| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,740,470 | A→T | 15.2% | intergenic (+35/+36) | hutF → / ← PP_5037 | formiminoglutamate deiminase/lipocalin family lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,740,470 | 0 | A | T | 15.2% | 69.5 / 5.7 | 38 | intergenic (+35/+36) | hutF/PP_5037 | formiminoglutamate deiminase/lipocalin family lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (13/18); new base T (3/3); total (17/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
GGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGC‑AACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGG > NC_002947/5740324‑5740613 | ggTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTTCAGCCCATAGCGTGCCAg > 5:133354/1‑149 (MQ=255) cgcgATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGccc < 6:133944/148‑1 (MQ=255) gATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCcgcg > 5:71565/1‑148 (MQ=255) tgtgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGGTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAg < 1:51743/149‑1 (MQ=255) tgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCGAAggg < 3:125774/149‑1 (MQ=39) gTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTgc < 6:71565/148‑1 (MQ=255) aaTGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGGGGGCAAGCCCGCGCAGGGTTgcaacg > 6:175287/1‑149 (MQ=255) tGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCCGCCCTTGGCGGGGAAGCCCGCGGAGGGTTgcaacgcc > 8:83444/1‑148 (MQ=255) gCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTTCAGCCCATAGCGGGGAAGCGCGGCCACGGGTGGAACGC‑AAcc > 6:305190/1‑149 (MQ=255) gCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGATGAGCGTACCGGGGTTGCGTTTCACCCCATATCGTGGAATCGCGCGTAGGGTTggaacga‑aaac > 3:277282/1‑147 (MQ=255) aaTGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTTCAGCCCATAGCGTTCAAGCCCGCGAAGGGTTGCAACGC‑AAccc > 6:69553/1‑148 (MQ=255) ggtggtGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGGGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTCCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGC‑AACCCCaaaa < 5:175287/149‑1 (MQ=255) tggtgCGCCAGGGGCGGCATGCCCAGGATTATGAATGCGGGGGGGCGTTTGCCATGGTGTTGCGCCTGCTTTTGGGTTGAGCGTACGGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCTAAGGGTTGAAACGC‑AACCCCaaaaaa < 6:133354/149‑1 (MQ=255) gtgCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGGGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGC‑AACCCCAAAAAACt < 7:83444/149‑1 (MQ=255) tgCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAgg > 4:31208/1‑124 (MQ=255) tgCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAgg < 3:31208/124‑1 (MQ=255) cgcCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGGTTCAGCCCATAGCGGGGAAGCCCGCGCAGGGTTTGCACCC‑ACCCCCcaaaaaagaag > 8:24093/1‑144 (MQ=255) ccAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCATCGCGTGGAAGCCCGCGAAGGGTTGGAACGC‑CACCCCCAAAAACTCAGTg > 1:53131/1‑149 (MQ=255) cAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGGGTTGCAGCCCATCACCGGGAAGCCCCGGAACGGTTGGAACGCAAACCCCaaaaaaggaatg > 6:351977/1‑142 (MQ=255) ccAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCTAAGGGTTGCAACGC‑AACCCCAAAAAACTCAGTGCAtttt < 5:125120/140‑1 (MQ=255) gctggcgggcgTTTGCCAAGGTTTTGCTCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGGGTGCAAGCCCGCTAAGGGTTGCAACGC‑AACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAcgccc < 4:277282/146‑1 (MQ=255) gcgggcgTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGC‑AACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCa < 4:222168/149‑1 (MQ=255) gTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGC‑AACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGAt < 5:183072/130‑1 (MQ=255) gtgtTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGC‑AACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGgtg < 5:69553/149‑1 (MQ=255) ttttGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGC‑AACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAc < 5:359416/112‑1 (MQ=255) gTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGC‑AACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGa < 5:351977/149‑1 (MQ=255) gggTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGC‑AACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCGGGAcagcag < 5:305190/149‑1 (MQ=255) cAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGC‑AACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTc < 6:322915/108‑1 (MQ=255) cAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGC‑AACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTc > 5:322915/1‑108 (MQ=255) aGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGC‑AACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTc > 7:198842/1‑149 (MQ=255) aGCCCTTCGCGGGCAAGCCCGCGAAGGGTTGCAACGC‑AACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTc < 7:24093/149‑1 (MQ=255) cccTTCGCGGGCTTGCACGCGAAGGGTTGCAACGC‑AACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtctc > 6:114918/1‑141 (MQ=255) cccTTCGCGGGCTTGCACGCGAAGGGTTGCAACGC‑AACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtctc < 5:114918/141‑1 (MQ=255) cTTCGCGGGCTTGCACGCGAAGGGCTGCAACGC‑AACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAg > 6:229650/1‑149 (MQ=39) ttCGCGGGCTTGCACGCGAAGGGCTGCAACGC‑AACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAca > 5:234351/1‑128 (MQ=38) ttCGCGGGCTTGCACGCGAAGGGCTGCAACGC‑AACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAca < 6:234351/128‑1 (MQ=38) cAAGCCCGCGAAGGGTTGCAACGC‑AACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCgg > 7:333781/1‑145 (MQ=255) cAAGCCCGCGAAGGGTTGCAACGC‑AACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCgg < 8:333781/145‑1 (MQ=255) | GGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGC‑AACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGG > NC_002947/5740324‑5740613 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |