Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,917,643 A→T 6.4% Q234L (CAG→CTG)  ygiF → thiamine triphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,917,6430AT 6.4% 178.7 / 2.7 62Q234L (CAG→CTG) ygiFthiamine triphosphatase
Reads supporting (aligned to +/- strand):  ref base A (31/27);  new base T (2/2);  total (33/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.75e-01

CCACTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCACCC  >  NC_002947/5917504‑5917786
                                                                                                                                           |                                                                                                                                               
ttgcTGATGTCGCACGGCATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTggg                                                                                                                                        >  5:336387/4‑149 (MQ=255)
ccACTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTggg                                                                                                                                        <  6:263554/149‑1 (MQ=255)
 cACTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTggg                                                                                                                                        >  5:119828/1‑148 (MQ=255)
  aCTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTggg                                                                                                                                        >  1:210364/1‑147 (MQ=255)
  aCTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGgca                                                                                                                                      <  4:306166/149‑1 (MQ=255)
    tGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGgcag                                                                                                                                     >  8:62953/1‑148 (MQ=255)
      aTGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGgcagc                                                                                                                                    <  6:88269/147‑1 (MQ=255)
            tGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCTGCTGCCCAGCAGctgccaggc                                                                                                                            <  6:232937/149‑3 (MQ=255)
            tGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAgcg                                                                                                                            >  8:242615/1‑149 (MQ=255)
               gACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCt                                                                                                                         <  7:88436/149‑1 (MQ=255)
               gACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCt                                                                                                                         >  6:106061/1‑149 (MQ=255)
                     aGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAgctgc                                                                                                                                            <  4:335079/124‑1 (MQ=255)
                     aGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAgctgc                                                                                                                                            >  3:335079/1‑124 (MQ=255)
                     aGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGa                                                                                                                   <  2:210364/149‑1 (MQ=255)
                       cAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGAGACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCCAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTCGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAg                                                                                                                  >  6:81718/1‑148 (MQ=255)
                         aGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGctgctg                                                                                                                                          >  7:198358/1‑122 (MQ=255)
                         aGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGctgctg                                                                                                                                          <  8:198358/122‑1 (MQ=255)
                         aGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAg                                                                                                               >  8:249299/1‑149 (MQ=255)
                           gCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCagcagc                                                                                                                                 >  5:216526/1‑129 (MQ=255)
                           gCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCagcagc                                                                                                                                 <  6:216526/129‑1 (MQ=255)
                             tGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAgctgct                                                                                                                                           >  4:118509/1‑117 (MQ=255)
                             tGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAgctgct                                                                                                                                           <  3:118509/117‑1 (MQ=255)
                                   cgGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCcac                                                                                                      >  7:159008/1‑148 (MQ=255)
                                         ccGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGt                                                                                                              <  4:290190/134‑1 (MQ=255)
                                         ccGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGt                                                                                                              >  3:290190/1‑134 (MQ=255)
                                         ccGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGc                                                                                                <  5:106061/148‑1 (MQ=255)
                                          cGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACGGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTgg                                                                                                                       >  1:75098/1‑124 (MQ=255)
                                          cGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTgg                                                                                                                       <  2:75098/124‑1 (MQ=255)
                                          cGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCa                                                                                              >  4:257865/1‑149 (MQ=255)
                                             ctgctgGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCTGCTGCCCAGCAGctgccag                                                                                                                              <  1:122286/114‑1 (MQ=255)
                                             ctgctgGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCTGCTGCCCAGCAGctgccag                                                                                                                              >  2:122286/1‑114 (MQ=255)
                                                  ggAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGcc                                                                                      <  8:159008/149‑1 (MQ=255)
                                                       cGGACAGCTACGAGCTGGACCTGCCGCACCCCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACACCGGCCACTGGCGCCTGCTc                                                                                 >  5:178223/1‑149 (MQ=255)
                                                       cGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTc                                                                                 <  3:348683/149‑1 (MQ=255)
                                                          aCAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAg                                                                              >  4:93553/1‑149 (MQ=255)
                                                              cTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACt                                                                          <  7:242615/149‑1 (MQ=255)
                                                              cTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACt                                                                          >  5:73360/1‑149 (MQ=255)
                                                              cTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACt                                                                          <  7:62953/149‑1 (MQ=255)
                                                                 cGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTg                                                                         <  2:123083/147‑1 (MQ=255)
                                                                    gCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGCCTGGGTc                                                                     >  7:264109/1‑148 (MQ=255)
                                                                    gCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTc                                                                     >  1:263442/1‑148 (MQ=255)
                                                                        gACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGt                                                                 <  6:336387/148‑1 (MQ=255)
                                                                         aCCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGc                                                               >  7:280576/1‑149 (MQ=255)
                                                                           cTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCt                                                             >  5:190440/1‑149 (MQ=255)
                                                                           cTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCt                                                             >  5:195517/1‑149 (MQ=255)
                                                                               cGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTgagc                                                         <  8:191589/149‑1 (MQ=255)
                                                                                    acCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTgagcgag                                                      >  3:186535/1‑147 (MQ=255)
                                                                                    acCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTgagcgag                                                      <  4:186535/147‑1 (MQ=255)
                                                                                     ccGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTg                                                   >  8:42448/1‑149 (MQ=255)
                                                                                                gCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCGAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCAc                                        >  8:231582/1‑149 (MQ=255)
                                                                                                gCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCAc                                        <  6:73360/149‑1 (MQ=255)
                                                                                                          cccTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTg                              <  3:93553/149‑1 (MQ=255)
                                                                                                               gacgacGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGc                           <  6:195517/147‑1 (MQ=255)
                                                                                                               gacgacGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGc                           <  6:190440/147‑1 (MQ=255)
                                                                                                                 cgacgCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAgg                       <  7:249299/149‑1 (MQ=255)
                                                                                                                        tATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCAcc                >  5:124313/1‑149 (MQ=255)
                                                                                                                         aTGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACcg               >  3:70585/1‑149 (MQ=255)
                                                                                                                                ccTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGgc                      <  3:24731/135‑1 (MQ=255)
                                                                                                                                ccTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGgc                      >  4:24731/1‑135 (MQ=255)
                                                                                                                                 cTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTgagcga                                                       <  2:110324/101‑1 (MQ=255)
                                                                                                                                 cTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTgagcga                                                       >  1:110324/1‑101 (MQ=255)
                                                                                                                                      cTGGCTGCTGCCCAGCAGCTGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCAccc  >  8:185254/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                               
CCACTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCACCC  >  NC_002947/5917504‑5917786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑