Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,839,099	G→A	7.5%	A157T (GCC→ACC)	yheT →	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,839,099	0	G	A	7.5%	149.2 / 2.1	53	A157T (GCC→ACC)	yheT	putative hydrolase
Reads supporting (aligned to +/- strand):  ref base G (24/25);  new base A (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.42e-01

CCGCGCAGCTACCATTCCGGCGCCAGCGAAGACCTGGCGGAAATTGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAGGCGCATTTCATGCGCGAGATGCTGGCGTATGTTCAGCTCAAGCAGCG  >  NC_002947/5838952‑5839241                                                                                                                                                    |                                                                                                                                                ccGCGCAGCTACCATTCCGGCGCCAGCGAAGACCTGGCGGAAATTGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCgc                                                                                                                                                >  2:82667/1‑149 (MQ=255)  cgcgCAGCTACCATTCCGGCGCCAGCGAAGACCTGGCGGAAATTGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAACGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCgcc                                                                                                                                               <  8:70500/149‑1 (MQ=255)   gcgcAGCTACCATTCCGGCGCCAGCGAAGACCTGGCGGAAATTGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCgc                                                                                                                                                >  1:202097/1‑147 (MQ=255)         cTACCATTCCGGCGCCAGCGAAGACCTGGCGGAAATTGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAAc                                                                                                                                        >  1:351604/1‑149 (MQ=255)           aCCATTCCGGCGCCAGCGAAGACCTGGCGGAAATTGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTg                                                                                                                                      >  6:157130/1‑149 (MQ=255)           aCCATTCCGGCGCCAGCGAAGACCTGGCGGAAATTGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCACCAGCCAACTg                                                                                                                                      >  5:13785/1‑149 (MQ=255)           aCCATTCCGGCGCCAGCGAAGACCTGGCGGAAATTGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCACCAGCCAACTg                                                                                                                                      >  5:18832/1‑149 (MQ=255)             cATTCCGGCGCCAGCGAAGACCTGGCGGAAATTGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGa                                                                                                                                    <  2:202097/149‑1 (MQ=255)             aaTTCCTGCGCCAGCGAAGACCTGTCGGAAATTGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGa                                                                                                                                    <  8:337466/148‑1 (MQ=255)              aTTCCGGCGCCAGCGAAGACCTGGCGGAAATTGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTggtg                                                                                                                                   <  8:104790/149‑3 (MQ=255)                  cGGCGCCAGCGAAGACCTGGCGGAAATTGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTCTATGCAGTGGGTTATTCGCTGGGTGGCAATGTAATGTTGAAATAGCTGGGAGAGAGCGGCGTCGACATCCAACTGGAGGCAg                                                                                                                               >  8:326536/1‑149 (MQ=255)                      gcCAGCGAAGACCTGGCGGAAATTGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGc                                                                                                                           >  8:1408/1‑149 (MQ=255)                          gCGAAGACCTGGCGGAAATTGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCa                                                                                                                       <  2:97202/149‑1 (MQ=255)                                  cTGGCGGAAATTGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGt                                                                                                               <  1:82667/149‑1 (MQ=255)                                     gCGGAAATTGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTAGGTGcc                                                                                                            >  7:162321/1‑149 (MQ=255)                                      cGGAAATTGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGccc                                                                                                           <  2:351604/149‑1 (MQ=255)                                       ggAAATTGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGAGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAg                                                                                                                                   <  1:338585/124‑1 (MQ=255)                                       ggAAATTGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGAGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAg                                                                                                                                   >  2:338585/1‑124 (MQ=255)                                          aaTTGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCACCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTc                                                                                                       <  6:13785/149‑1 (MQ=255)                                          aaTTGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCACCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTc                                                                                                       <  6:18832/149‑1 (MQ=255)                                            ttGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCg                                                                                                                 >  7:287709/1‑137 (MQ=255)                                            ttGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTAGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCg                                                                                                                 <  8:287709/137‑1 (MQ=255)                                                 aGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTgg                                                                                                 <  5:157130/148‑1 (MQ=255)                                                    cATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCa                                                                                             >  4:170991/1‑149 (MQ=255)                                                     aTCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCAc                                                                                            >  7:305104/1‑149 (MQ=255)                                                          cgcgCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACt                                                                                           <  3:170991/145‑1 (MQ=255)                                                             gcCCAGCGGCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGa                                                                                    <  5:60524/149‑1 (MQ=255)                                                               ccAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGAcc                                                                                  <  2:304575/149‑1 (MQ=255)                                                                            gcgcCGCTGTATGCAGTGGGTTTTTCGCTGGGTGGCAATGTACTGTTCAAATACCTGGGAGAGAGCGTCGTCGCCAGACAACTGTAGGCAGCGGCTGCAGTTTCGTTGCTCTTTCGACTGGATCACTGTGTCGACCGTATCGGCCAAg                                                                      <  8:54346/148‑1 (MQ=255)                                                                            gcgcCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAgg                                                                     <  6:334426/149‑1 (MQ=255)                                                                                            tGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTa                                                     >  6:276867/1‑148 (MQ=255)                                                                                            tGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTAt                                                    >  4:237385/1‑149 (MQ=255)                                                                                               gTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAg                                                 >  3:33376/1‑149 (MQ=255)                                                                                                    tggCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAGGCGCa                                            >  2:186855/3‑149 (MQ=255)                                                                                                     cGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAGGCGCa                                            <  5:218237/148‑1 (MQ=255)                                                                                                      gCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAGGCGCAtt                                          >  1:205610/1‑149 (MQ=255)                                                                                                      gCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAGGCGCAtt                                          >  2:265474/1‑149 (MQ=255)                                                                                                        tGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAGGCGCAatt                                         >  2:283937/1‑148 (MQ=255)                                                                                                          ggTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAGGCGCATTTCa                                       <  1:186855/148‑1 (MQ=255)                                                                                                          ggTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAGGCGCATTTCAt                                      >  5:221700/1‑149 (MQ=255)                                                                                                          ggTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAGGCGCATTTCAt                                      <  7:1408/149‑1 (MQ=255)                                                                                                           gTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAGGCGCATTTCATg                                     <  1:81325/149‑1 (MQ=255)                                                                                                            tGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAGGCGCATTTCATgc                                    <  3:237385/149‑1 (MQ=255)                                                                                                                aaTGTACTGTTGAAATACCTGGGAGAGAGCCGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAGGCGCATTTCATgcgc                                  >  1:31902/1‑147 (MQ=255)                                                                                                                    tACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAGGCGCATTTCATGCGCGAGATg                            <  4:33376/149‑1 (MQ=255)                                                                                                                       tGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAGgc                                               <  5:236168/127‑1 (MQ=255)                                                                                                                       tGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAGgc                                               >  6:236168/1‑127 (MQ=255)                                                                                                                         ttGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGg‑t                                                        >  3:262567/1‑116 (MQ=255)                                                                                                                         ttGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGg‑t                                                        <  4:262567/116‑1 (MQ=255)                                                                                                                          tGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAGGCGCATTTCATGCGCGAGATGCTGGc                       <  8:162321/148‑1 (MQ=255)                                                                                                                           gAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAGgc                                               >  2:314018/1‑123 (MQ=255)                                                                                                                           gAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAGgc                                               <  1:314018/123‑1 (MQ=255)                                                                                                                                       agagagCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAGGCGCATTTCATGCGCGAGATGCTGGCGTATGTTCAGCTc          >  7:129110/1‑148 (MQ=255)                                                                                                                                        gagagCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGGCTGTATCAGGCGCATTTCATGCGCGAGATGCTGGCGTATGTTCAGCTCa         <  1:265474/149‑1 (MQ=255)                                                                                                                                             cggcgTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAGGCGCATTTCATGCGCGAGATGCTGGCGTATGTTCAGCTCAagcagc   >  3:142903/1‑149 (MQ=255)                                                                                                                                              ggcgTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAGGCGCATTTCATGCGCGAGATGCTGGCGTATGTTCAGCTCAAGCAGCg  >  6:316297/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                CCGCGCAGCTACCATTCCGGCGCCAGCGAAGACCTGGCGGAAATTGTCAGCCATCTTCGCGCCCAGCGCCCGCTGGCGCCGCTGTATGCAGTGGGTTATTCGCTGGGTGGCAATGTACTGTTGAAATACCTGGGAGAGAGCGGCGTCGCCAGCCAACTGGAGGCAGCGGCTGCAGTTTCGGTGCCCTTTCGCCTGGACCACTGTGCCGACCGTATCGGCCAAGGGTTCTCCAAGG‑TGTATCAGGCGCATTTCATGCGCGAGATGCTGGCGTATGTTCAGCTCAAGCAGCG  >  NC_002947/5838952‑5839241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑