Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,754,243 C→G 62.3% P147A (CCC→GCC)  glnL → two‑component system sensor histidine kinase/phosphatase GlnL/GlnG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,754,2430CG62.3% 24.9 / 45.4 43P147A (CCC→GCC) glnLtwo‑component system sensor histidine kinase/phosphatase GlnL/GlnG
Reads supporting (aligned to +/- strand):  ref base C (9/7);  new base G (14/12);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.06e-01

GCATCAAGGCCAGACCCTGCTGCTGCTCGAGGTGCACCCGCGTGACCGGCTGCTGCGCATCACCAAGGAAGAGGCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACCCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAGGGCTGCGCGACTACACCAATGTGATCATCGAGGAGGCCGACCGCTTGCGTAACCTGGTCGACCGCATGCTCGGCTCGAACAAGCTG  >  NC_002947/5754104‑5754386
                                                                                                                                           |                                                                                                                                               
gCATCAAGGCCAGACCCTGCTGCTGCTCGAGGTGCACCCGCGTGACCGGCTGCTGCGCATCACCAAGGAAGAGGCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACGCCCTTggcg                                                                                                                                        >  5:267104/1‑149 (MQ=255)
gCATCAAGGCCAGACCCTGCTGCTGCTCGAGGTGCACCCGCGTGACCGGCTGCTGCGCATCACCAAGGAAGAGGCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACGCCCTTggcg                                                                                                                                        >  2:215787/1‑149 (MQ=255)
 cATCAAGGCCAGACCCTGCTGCTGCTCGACGTGCACCCGCGTGACCGGCTGCTGCGCATCACCAAGGAAGAGGCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACCCCCTTggcg                                                                                                                                        <  7:92188/148‑1 (MQ=255)
        gCCAGACCCTGCTGCTGCTCAAGGTGCACCCGCGTGACCGGCTGCTGCGCATCACCAAGGAAGAGGCCCAACTGACCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACGCCCTTGGCGGCATAcgc                                                                                                                                >  4:8358/1‑149 (MQ=255)
         ccAGACCCTGCTGCTGCTCGAGGTGCACCCGCGTGACCGGCTGCTGCGCATCACCAAGGAAGAGGCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACGCCCTTGGCGGCATCcgcg                                                                                                                               <  1:215787/149‑1 (MQ=255)
          cAGACCCTGCTGCTGCTCGAGGTGCACCCGCGTGACCGGCTGCTGCGCATCACCAAGGAAGAGGCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACGCCCTTGGCGGCATCcgcg                                                                                                                               >  3:14751/1‑148 (MQ=255)
           aGACCCTGCTGCTGCTCGAGGTGCACCCGCGGGACCGGCTGCTGCGCATCACCAAGGAAGAGGCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACGCCCTTGGCGGCATCCGCGGa                                                                                                                             <  6:163223/149‑1 (MQ=255)
                 tgctgctgcTCGAGGTGCACCCGCGTGACCGGCTGCTGCGCATCACCAAGGAAGAGGCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACCCCCTTGGCGGCATCCGCGGAGCGGcc                                                                                                                       >  6:202968/1‑149 (MQ=255)
                  gctgctgctCGAGGTGCACCCGCGTGACCGGCTGCTGAGCATCACCAAGGAAGAGGACCAACTGAGCAAGAAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAATAACGCCCATGGCGGCATCCGCGGAGCGGcc                                                                                                                       >  8:192037/1‑148 (MQ=255)
                   ctgctgctCGAGGTGCACCCGCGTGACCGGCTGCTGCGCATCACCAAGGAAGAGGCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACCCCCTTGGCGGCATCCGCGGAGCGGCCCa                                                                                                                     <  3:344974/149‑1 (MQ=255)
                   ctgctgctCGAGGTGCACCCGCGTGACCGGATGCTGCGCATCACAAAGGAAGAGGCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACGCCATTGGCGGCATCCGCGGAGCGGca                                                                                                                       >  5:320984/1‑146 (MQ=255)
                            gAGGTGCACCCGCGTGACCGGCTGCTGCGCATCACCAAGGAAGAGGCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACGCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGc                                                                                                            <  5:127591/149‑1 (MQ=255)
                                      cgcgAGACCGGCTGCTGCGCATCACCAAGGAAGAGCCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGACCGCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACAGCCCCGCGAACTg                                                                                                  >  5:55546/1‑149 (MQ=255)
                                       gcgTGACCGGCTGCTGCGCATCACCAAGGAAGAGGCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCACGAACCCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGc                                                                                                 <  5:202968/149‑1 (MQ=255)
                                       gcgTGACCGGCTGCTGCGCATCACCAAGGAAGAGGCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACGCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGc                                                                                                 <  6:267104/149‑1 (MQ=255)
                                                gctgctGCGCATCACCAAGGAAGAGGCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACGCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAg                                                                                        <  4:14751/149‑1 (MQ=255)
                                                    ctgcGCATCACCAAGGAAGAGGCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACGCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAGGGCt                                                                                    <  4:86458/149‑1 (MQ=255)
                                                               cAAGGAAGAGGCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACCCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAgg                                                                                       >  4:76572/1‑135 (MQ=255)
                                                               cAAGGAAGAGGCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACCCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAgg                                                                                       <  3:76572/135‑1 (MQ=255)
                                                               cAAGGAAGAGGCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACCCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAGGGCTGCGCGACTaca                                                                         >  8:212557/1‑149 (MQ=255)
                                                                aaGGAAGAGGCCCAACTGAGCAATCAGGAAACCTCCAAGATGCTGGTGCGCGGCCTTGCCCACGAAATCAAGAACTCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGATGAAGGGCTGCGCGACTacac                                                                        <  7:192037/149‑1 (MQ=255)
                                                                    aaGAGGCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACGCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAGGGCTGCGCGACTACACCAAt                                                                    <  3:8358/149‑1 (MQ=255)
                                                                        ggCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACGCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAGGGCTGCGCGACTACACCAATGTGa                                                                >  3:326031/1‑149 (MQ=255)
                                                                         gCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACCCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTAATGGCCCGCGAACTGCCAGAGGAAGGGCTGCGCGACTACACCAATGTCat                                                               >  5:74010/1‑149 (MQ=255)
                                                                           ccAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACCCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAg                                                                                        <  1:359692/122‑1 (MQ=255)
                                                                           ccAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACCCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAg                                                                                        >  2:359692/1‑122 (MQ=255)
                                                                               cTGAGCAAGCAGGAAACCACCAAGATGCTGGTGAGCGGCCTGGCCCACGAAATCAAAAACGCCCTTGGCGGCATCCGCGGAGCGGCCAAGCTACTGGACCGCGAAATCCCAAAGGAACGGCAGCGCGACTAAACCAAAGTGAACATCga                                                         >  7:226654/1‑149 (MQ=255)
                                                                                      agcaGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACGCCCTTGGCGGCATCCGCGGAGCGGCCAAGCTACTGGCCCGCGAACTGCCAGAGGAAGGGCTGCGCGACTACACCAATGTGATCATCGAGGAGGcc                                                  >  1:108517/1‑149 (MQ=255)
                                                                                       gcaGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACGCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAGGGCTGCGCGACTACACCAATGTGATCATCGAGGAGGcc                                                  >  4:159056/1‑148 (MQ=255)
                                                                                       gcaGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACCCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAGGGCTGCGCGACTACACCAATGTGATCATCGAGGAGGCCg                                                 >  1:325756/1‑149 (MQ=255)
                                                                                           gAACCCCCCATGTTGCTGGTGCGCGGCCTGGCCCACGAAATCAAGTCCGCCCTTGGCGGCATCCGCGGAGCTGCCCTGCTACTGTCCCGCGAACTGCCAGTGGAAGGGCTGCGCGATTACACCATTGTGATCATCGAGGAGGCCGACCg                                             <  8:226654/149‑1 (MQ=255)
                                                                                                caccaAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACGCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAGGGCTGCGCGACTACACCAATGTGATCATCGAGGATGCCGACCGCTTGc                                        <  3:159056/149‑1 (MQ=255)
                                                                                                caccaAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACCCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAGGGCTGCGCGACTACACCAATGTGATCATCGAGGAGGCCGACCGCTTGc                                        >  6:202934/1‑149 (MQ=255)
                                                                                                caccaAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACCCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAGGGCTGCGCGACTACACCAATGTGATCATCGAGGAGGCCGACCGCTTGc                                        <  5:202934/149‑1 (MQ=255)
                                                                                                   caAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACGCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAGGGCTGCGCGACTACACCAATGTGATCATCGAGGAGGCCGACCGCTTGCGt                                      >  3:217690/1‑148 (MQ=255)
                                                                                                             gTGCGCGGCCTGGCCCACGAAATCAAGAACGCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAGGGCTGCGCGACTACACCAATGTGATCATCGAGGAGGCCGACCGCTTGCGTAACCTGGTc                             <  4:326031/147‑1 (MQ=255)
                                                                                                                 gcgGCCTGGCCCACGAAATCAAGAACGCCCTTGGCGGCATCcgcg                                                                                                                               >  6:17997/1‑45 (MQ=255)
                                                                                                                 gcgGCCTGGCCCACGAAATCAAGAACGCCCTTGGCGGCATCcgcg                                                                                                                               <  5:17997/45‑1 (MQ=255)
                                                                                                                        ggCCCACGAAATCAAGAACCCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAGGGCTGCGCGACTACACCAATGTGATCATCGAGGAGGCCGACCGCTTGCGTAACCTGGTCGACCGCATgctcg                >  5:345528/1‑149 (MQ=255)
                                                                                                                             aCGAAATCAAGAACGCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAGGGCTGCGCGACTACACCAATGTGATCATCGAGGAGGCCGACCGCTTGCGTAACCTGGTCGACCGCATgctcggctcg           <  1:88307/149‑1 (MQ=255)
                                                                                                                                 aaTCAAGAACGCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAGGGCTGCGCGACTACACCAATGTGATCATCGAGGAGGCCGACCGCTTGCGTAACCTGGTCGACCGCATGCTCGGCTCGAAc        >  7:334667/1‑148 (MQ=255)
                                                                                                                                     aaGAACCCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAGGGCTGCGCGACTACACCAATGTGATCATCGAGGAGGCCGACCGCTTGCGTAACCTGGTCGACCGCATGCTCGGCTCGAACAAGCt   >  2:5425/1‑149 (MQ=255)
                                                                                                                                      aGAACCCCCTTGGCGGCAGCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAGGGCTGCGCGACTACACCAATGTGATCATCGAGGAGGCCGACCGCTTGCGTAACCTGGTCGACCGCATGCTCGGCTCGAACAAGCTg  <  4:210282/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                               
GCATCAAGGCCAGACCCTGCTGCTGCTCGAGGTGCACCCGCGTGACCGGCTGCTGCGCATCACCAAGGAAGAGGCCCAACTGAGCAAGCAGGAAACCACCAAGATGCTGGTGCGCGGCCTGGCCCACGAAATCAAGAACCCCCTTGGCGGCATCCGCGGAGCGGCCCAGCTACTGGCCCGCGAACTGCCAGAGGAAGGGCTGCGCGACTACACCAATGTGATCATCGAGGAGGCCGACCGCTTGCGTAACCTGGTCGACCGCATGCTCGGCTCGAACAAGCTG  >  NC_002947/5754104‑5754386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑