Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	474,161	A→T	6.2%	L275Q (CTG→CAG)	dnaG ←	DNA primase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	474,161	0	A	T	6.2%	195.4 / 3.0	64	L275Q (CTG→CAG)	dnaG	DNA primase
Reads supporting (aligned to +/- strand):  ref base A (28/32);  new base T (2/2);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01

GTGCGCGCCAGGCGGCCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGC  >  NC_002947/474015‑474308                                                                                                                                                   |                                                                                                                                                     gTGCGCGCCAGGCGGCCGTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGGGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCCTTGCGCAGGCCTTGCTGGGCCAgg                                                                                                                                                    <  2:164916/149‑1 (MQ=255)     gcgcCAGGCGGCCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCaa                                                                                                                                                <  2:168120/149‑1 (MQ=255)      cgcCAGGCGGCCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAAt                                                                                                                                               <  6:329477/149‑1 (MQ=255)       gcCAGGCGGCCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATg                                                                                                                                              >  7:70177/1‑149 (MQ=255)       gcCAGGCGGCCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCCCTGGCCCAGCAAGGCCTGCGCAATg                                                                                                                                              >  7:139093/1‑149 (MQ=255)       gcCAGGCGGCCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCCCTGGCCCAGCAAGGCCTGCGCAATg                                                                                                                                              <  4:154800/149‑1 (MQ=255)         cAGGCGGCCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGAc                                                                                                                                            >  6:263249/1‑149 (MQ=255)         cAGGCGGCCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCCCTGGCCCAGCAAGGCCTGCGCAATgcc                                                                                                                                            <  8:139093/149‑3 (MQ=38)              ggcCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCa                                                                                                                                       >  4:108536/1‑149 (MQ=255)                ccTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATg                                                                                                                                     <  6:60136/149‑1 (MQ=255)                  ttGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATg                                                                                                                                     >  4:192580/1‑147 (MQ=255)                      gcCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGccc                                                                                                                               >  4:122304/1‑149 (MQ=255)                        ccGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTc                                                                                                                             >  1:143174/1‑149 (MQ=255)                         cGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTcg                                                                                                                            >  8:242413/1‑149 (MQ=255)                         cGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTcg                                                                                                                            <  2:356663/149‑1 (MQ=255)                          ggCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTcga                                                                                                                           >  8:211876/1‑149 (MQ=255)                           gCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCCGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTcgac                                                                                                                          >  1:113035/1‑149 (MQ=255)                                gTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTcgacgacga                                                                                                                     <  3:192580/149‑1 (MQ=255)                                 tCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCTGTGACAAGGGTGGCCACGGCATTGCGCAGTCCTTGCTGGGCCAGGGAAATTACGTCCATGTAGCCCTCGACGACgat                                                                                                                    >  8:135419/1‑149 (MQ=255)                                   gCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACgatg                                                                                                                   <  5:124698/148‑1 (MQ=255)                                          aaaCAGAACAGCACGCTGGGCACCACGCGGAACAGGAGCTTGAGGTGCTCTTCGCTGGTGGAGGTTCCAAGGGTGTCCACGGAATTGCGCAGTCCTTGCTGGGCAAGGGCAATGAAGTCCATGTAGCCCTCGACGACGATGATC‑tcgtc                                                                                                          >  8:298197/1‑149 (MQ=255)                                             cagaacagCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGA‑GATCATCGTCGTCGAg                                                                                                       <  2:66986/149‑1 (MQ=255)                                              agaacagCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAgg                                                                                                      <  3:127471/149‑1 (MQ=255)                                              agaacagCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAgg                                                                                                      >  8:224638/1‑149 (MQ=255)                                                   agCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCCATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCg                                                                                                 >  6:266616/1‑149 (MQ=255)                                                   agCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCg                                                                                                 >  8:36071/1‑149 (MQ=255)                                                    gCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCgg                                                                                                <  3:191675/149‑1 (MQ=255)                                                            ggCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGc                                                                                          <  2:113035/147‑1 (MQ=255)                                                                ccacGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTCGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTgcgc                                                                                    <  4:275483/149‑1 (MQ=255)                                                                 cacGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTgcgcg                                                                                   >  6:186194/1‑149 (MQ=255)                                                                   cgcgGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGcc                                                                                 <  7:211876/149‑1 (MQ=255)                                                                     cgGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCt                                                                                <  5:145021/148‑1 (MQ=255)                                                                     cgGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCt                                                                                >  6:145021/1‑148 (MQ=255)                                                                        aaCAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTa                                                                            <  5:186194/149‑1 (MQ=255)                                                                               gcTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCg                                                                     >  8:21478/1‑149 (MQ=255)                                                                                   gAGGTGCTCTTCGCTTGTGGCGGTGCCAAGGGTGTCCAAGGCATTGCGCAGGCCTTGCTGGGCCATGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTAc                                                                  <  7:135419/148‑1 (MQ=255)                                                                                   gAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTa                                                                   <  5:263249/147‑1 (MQ=255)                                                                                     ggTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACTGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTcga                                                                                                                           <  6:309850/90‑1 (MQ=255)                                                                                     ggTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTcga                                                                                                                           >  5:309850/1‑90 (MQ=255)                                                                                         ctctTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTa                                                                            <  6:16021/132‑1 (MQ=255)                                                                                         ctctTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTa                                                                            >  5:16021/1‑132 (MQ=255)                                                                                         ctctTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCt                                                           <  7:21478/149‑1 (MQ=255)                                                                                            ttCGCTGGTGGCGGTGCCAAGGGTGGCCTCGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGcc                                                        >  1:204746/1‑149 (MQ=255)                                                                                               gCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCt                                                     >  3:80234/1‑149 (MQ=255)                                                                                                         gTGCCAAGGGTGGCCACGGCATTGAGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCTTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCGCCTGACCCTTGTGGATAAc                                           >  3:186222/1‑149 (MQ=255)                                                                                                           gCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCg                                         >  3:245268/1‑149 (MQ=255)                                                                                                             cAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCggg                                       <  8:70177/149‑1 (MQ=255)                                                                                                             cAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCggg                                       >  8:65968/1‑149 (MQ=255)                                                                                                             cAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCggg                                       <  2:204746/149‑1 (MQ=255)                                                                                                                   tgtccACGGCATTGCGCAGGCCTTGCTTGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTca                                 <  4:186222/146‑2 (MQ=255)                                                                                                                     gCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCgg                               <  7:36071/149‑1 (MQ=255)                                                                                                                     gCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCgg                               <  5:266616/149‑1 (MQ=255)                                                                                                                           tcATTGCCCTGGCCCAGCAAGGCCTGAGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGt                          >  6:305520/2‑148 (MQ=255)                                                                                                                           gCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGtt                         <  7:242413/149‑1 (MQ=255)                                                                                                                            cATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTc                        <  4:245268/149‑1 (MQ=255)                                                                                                                                 cgcAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGt                    <  3:108536/148‑1 (MQ=255)                                                                                                                                 cgcAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTa                   >  2:71326/1‑149 (MQ=255)                                                                                                                                      gCCTTGCTGGGCCAGGGCAATGACGTCCATTTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAAcc                                          >  2:331464/1‑121 (MQ=255)                                                                                                                                      gCCTTGCTGGGCCAGGGCAATGACGTCCATTTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAAcc                                          <  1:331464/121‑1 (MQ=255)                                                                                                                                         ttGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCg                                         >  7:167015/1‑119 (MQ=255)                                                                                                                                         ttGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCg                                         <  8:167015/119‑1 (MQ=255)                                                                                                                                                gCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTtgtg                                                 <  3:229900/104‑1 (MQ=255)                                                                                                                                                gCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCCAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTtgtg                                                 >  4:229900/1‑104 (MQ=255)                                                                                                                                                  cAGGTCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGc  <  7:105372/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     GTGCGCGCCAGGCGGCCTTGCGCCCGGCCTGGTCGCCGTCAAAACAGAACAGCACGCTGGGCACCACGCGGAACAGGCGCTTGAGGTGCTCTTCGCTGGTGGCGGTGCCAAGGGTGGCCACGGCATTGCGCAGGCCTTGCTGGGCCAGGGCAATGACGTCCATGTAGCCCTCGACGACGATGATC‑TCGTCGAGGTTGCGGTTGTGCTTGCGCGCCTCGTACAGCCCGTACAGCTCCTGCCCCTTGTGGAAAACCGGGGTTTCCGGGGAGTTCAGGTACTTGGGCTTGTCGTCGC  >  NC_002947/474015‑474308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑