                                        aGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGt                                                                    <  5:107254/149‑1 (MQ=255)
                                                                          gCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTc                                                                   >  5:193974/1‑148 (MQ=255)
                                                                           cACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTcc                                                                  >  5:163423/1‑148 (MQ=255)
                                                                             cccAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGa                                                                         <  4:321388/139‑1 (MQ=255)
                                                                             cccAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGa                                                                         >  3:321388/1‑139 (MQ=255)
                                                                              ccAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGcgcg                                                                                       <  3:244302/124‑1 (MQ=255)
                                                                              ccAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGcgcg                                                                                       >  4:244302/1‑124 (MQ=255)
                                                                              ccAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAgg                                                              >  4:324611/1‑149 (MQ=255)
                                                                              ccAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAgg                                                              >  5:270103/1‑149 (MQ=255)
                                                                               cAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTCACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGATCTCCGGCAAGGACCCGTCCAAGGt                                                             >  3:220923/1‑149 (MQ=255)
                                                                                 gTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTc                                                            >  4:116771/1‑148 (MQ=255)
                                                                                 gTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCg                                                           >  7:25542/1‑149 (MQ=255)
                                                                                    ccACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGAc                                                         <  3:116771/148‑1 (MQ=255)
                                                                                        aTCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTc                                                    >  5:330575/1‑149 (MQ=255)
                                                                                        aTCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCACCGGCAAGGACCCGTCCAAGGTCGACCGTTc                                                    >  8:27492/1‑149 (MQ=255)
                                                                                              ccGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGc                                              <  7:73532/149‑1 (MQ=255)
                                                                                              ccGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCAAAGGTCGACCGTTCCGCTGc                                              <  2:25227/149‑1 (MQ=255)
                                                                                                gACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGTCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCt                                            <  5:226711/149‑1 (MQ=255)
                                                                                                    ggCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACgc                                        >  2:105743/1‑149 (MQ=255)
                                                                                                     gCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACgcc                                       >  8:276804/1‑149 (MQ=255)
                                                                                                          ttCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACgccggccg                                  >  3:92064/1‑149 (MQ=255)
                                                                                                            catcatCGGTGGCCCGGTTGGTGACTGCGGCGTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGc                                 <  4:220923/148‑1 (MQ=255)
                                                                                                            catcatCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAg                                                                           >  1:77596/1‑106 (MQ=255)
                                                                                                            catcatCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAg                                                                           <  2:77596/106‑1 (MQ=255)
                                                                                                             atcatcGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCt                                <  7:276804/148‑1 (MQ=255)
                                                                                                              tcatcGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTAc                              <  6:193974/149‑1 (MQ=255)
                                                                                                              tcatcGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTAc                              >  6:233186/1‑149 (MQ=255)
                                                                                                                atcGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTAc                                                                                                                     <  8:187606/60‑1 (MQ=255)
                                                                                                                atcGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTAc                                                                                                                     >  7:187606/1‑60 (MQ=255)
                                                                                                                    gTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGcc                        <  8:25542/149‑1 (MQ=255)
                                                                                                                         ccGGTGCGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGaa                   >  1:83230/1‑149 (MQ=255)
                                                                                                                           ggtgggtgACTGCGGTCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAGCa                 >  5:275374/1‑149 (MQ=255)
                                                                                                                             tgggtgACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCACCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACAtc               <  7:27492/149‑1 (MQ=255)
                                                                                                                              gggtgACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGccc                                                        >  6:95113/1‑107 (MQ=255)
                                                                                                                                gtgACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGc                                              <  3:335859/115‑1 (MQ=255)
                                                                                                                                gtgACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGc                                              >  4:335859/1‑115 (MQ=255)
                                                                                                                                  tcttGCGGCCAGTCAGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGc                         >  5:182537/4‑134 (MQ=255)
                                                                                                                                  tcttGCGGCCAGTCAGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGc                         <  6:182537/131‑1 (MQ=255)
                                                                                                                                  gACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTgg                          <  2:99707/133‑1 (MQ=255)
                                                                                                                                  gACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTgg                          >  1:99707/1‑133 (MQ=255)
                                                                                                                                  gACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACATCGTcgc          >  2:62573/1‑149 (MQ=255)
                                                                                                                                    ttGCGGCCAGTCAGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTtctc                                                                                  <  2:127483/74‑1 (MQ=255)
                                                                                                                                    ttGCGGCCAGTCAGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTtctc                                                                                  >  1:127483/2‑75 (MQ=255)
                                                                                                                                       cGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACATCGTCGccgccg     >  8:1758/1‑149 (MQ=255)
                                                                                                                                       cGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACATCGTCGccgccg     >  8:319205/1‑149 (MQ=255)
                                                                                                                                        ggCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACATCGTCGCCGCCgg    >  8:259653/1‑149 (MQ=255)
                                                                                                                                          ccTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACATCGTCGCCGCCGGtt  <  6:330575/149‑1 (MQ=255)
                                                                                                                                          ccTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACATCGTCGCCGCCGGtt  <  3:324611/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                
GCAGAAAGACCTGCAAGAAGCCGTGATGGAGCTGATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACATCGTCGCCGCCGGTT  >  NC_002947/5659544‑5659830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑