Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,813,220	C→G	6.8%	I47M (ATC→ATG)	argS →	arginine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,813,220	0	C	G	6.8%	186.1 / 3.7	59	I47M (ATC→ATG)	argS	arginine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (27/28);  new base G (2/2);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.73e-01

AAAGACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCT  >  NC_002947/5813083‑5813366                                                                                                                                          |                                                                                                                                                    aaaGACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGGGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGc                                                                                                                                           <  7:187696/148‑1 (MQ=255)  aaGACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTg                                                                                                                                         <  2:238517/149‑1 (MQ=255)  aaGACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTg                                                                                                                                         <  8:243806/149‑1 (MQ=255)       acCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCATCATGGCGATGTTGCTGGCGaa                                                                                                                                    <  1:9143/149‑1 (MQ=255)       acCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGc                                                                                                                                                   <  2:5125/134‑1 (MQ=255)       acCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGc                                                                                                                                                   >  1:5125/1‑134 (MQ=255)          aTTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGAGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAgcc                                                                                                                                 >  8:38208/1‑149 (MQ=255)          aTTCGCCAGCTGATCCAGCAACCCCTCACCCAACTCGTCACCGACGGTCTGCTGCCTGAAGGGCTGACGCCCGCGAACCAGGTGGAAAACGCCCGGGACAACACCCACGCCGAAATAGCCAGCAACATCGCCATGAAGCTGGCCAAgcc                                                                                                                                 >  8:183472/1‑149 (MQ=255)                 aGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATCATGCTGGCCAAGccggccgg                                                                                                                           >  6:32747/1‑148 (MQ=255)                      aTCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGa                                                                                                                      <  5:341830/148‑1 (MQ=255)                         cAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑Gcc                                                                                                                 >  3:194806/1‑149 (MQ=255)                         cAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCAACTTCGCCAGCATCATGGCGATGTTGCTgg                                                                                                                                        >  5:85864/1‑127 (MQ=255)                         cAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCAACTTCGCCAGCATCATGGCGATGTTGCTgg                                                                                                                                        <  6:85864/127‑1 (MQ=255)                             aaGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCcgcgc                                                                                                             <  7:226816/149‑1 (MQ=255)                               gCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGAGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGa                                                                                                           <  7:38208/149‑1 (MQ=255)                                 ccTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGGTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGAcc                                                                                                         >  2:240053/1‑149 (MQ=255)                                  cTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCt                                                                                                        >  8:78424/1‑149 (MQ=255)                                     aCCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGc                                                                                                     <  3:337303/149‑1 (MQ=255)                                     aCCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGc                                                                                                     >  1:308631/1‑149 (MQ=255)                                       ccAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGcc                                                                                                    >  6:241879/1‑148 (MQ=255)                                       ccAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGcc                                                                                                    >  4:69932/1‑148 (MQ=255)                                                 aCCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGccggccgg                                                                                                                           <  3:157280/116‑1 (MQ=255)                                                 aCCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGccggccgg                                                                                                                           >  4:157280/1‑116 (MQ=255)                                                 aCCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGAt                                                                                         <  1:312596/149‑1 (MQ=255)                                                  ccGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAACGCCGCGCGACCTCGCCGAAAAACTGAt                                                                                         <  1:198635/149‑1 (MQ=255)                                                  ccGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATc                                                                                        <  5:32747/149‑1 (MQ=255)                                                                  tGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGc                                                                          >  8:51916/1‑147 (MQ=255)                                                                      gggCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAgcgc                                                                    <  7:280005/149‑1 (MQ=255)                                                                      gggCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAgcgc                                                                    <  4:132857/149‑1 (MQ=255)                                                                       ggCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGcc                                                                   <  7:78424/149‑1 (MQ=255)                                                                        gCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGcc                                                                   >  8:19409/1‑148 (MQ=255)                                                                        gCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCACCGcc                                                                   >  7:63282/1‑148 (MQ=255)                                                                            tCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGAc                                                                <  4:194806/147‑1 (MQ=255)                                                                              gCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATc                                                             <  7:51916/148‑1 (MQ=255)                                                                               ccGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAg                                                           >  6:122058/1‑149 (MQ=255)                                                                                         aGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTc                                                    >  7:3986/1‑146 (MQ=255)                                                                                             ggAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTg                                             >  2:57257/1‑149 (MQ=255)                                                                                                aaaCGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAg                                          >  1:72801/1‑149 (MQ=255)                                                                                                    gCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGccc                                      <  1:57257/149‑1 (MQ=255)                                                                                                        gggACAAGACCCACGGCGACTTCGCCAGCAACATCGcc                                                                                                                                                  >  8:154663/1‑38 (MQ=255)                                                                                                        gggACAAGACCCACGGCGACTTCGCCAGCAACATCGcc                                                                                                                                                  <  7:154663/38‑1 (MQ=255)                                                                                                        gggACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGc                                  <  7:19409/149‑1 (MQ=255)                                                                                                             aaGACCCACGGCGACTACGCCAGCAACATCGCCATGAGGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTCATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCt                             <  2:296160/149‑1 (MQ=255)                                                                                                               gACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTc                            >  3:270405/1‑148 (MQ=255)                                                                                                                 cccACGGCGACTTCGCCAGCAACATCGCCATGATGGTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAAc                         <  1:240053/149‑1 (MQ=255)                                                                                                                  ccACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAAct                        >  8:39841/1‑149 (MQ=255)                                                                                                                  ccACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAAct                        >  5:189818/1‑149 (MQ=255)                                                                                                                    aCGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAActtc                      >  2:177700/1‑149 (MQ=255)                                                                                                                     cGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCg                                    <  7:210637/134‑1 (MQ=255)                                                                                                                     cGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCg                                    >  8:210637/1‑134 (MQ=255)                                                                                                                     cGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAActtct                     >  4:155569/1‑149 (MQ=255)                                                                                                                       gCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACttcttc                   <  2:308631/149‑1 (MQ=255)                                                                                                                        cGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTcc                  <  1:177700/149‑1 (MQ=255)                                                                                                                        ataCTTCGCCAGCTACATCGCCATGATGCTGGCCAAGCCTGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCACTTACGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTcc                  <  7:183472/147‑1 (MQ=255)                                                                                                                          gcTTGGCCAGCATCATGGCGATGTTGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACttcttc                   >  5:325246/2‑146 (MQ=255)                                                                                                                            ttCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGACATTCCAGGCCCCGGCTTCCTCAACTTCTTCCAGaa              >  7:112914/1‑149 (MQ=255)                                                                                                                              cGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCCACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGGGGAGATTGCTGGCCCGGGCTTCCTCAACTGCTTCGAGcaca            >  4:250268/1‑149 (MQ=255)                                                                                                                                    cAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACg      <  3:155569/149‑1 (MQ=255)                                                                                                                                        aTCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCt  <  8:3986/149‑1 (MQ=255)                                                                                                                                          |                                                                                                                                                    AAAGACACCATTCGCCAGCTGATCCAGCAAGCCCTCACCCAACTCGTCACCGACGGTGTGCTGCCTGAAGGGCTGTCGCCGGCGATCCAGGTGGAAAACGCCCGGGACAAGACCCACGGCGACTTCGCCAGCAACATCGCCATGATGCTGGCCAAGCCGGCCGGCATGAA‑GCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCT  >  NC_002947/5813083‑5813366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑