Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,725,838 G→A 7.7% T376T (ACC→ACT)  opgH ← glucans biosynthesis glucosyltransferase H

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,725,8380GA 7.7% 145.2 / 2.6 52T376T (ACC→ACT) opgHglucans biosynthesis glucosyltransferase H
Reads supporting (aligned to +/- strand):  ref base G (26/22);  new base A (2/2);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01

ATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCATGTACTTGTACTCGCCACCCCAGCGACGGCAGAAGTCGTCGAGGTTACCG  >  NC_002947/5725692‑5725985
                                                                                                                                                  |                                                                                                                                                   
aTGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGt                                                                                                                                                   <  1:258807/149‑1 (MQ=255)
aTGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGt                                                                                                                                                   >  5:98844/1‑149 (MQ=255)
         atCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGgatgat                                                                                                                                          >  4:149506/1‑149 (MQ=255)
             cgTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCg                                                                                                                                      <  1:109274/149‑1 (MQ=255)
                      cccAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCggg                                                                                                                             >  7:162553/1‑149 (MQ=255)
                       ccAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGt                                                                                                                            >  7:9568/1‑149 (MQ=255)
                         agtagtGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTg                                                                                                                          <  4:118696/149‑1 (MQ=255)
                                      aCCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAgg                                                                                                              <  3:149506/148‑1 (MQ=255)
                                        ccAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATc                                                                                                                 <  3:190448/143‑1 (MQ=255)
                                        ccAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATc                                                                                                                 >  4:190448/1‑143 (MQ=255)
                                               ccAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTg                                                                                                                          <  2:326458/127‑1 (MQ=255)
                                               ccAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTg                                                                                                                          >  1:326458/1‑127 (MQ=255)
                                                   aaGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGgctgct                                                                                                >  5:75090/1‑149 (MQ=255)
                                                             cGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTcg                                                                                      >  6:117081/1‑149 (MQ=255)
                                                                       cAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGa                                                                            >  2:135759/1‑149 (MQ=255)
                                                                       cAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGa                                                                            <  6:98844/149‑1 (MQ=255)
                                                                        aGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTc                                                                                               >  7:303146/1‑129 (MQ=255)
                                                                        aGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTc                                                                                               <  8:303146/129‑1 (MQ=255)
                                                                         gTGGGCCGTACACGCGGGTAGCGAACTGCTGGATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGCCACTCGCCGCTCATGACg                                                                          <  4:270858/149‑1 (MQ=255)
                                                                            ggCCGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTg                                                                       >  1:161564/1‑149 (MQ=255)
                                                                              ccGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTTCTCAGGCACTCGCCGCTCATGACGccga                                                                      >  3:80016/1‑145 (MQ=255)
                                                                              ccGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTc                                                                     >  3:113574/1‑149 (MQ=255)
                                                                               cGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCg                                                                    <  8:127281/149‑1 (MQ=255)
                                                                                 tACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGc                                                                  >  3:253542/1‑149 (MQ=255)
                                                                                    acGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTc                                                               <  5:117081/149‑1 (MQ=255)
                                                                                    acGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTc                                                               >  8:185986/1‑149 (MQ=255)
                                                                                           tAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTTCTCAGGCACTCGCCGCTCATGACGCCGACAGCGTcatgagcg                                                        <  4:80016/149‑8 (MQ=255)
                                                                                               gAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCa                                                    >  6:362128/1‑149 (MQ=255)
                                                                                                  accctgtATGCACGCATGCAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCAtg                                                  >  3:204631/4‑148 (MQ=255)
                                                                                                   tgctgcATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCAtgta                                                >  5:146685/1‑149 (MQ=255)
                                                                                                    gctgcATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCAGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGCCCAtgtac                                               >  4:59762/1‑149 (MQ=255)
                                                                                                    gctgcATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCAGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCAtgtac                                               >  4:64453/1‑149 (MQ=255)
                                                                                                      tgcATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCAtgtactt                                             >  3:347880/1‑149 (MQ=255)
                                                                                                        cATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGTCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCAtgtacttg                                            >  5:102889/1‑148 (MQ=255)
                                                                                                         aTGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCAtgtacttgta                                          >  7:273962/1‑149 (MQ=255)
                                                                                                          tGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCAtgtacttgtac                                         <  4:113574/149‑1 (MQ=255)
                                                                                                          tGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCAtgtacttgtac                                         <  2:161564/149‑1 (MQ=255)
                                                                                                            cGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCATGTACTTGTACTc                                       <  4:206650/149‑1 (MQ=255)
                                                                                                                   aCAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCATGTACTTGTACTCGCCAccc                                >  5:201232/1‑149 (MQ=255)
                                                                                                                       ggTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCATGTACTTGTACTCGCCACCCCAGc                            >  6:38657/1‑149 (MQ=255)
                                                                                                                           tCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCATGTACTTGTACTCGCCACCCCAGCGAc                         <  5:38657/148‑1 (MQ=255)
                                                                                                                           tCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCATGTACTTGTACTCGCCACCCCAGCGAc                         <  8:162553/148‑1 (MQ=255)
                                                                                                                           tCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCATGTACTTGTACTCGCCACCCCAGCGACg                        <  8:9568/149‑1 (MQ=255)
                                                                                                                           tCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCATGTACTTGTACTCGCCACCCCAGCGACg                        <  8:273962/149‑1 (MQ=255)
                                                                                                                             cATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCATGTACTTGTACTc                                       >  6:168722/1‑132 (MQ=255)
                                                                                                                             cATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCATGTACTTGTACTc                                       <  5:168722/132‑1 (MQ=255)
                                                                                                                                 cccGAGTCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCATGTACTTGTACTCGCCACCCCAGCGACGGCAGaa                  <  5:362128/149‑1 (MQ=255)
                                                                                                                                         tttCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCATGTACTTGTACTCGCCACCCCAGCGACGGCAGAAGTCGTCGa          <  4:253542/149‑1 (MQ=255)
                                                                                                                                               cccAGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCATGTACTTGTACTCGCCACCCCAGCGACGGCAGAAGTCGTCGAGGTTAc    <  3:59762/149‑1 (MQ=255)
                                                                                                                                               cccAGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCATGTACTTGTACTCGCCACCCCAGCGACGGCAGAAGTCGTCGAGGTTAc    <  3:64453/149‑1 (MQ=255)
                                                                                                                                                ccGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCATGTACTTGTACTCGCCACCCCAGCGACGGCAGAAGTCGTCGAGGTTAcc   >  5:228192/1‑149 (MQ=255)
                                                                                                                                                 cGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCATGTACTTGTACTCGCCACCCCAGCGACGGCAGAAGTCGTCGAGGTTACCg  >  1:42810/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACTGCTGCATGCGTGCATACAGGGTGTCCATGCCCGAGGCTTTCGGCCCGGTCTGGATGATGCCGGCGTCCGGGTTGGCCTCCATCAGGCGCACCAGGCTGCTCAGGCACTCGCCGCTCATGACGCTGTCGGCGTCGAGCACGACCATGTACTTGTACTCGCCACCCCAGCGACGGCAGAAGTCGTCGAGGTTACCG  >  NC_002947/5725692‑5725985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑