Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,902,732	T→A	6.3%	K102M (AAG→ATG) ‡	PP_5175 ←	HlyD family secretion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,902,732	0	T	A	6.3%	178.9 / 2.7	63	K102M (AAG→ATG) ‡	PP_5175	HlyD family secretion protein
Reads supporting (aligned to +/- strand):  ref base T (31/27);  new base A (2/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.08e-01

CTTGAGTGCGCTCTGATTGCTGCGCAGCGCGGCCTCGGCAGCATCGTACTCGCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCT  >  NC_002947/5902584‑5902876                                                                                                                                                     |                                                                                                                                                  cTTGAGTGCGCTCTGATTGCTGCGCAGCGCGGCCTCGGCAGCATCGTACTCGCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCt                                                                                                                                                   >  5:71235/1‑149 (MQ=255)  ttGAGTGCGCTCTGATTGCTGCGCAGCGCGGCCTCGGCAGCATCGTACTCGCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCtt                                                                                                                                                  >  4:288656/1‑149 (MQ=255)  ttGAGTGCGCTCTGATTGCTGCGCAGCGCGGCCTCGGCAGCATCGTACTCGCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCtt                                                                                                                                                  >  4:85016/1‑149 (MQ=255)     aGTGCGGTCTGATTGCTGCGCAGCGCGGCCTCGGCAGCATCGTACTCGCTCTGGCTGGTGTCGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTgg                                                                                                                                                <  7:274177/148‑1 (MQ=255)       tGCGCTCTGATTGCTGCGCAGCGCGGCCTCGGCAGCATCGTACTCGCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGcc                                                                                                                                              >  1:310331/1‑148 (MQ=255)        gcgcTCTGATTGCTGCGCAGCGCGGCCTCGGCAGCATCGTACTCGCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGcc                                                                                                                                              >  1:24328/1‑147 (MQ=255)              tGATTGCTGCGCAGCGCGGCCTCGGCAGCATCGTACTCGCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGa                                                                                                                                       >  4:29918/1‑148 (MQ=255)              tGATTGCTGCGCAGCGCGGCCTCGGCAGCATCGTACTCGCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGa                                                                                                                                       >  6:73152/1‑148 (MQ=255)                aTTGCTGCGCAGCGCGGCCTCGGCAGCATCGTACTCGCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACa                                                                                                                                     <  1:34847/148‑1 (MQ=255)                    ctgcGCAGCGCGGCCTCGGCAGCATCGTACTCGCTCTGGCTGGTGTAGCCCTTGGCCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCTAATACCTCGGCCTTGGCCGAGTCGACATTGtt                                                                                                                                <  2:24328/149‑1 (MQ=255)                     tgcGCAGCGCGGCCTCGGCAGCATCGTACTCGCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTc                                                                                                                               >  5:49678/1‑149 (MQ=255)                     tgcGCAGCGCGGCCTCGGCAGCATCGTACTCGCTCTGGCTGGTGTAGCCCTTGCGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCAAGGCCGAg                                                                                                                                           <  7:228106/137‑1 (MQ=255)                              cgGCCTCGGCAGCATCGTACTCGCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCt                                                                                                                     <  3:72731/149‑1 (MQ=255)                               ggCCGCGGCAGTAGGGTACTCGCTCGGGGTGGTGTAGCCCTTGGGCATTTTTTTTTGCGGGCGCACTATGGCCGCTCTGGTCTGGGTGCCCGGCCCCGGCGGGGCTAATACCTGGGCCTGGGCCGACTCGACATTGTTCTGCAG‑GTCtt                                                                                                                    <  6:49678/149‑1 (MQ=255)                                 ccTCGGCAGCATCGTACTCGCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTc                                                                                                                  <  3:85016/149‑1 (MQ=255)                                  cTCGGCAGCATGGTACTCGCTCTGGCTGGTGTTCCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCg                                                                                                                 <  6:352892/149‑1 (MQ=255)                                     ggCAGCATCGTACTCGCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCggg                                                                                                               >  7:281818/1‑148 (MQ=255)                                        agcaTCGTACTCGCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCAAGGCCGAg                                                                                                                                           <  2:346617/118‑1 (MQ=255)                                        agcaTCGTACTCGCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCAAGGCCGAg                                                                                                                                           >  1:346617/1‑118 (MQ=255)                                          caTCGTACTCGCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTc                                                                                                             <  6:108463/145‑1 (MQ=255)                                          caTCGTACTCGCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTc                                                                                                             >  5:108463/1‑145 (MQ=255)                                            tactACTCTCTCTCCCTGCTGTAGCCCTTGGGCAGCACATTTTGCTCGCGCAAGAAGCCCGCACTGGTCTGGGTGACCCGCCCCTGCGCGTCGAATAACTCGGCCTTGGCAGAGTCGACAATCTTATGCAG‑ATCTATCCGGTCAAGccc                                                                                                       >  6:194704/4‑148 (MQ=255)                                               tACTCGCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGc                                                                                                    <  6:111819/149‑1 (MQ=255)                                                  tCGCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAg                                                                                                 >  2:109586/1‑149 (MQ=255)                                                  tCGCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACACGCGCCTGCGCGGCGAATACCTCGGCCTTGGCAGAGTCGACATTGTTCTTCAG‑GTCTTTCGGGTAAAGACGGGc                                                                                                    >  7:78130/1‑146 (MQ=255)                                                    gCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCa                                                                                               >  4:155385/1‑149 (MQ=255)                                                    gCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCa                                                                                               >  8:1333/1‑149 (MQ=255)                                                    gCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACATCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCa                                                                                               >  6:2643/1‑149 (MQ=255)                                                          gctggTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGCCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCCGGCGAGCACCTGGt                                                                                         >  5:237414/1‑149 (MQ=255)                                                            tggTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGAGATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTg                                                                                       >  6:334759/1‑149 (MQ=255)                                                              gtgtAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAGCGTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTgg                                                                                      <  1:235259/149‑1 (MQ=255)                                                                    ccgttGGGCAGCTGTTTTTGGTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGAc                                                                               <  6:71235/146‑1 (MQ=255)                                                                         gggCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGAt                                                                          >  3:353085/1‑149 (MQ=255)                                                                          ggCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGAt                                                                          <  3:155385/148‑1 (MQ=255)                                                                           gcagcaGTTTTTGCTGGCGCACGAAGGCGGCACTGGGCTGGGTGACCCGCGCCTGCGCGGCTAATACCTCGGCATTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGAc                                                                               <  4:252842/142‑1 (MQ=255)                                                                           gaagcagTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGAGGCGAATACCTCGGCCTTTGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGATATCg                                                                        >  3:252842/3‑149 (MQ=255)                                                                              gcagTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGccc                                                                     <  5:73152/149‑1 (MQ=255)                                                                                      tGCTGGCGCACGTAGGCGGCACTGGTCTTGGTGACCGGCGCCTTCGCGGCTAATACCTCTGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGTGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGGGCGATCACGTcaa                                                              <  8:78130/148‑3 (MQ=255)                                                                                             gcACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTAcgc                                                      >  5:137056/1‑149 (MQ=255)                                                                                             gcACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTAcgc                                                      >  1:85294/1‑149 (MQ=255)                                                                                              cACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTAcgc                                                      >  6:167464/1‑148 (MQ=255)                                                                                              cACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGACGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGc                                                                                                    >  3:111237/1‑102 (MQ=255)                                                                                              cACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGACTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAACCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCAAGTCGACGCTAcgc                                                      >  3:337508/1‑148 (MQ=255)                                                                                                 gAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGaaa                                                  <  7:1333/149‑1 (MQ=255)                                                                                                  aaGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAAt                                                 <  5:167464/149‑1 (MQ=255)                                                                                                   aGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAAt                                                 >  4:219505/1‑148 (MQ=255)                                                                                                        gcgtgggTCTGGTGGCCCGGGGCCTGCGGGGCGAAGCCCTGGGCGGTGGCGGCGTCGACATTGTGCTGCAG‑TGCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCtt                                           <  6:41463/144‑1 (MQ=255)                                                                                                          aCTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCGTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTgc                                         <  8:281818/149‑1 (MQ=255)                                                                                                          aCTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTgc                                         >  3:222937/1‑149 (MQ=255)                                                                                                              gTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTgccgc                                      <  2:310331/148‑1 (MQ=255)                                                                                                                 tGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGAc                                  >  7:224372/1‑149 (MQ=255)                                                                                                                     cgccccGCGCCTGCGCGGCTTTTACGGGGGCCTTGGCCGTTTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCAGCTGGTTGGGCTTTACTTGATCGCGCACGTCTACGCTACGCGAAATTATCTTGCCGCCGACGCgg                              <  6:237414/146‑1 (MQ=255)                                                                                                                       aCCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGaa                            >  2:208229/1‑149 (MQ=255)                                                                                                                        cccGCGCCGGCGCGGCTAATACCTCGGCCTGGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAAt                           <  4:353085/149‑1 (MQ=255)                                                                                                                         ccGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAAt                           >  7:258374/1‑148 (MQ=255)                                                                                                                           gcgcCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGAc                        <  1:208229/149‑1 (MQ=255)                                                                                                                            cgcCTGCGCGGCTAAACCCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACa                       <  8:224372/149‑1 (MQ=255)                                                                                                                                  cggggccAATCCCTCGGCCTTGGCCTTGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATc                   <  4:337508/144‑1 (MQ=255)                                                                                                                                   gcggcgAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCgg                 >  5:155568/1‑148 (MQ=255)                                                                                                                                    cggcgAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTc               <  1:109586/149‑1 (MQ=255)                                                                                                                                     ggcgAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCt              <  3:288656/149‑1 (MQ=255)                                                                                                                                       cgAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGc            >  4:260793/1‑149 (MQ=255)                                                                                                                                        gAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCa           >  3:264896/1‑149 (MQ=255)                                                                                                                                              cTCGGCCAAGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGCAATGACAAATCGGTCTGCACCcgcg     >  5:216548/1‑149 (MQ=255)                                                                                                                                                 ggCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCTAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCTGAATGACAAATCGGTCTGCACCCgcgc    <  5:2643/147‑1 (MQ=255)                                                                                                                                                 ggCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCt  >  1:68112/1‑149 (MQ=255)                                                                                                                                                     ttGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCt              >  6:5453/1‑133 (MQ=255)                                                                                                                                                     ttGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCt              <  5:5453/133‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CTTGAGTGCGCTCTGATTGCTGCGCAGCGCGGCCTCGGCAGCATCGTACTCGCTCTGGCTGGTGTAGCCCTTGGGCAGCAGTTTTTGCTGGCGCACGAAGGCCGCACTGGTCTGGGTGACCCGCGCCTGCGCGGCGAATACCTCGGCCTTGGCCGAGTCGACATTGTTCTGCAG‑GTCTTTCGGGTCAAGCCGGGCGAGCACCTGGTTGGCCTTGACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCT  >  NC_002947/5902584‑5902876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑