Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,672,372	G→C	6.9%	G208R (GGC→CGC)	PP_4979 →	substrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,672,372	0	G	C	6.9%	183.2 / 3.3	58	G208R (GGC→CGC)	PP_4979	substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (28/26);  new base C (2/2);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01

CTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGAGCAGCAG  >  NC_002947/5672233‑5672518                                                                                                                                             |                                                                                                                                                   cTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCACCAGGCCCAGGTACTGCGCCTggcgg                                                                                                                                           <  7:341323/149‑4 (MQ=255)  ttCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTgcg                                                                                                                                          >  6:92438/1‑149 (MQ=255)      gCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAg                                                                                                                                      >  5:127597/1‑149 (MQ=255)       cAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGc                                                                                                                                     >  1:14536/1‑149 (MQ=255)          gCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTg                                                                                                                                  <  7:181467/149‑1 (MQ=255)           cTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTgg                                                                                                                                 <  4:72218/149‑1 (MQ=255)            tGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTggg                                                                                                                                >  2:168593/1‑149 (MQ=255)             gATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGgc                                                                                                                               >  4:196549/1‑149 (MQ=255)               tACTGGGACGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGgcgc                                                                                                                             <  4:198786/149‑1 (MQ=255)                 cTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGcgcg                                                                                                                            <  6:96866/148‑1 (MQ=255)                 cTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGcgcg                                                                                                                            >  5:96866/1‑148 (MQ=255)                      ccGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCAGGGCCTGGTGCGCCAGCCTGGGCGCGAGGCAc                                                                                                                      >  5:222043/1‑149 (MQ=255)                         cATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTgg                                                                                                                   <  2:185684/149‑1 (MQ=255)                         cATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTgg                                                                                                                   >  3:159973/1‑149 (MQ=255)                                   gTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAGCAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGAGAGGCACTGGCCGTGGCCt                                                                                                          <  6:152231/148‑1 (MQ=255)                                      aTCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTg                                                                                                       <  4:159973/148‑1 (MQ=255)                                      aTCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTg                                                                                                       <  5:272429/148‑1 (MQ=255)                                         accgaccgCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCg                                                                                                    >  7:308724/1‑148 (MQ=255)                                          ccgaccgCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGCTAAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCg                                                                                                    >  1:269854/1‑148 (MQ=255)                                           cgaccgCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCg                                                                                                    >  2:42367/1‑146 (MQ=255)                                            gaccgCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGaa                                                                                                  >  6:343950/1‑147 (MQ=255)                                                 cAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGt                                                                                                               <  8:334796/129‑1 (MQ=255)                                                 cAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGt                                                                                                               >  7:334796/1‑129 (MQ=255)                                                 cAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGc                                                                                           <  5:92438/149‑1 (MQ=255)                                                           cATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCaa                                                                                 <  8:308724/149‑1 (MQ=255)                                                               aCCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCa                                                                              <  6:127597/148‑1 (MQ=255)                                                                ccTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGc                                                                                              >  1:167689/1‑131 (MQ=255)                                                                ccTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGc                                                                                              <  2:167689/131‑1 (MQ=255)                                                                ccTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCg                                                                                       <  1:154055/138‑1 (MQ=255)                                                                ccTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCg                                                                                       >  2:154055/1‑138 (MQ=255)                                                                ccTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAg                                                                             <  5:129170/148‑1 (MQ=255)                                                                ccTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAg                                                                             <  1:42367/148‑1 (MQ=255)                                                                ccTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAg                                                                             >  6:129170/1‑148 (MQ=255)                                                                   gCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGACGAAGAGCTGCAGCGGTTCAAGCAGGAGc                                                                         >  7:217856/1‑149 (MQ=255)                                                                          cAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTa                                                                  >  3:211810/1‑149 (MQ=255)                                                                             cTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGc                                                               <  2:269854/149‑1 (MQ=255)                                                                                  ccTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGa                                                          >  7:136054/1‑149 (MQ=255)                                                                                        aCAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGc                                                     >  2:250609/1‑148 (MQ=255)                                                                                        aCAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGc                                                     >  8:86859/1‑148 (MQ=255)                                                                                              aaGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGt                                               <  1:168593/149‑1 (MQ=255)                                                                                                                     aTCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACAt                        >  7:135366/1‑149 (MQ=255)                                                                                                                      tCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACAtt                       <  4:211810/149‑1 (MQ=255)                                                                                                                      tCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACAtt                       <  7:86859/149‑1 (MQ=255)                                                                                                                        gcgcaccAGGCCCAGGTACTGCGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATtct                     >  2:41475/6‑149 (MQ=255)                                                                                                                          gcaccAGGCCCAGGTACTGCGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATtctc                    >  6:158956/4‑148 (MQ=255)                                                                                                                          ccgccAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATtctc                    >  3:231615/1‑148 (MQ=255)                                                                                                                           cgccAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCaa                  >  5:354111/1‑149 (MQ=255)                                                                                                                            gccAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAAc                 <  3:196549/149‑1 (MQ=255)                                                                                                                               aGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGcc              >  8:205602/1‑149 (MQ=255)                                                                                                                                 gcgcAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACAtt                       >  1:151928/1‑138 (MQ=255)                                                                                                                                 gcgcAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACAtt                       <  2:151928/138‑1 (MQ=255)                                                                                                                                  cgcAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGtt           >  4:76183/1‑149 (MQ=255)                                                                                                                                  cgcAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGtt           <  2:9304/149‑1 (MQ=255)                                                                                                                                   gcAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGtt           >  8:132558/1‑148 (MQ=255)                                                                                                                                     aggtacTGCGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGag        <  1:41475/144‑1 (MQ=255)                                                                                                                                     aGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGag        <  6:354111/149‑1 (MQ=255)                                                                                                                                         ccTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTACAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGagcagc    <  2:14536/149‑1 (MQ=255)                                                                                                                                          cTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGAgcagca   <  5:343950/149‑1 (MQ=255)                                                                                                                                             ggCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGAGcagcag  <  3:76183/147‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                   CTTCGGCAAGCTGATACTGGGCCGCATCGACCTGGTGATCACCGACCGCAGGGTGGGGCATTACCTGCTCCGCCAGCTCGGCCTGCAACAGC‑AAGTCGAAGAACTGCCGCTGGTAATCAGCCGCCAGGCGCAGTACCTGGGCCTGGTGCGCAAGCCTGGGCGCGAGGCACTGGCCGTGGCCTTTGCCGAAGAGCTGCAGCGGTTCAAGCAGGAGCCGGCCTATGCAGCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGAGCAGCAG  >  NC_002947/5672233‑5672518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑