Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,749,603 A→G 6.9% V126A (GTG→GCG)  typA ← ribosome associated GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,749,6030AG 6.9% 166.6 / 2.0 58V126A (GTG→GCG) typAribosome associated GTPase
Reads supporting (aligned to +/- strand):  ref base A (29/25);  new base G (2/2);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

CGGCAATGCCGTTCAGGGCCGAGGCGTAGACCACTTTGAAGTCCAGCTGTTCGTCGGTGGCACCGAGGTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACCAGCAGCAGCACGGAGTCGACCATCGACATTACACGCTCAACCTCGCCACCGAAGTCGGCGTGG  >  NC_002947/5749463‑5749749
                                                                                                                                              |                                                                                                                                                  
cGGCAATGCCGTTCAGGGCCGAGGCGTAGACCACTTTGAAGTCCAGCTGTTCGTCGGTGGCACCGAGGTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGAtt                                                                                                                                            >  2:60845/1‑149 (MQ=255)
cGGCAATGCCGTTCAGGGCCGAGGCGTAGACCACTTTGAAGTCCAGCTGTTCGTCGGTGGCACCGAGGTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGAtt                                                                                                                                            >  8:216841/1‑149 (MQ=255)
     aTGCCGTTCAGGGCCGAGGCGTAGACCACTTTGAAGTCCAGCTGTTCGTCGGTGGCACCGAGGTTGTCTAAGACAGGTCGAAGATCTCGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCGCGACGATTGGc                                                                                                                                         >  1:292547/1‑149 (MQ=255)
     aTGCCGTTCAGGGCCGAGGCGTAGACCACTTTGAAGTCCAGCTGTTCGTCGGTGGCACCGAGGTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCGCGACGATTGGCt                                                                                                                                        >  3:215687/1‑148 (MQ=255)
                  ccGAGGCGTAGACCACTTTGAAGTCCAGCTGTTCGTCGGTGGCACCGAGGTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTc                                                                                                                           >  4:222051/1‑148 (MQ=255)
                  ccGAGGCGTAGACCACTATGAAGTCCAGCTGTTCGTCGGTGGCACCGAGGTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTc                                                                                                                           >  4:332446/1‑148 (MQ=255)
                     aGGCGTAGACCACTTTGAAGTCCAGCTGTTCGTCGGTGGCACCGAGGTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGaaa                                                                                                                       <  3:222051/149‑1 (MQ=255)
                        cGTAGACCACTTTGAAGTCCAGCTGTTCGTCGGTGGCACCGAGGTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGcc                                                                                                                    <  2:205361/149‑1 (MQ=255)
                              ccACTTTGAAGTCCAGCTGTTCGTCGGTGGCACCGAGGTTGTCGA‑‑ACAGGTGGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCTACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTg                                                                                                              <  8:45621/149‑1 (MQ=255)
                              ccACTTTGAAGTCCAGCTGTTCGTCGGTGGCACCGAGGTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCGGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTg                                                                                                              >  2:119128/1‑149 (MQ=255)
                              ccACTTTGAAGTCCAGCTGTTCGTCGGTGGCACCGAGGTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTg                                                                                                              <  8:39919/149‑1 (MQ=255)
                                         tCCAGCTGTTCGTCGGTGGCACCGAGGTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCg                                                                                                   >  8:180347/1‑149 (MQ=255)
                                              cTGTTCGTCGGTGGCACCGAGGTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGGCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGttt                                                                                              <  8:300103/149‑1 (MQ=255)
                                                gTTCGTCGGTGGCACCGAGGTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCATGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGc                                                                                            >  7:45226/1‑149 (MQ=255)
                                                 ttCGTCGGTGGCACCGAGGTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTg                                                                                             <  7:180347/147‑1 (MQ=255)
                                                    gtcgGTGGCACCGAGGTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCACCCTTGTTGATCACGACGATTGGCTTCAGGCGGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCa                                                                                        >  5:309185/1‑149 (MQ=255)
                                                    gtcgGTGGCACCGAGGTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCa                                                                                        >  3:203585/1‑149 (MQ=255)
                                                     tcgGTGGCACCGAGGTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCAt                                                                                       >  2:274940/1‑149 (MQ=255)
                                                         tGGCACCGAGGTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCTAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCGCGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCAGCggc                                                                                   <  4:215687/149‑2 (MQ=255)
                                                         tGGCACCGAGGTTGGCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCGCGACGATTGGCTTCGGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCAGCggc                                                                                   <  2:292547/149‑2 (MQ=255)
                                                            cACCGAGGTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCg                                                                                >  3:311520/1‑149 (MQ=255)
                                                                 aGGTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTg                                                                           <  3:332446/149‑1 (MQ=255)
                                                                  ggTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCGGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTgg                                                                          <  1:119128/149‑1 (MQ=255)
                                                                   gTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTggg                                                                         >  2:330438/1‑149 (MQ=255)
                                                                       tCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGt                                                                      <  7:216841/148‑1 (MQ=255)
                                                                         gA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTg                                                                                                              >  2:345587/1‑106 (MQ=255)
                                                                         gA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTg                                                                                                              <  1:345587/106‑1 (MQ=255)
                                                                             aCAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGaaa                                                                                                                       <  8:23295/95‑1 (MQ=255)
                                                                             aCAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGaaa                                                                                                                       >  7:23295/1‑95 (MQ=255)
                                                                                 gTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGAtt                                                                                                                                            <  2:266960/70‑1 (MQ=255)
                                                                                 gTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGAtt                                                                                                                                            >  1:266960/1‑70 (MQ=255)
                                                                                 gTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGACCTGGGCGTCGACcag                                                               >  3:104858/1‑147 (MQ=255)
                                                                                  tCGAAGATCTGGTCGAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACcagcag                                                            >  7:306304/1‑149 (MQ=255)
                                                                                  tCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACcagcag                                                            >  8:121995/1‑149 (MQ=255)
                                                                                  tCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACcagcag                                                            >  5:39872/1‑149 (MQ=255)
                                                                                   cGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACcagcagc                                                           <  1:330438/149‑1 (MQ=255)
                                                                                       gATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGTTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACCAgcagcagca                                                       >  3:248354/1‑149 (MQ=255)
                                                                                             gTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACCAGCAGCAGCACGGAGt                                                 <  1:60845/149‑1 (MQ=255)
                                                                                             gTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACCAGCAGCAGCACGGAGt                                                 >  1:183416/1‑149 (MQ=255)
                                                                                                 aGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACCAGCAGCAGCACGGAGTCGAc                                             <  8:82338/149‑1 (MQ=255)
                                                                                                  gAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTCTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCCGGCCGTCCTGGTCGTCGACCAGCAGCAGCACGGAGTCGAc                                             >  8:187285/1‑148 (MQ=255)
                                                                                                      ccAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACcagc                                                              <  8:322923/127‑1 (MQ=255)
                                                                                                      ccAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACcagc                                                              >  7:322923/1‑127 (MQ=255)
                                                                                                        aGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACCAGCAGCAGCACGGAGTCGACCATCGa                                       >  3:277849/1‑148 (MQ=255)
                                                                                                         gTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACCAGCAGCAGCACGGAGTCGACCATCGACa                                     <  4:311520/149‑1 (MQ=255)
                                                                                                          tCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACCAGCAGCAGCACGGAGTCGACCATCGACAt                                    <  8:306304/149‑1 (MQ=255)
                                                                                                            aGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACCAGCAGCAGCACGGAGTCGACCATCGACAtt                                   <  4:277849/148‑1 (MQ=255)
                                                                                                               aCGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCATGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACCAGCAGCAGCAAGGAGTCGACCATCGACATTacac                               >  1:70515/1‑149 (MQ=255)
                                                                                                                 gTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACCAGCAGCAGCACGGAGTCGACCATCGACATTACACGc                             <  4:104858/149‑1 (MQ=255)
                                                                                                                   gcgcCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACCAGCAGCAGCACGGAGt                                                 >  3:229679/1‑127 (MQ=255)
                                                                                                                   gcgcCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACCAGCAGCAGCACGGAGt                                                 <  4:229679/127‑1 (MQ=255)
                                                                                                                    cgcCCGGACGGGCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCGTCGAAAGCCTTCTTGGGCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACCAGCATCAGCACGGAGTCGACCATCGACATTACACGCTc                           <  6:309185/148‑1 (MQ=255)
                                                                                                                               acAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTGCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACCAGCAGCAGCACGGAGTCGACCATCGACAt                                    <  8:147931/127‑1 (MQ=255)
                                                                                                                                 aaCCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACCAGCAGCAGCACGGAGTCGACCATCGACATTACACGCTCAACCTCGCCACCg              >  1:170276/1‑148 (MQ=255)
                                                                                                                                    cTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGTGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACCAGCAGCTGCACGGAGTCGACCATCGACATTACACGCTCAACGTCGCCACCGAAGt          <  7:187285/149‑1 (MQ=255)
                                                                                                                                          gATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACCAGCAGCAGCACGGAGTCGACCATCGACATTac                                 <  8:152643/120‑1 (MQ=255)
                                                                                                                                            tCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACCAGCAGCAGCACGGAGTCGACCATCGACATTACACGCTCAACCTCGCCACCGAAGTCGGCGTgg  >  4:84720/1‑149 (MQ=255)
                                                                                                                                            tCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACCAGCAGCAGCACGGAGTCGACCATCGACATTACACGCTCAACCTCGCCACCGAAGTCGGCGTgg  >  2:41634/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
CGGCAATGCCGTTCAGGGCCGAGGCGTAGACCACTTTGAAGTCCAGCTGTTCGTCGGTGGCACCGAGGTTGTCGA‑‑ACAGGTCGAAGATCTGGTCCAGAACCCAGTCAGGACGTGCGCCCGGACGGTCAACCTTGTTGATCACGACGATTGGCTTCAGGCCGGCTTCGAAAGCCTTCTTGGTCACGAAGCGGGTTTGCGGCATCGGGCCGTCCTGGGCGTCGACCAGCAGCAGCACGGAGTCGACCATCGACATTACACGCTCAACCTCGCCACCGAAGTCGGCGTGG  >  NC_002947/5749463‑5749749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑