Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,917,649 T→C 7.1% L236P (CTG→CCG) ‡ ygiF → thiamine triphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,917,6490TC 7.1% 158.9 / 2.5 56L236P (CTG→CCG) ‡ygiFthiamine triphosphatase
Reads supporting (aligned to +/- strand):  ref base T (28/24);  new base C (2/2);  total (30/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.34e-01

CCACTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCACCCGCGACCTGCG  >  NC_002947/5917504‑5917796
                                                                                                                                                 |                                                                                                                                                   
ttgcTGATGTCGCACGGCATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTggg                                                                                                                                                  >  5:336387/4‑149 (MQ=255)
ccACTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTggg                                                                                                                                                  <  6:263554/149‑1 (MQ=255)
 cACTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTggg                                                                                                                                                  >  5:119828/1‑148 (MQ=255)
  aCTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTggg                                                                                                                                                  >  1:210364/1‑147 (MQ=255)
  aCTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGgca                                                                                                                                                <  4:306166/149‑1 (MQ=255)
    tGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGgcag                                                                                                                                               >  8:62953/1‑148 (MQ=255)
      aTGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGgcagc                                                                                                                                              <  6:88269/147‑1 (MQ=255)
            tGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCTGCTGCCCAGCAGctgccaggc                                                                                                                                      <  6:232937/149‑3 (MQ=255)
            tGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAgcg                                                                                                                                      >  8:242615/1‑149 (MQ=255)
               gACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCt                                                                                                                                   <  7:88436/149‑1 (MQ=255)
               gACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCt                                                                                                                                   >  6:106061/1‑149 (MQ=255)
                     aGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGa                                                                                                                             <  2:210364/149‑1 (MQ=255)
                       cAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGAGACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCCAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTCGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAg                                                                                                                            >  6:81718/1‑148 (MQ=255)
                         aGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGctgctg                                                                                                                                                    >  7:198358/1‑122 (MQ=255)
                         aGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGctgctg                                                                                                                                                    <  8:198358/122‑1 (MQ=255)
                         aGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAg                                                                                                                         >  8:249299/1‑149 (MQ=255)
                           gCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCagcagc                                                                                                                                           <  6:216526/129‑1 (MQ=255)
                           gCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCagcagc                                                                                                                                           >  5:216526/1‑129 (MQ=255)
                             tGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAgctgct                                                                                                                                                     <  3:118509/117‑1 (MQ=255)
                             tGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAgctgct                                                                                                                                                     >  4:118509/1‑117 (MQ=255)
                                   cgGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCcac                                                                                                                >  7:159008/1‑148 (MQ=255)
                                         ccGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGt                                                                                                                        >  3:290190/1‑134 (MQ=255)
                                         ccGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGt                                                                                                                        <  4:290190/134‑1 (MQ=255)
                                         ccGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGc                                                                                                          <  5:106061/148‑1 (MQ=255)
                                          cGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACGGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTgg                                                                                                                                 >  1:75098/1‑124 (MQ=255)
                                          cGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTgg                                                                                                                                 <  2:75098/124‑1 (MQ=255)
                                          cGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCa                                                                                                        >  4:257865/1‑149 (MQ=255)
                                             ctgctgGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCTGCTGCCCAGCAGctgccag                                                                                                                                        <  1:122286/114‑1 (MQ=255)
                                             ctgctgGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCTGCTGCCCAGCAGctgccag                                                                                                                                        >  2:122286/1‑114 (MQ=255)
                                                  ggAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGcc                                                                                                <  8:159008/149‑1 (MQ=255)
                                                       cGGACAGCTACGAGCTGGACCTGCCGCACCCCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACACCGGCCACTGGCGCCTGCTc                                                                                           >  5:178223/1‑149 (MQ=255)
                                                       cGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTc                                                                                           <  3:348683/149‑1 (MQ=255)
                                                          aCAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAg                                                                                        >  4:93553/1‑149 (MQ=255)
                                                              cTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACt                                                                                    >  5:73360/1‑149 (MQ=255)
                                                              cTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACt                                                                                    <  7:62953/149‑1 (MQ=255)
                                                              cTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACt                                                                                    <  7:242615/149‑1 (MQ=255)
                                                                 cGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTg                                                                                   <  2:123083/147‑1 (MQ=255)
                                                                    gCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGCCTGGGTc                                                                               >  7:264109/1‑148 (MQ=255)
                                                                    gCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTc                                                                               >  1:263442/1‑148 (MQ=255)
                                                                        gACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGt                                                                           <  6:336387/148‑1 (MQ=255)
                                                                         aCCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGc                                                                         >  7:280576/1‑149 (MQ=255)
                                                                           cTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCt                                                                       >  5:195517/1‑149 (MQ=255)
                                                                           cTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCt                                                                       >  5:190440/1‑149 (MQ=255)
                                                                               cGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTgagc                                                                   <  8:191589/149‑1 (MQ=255)
                                                                                    acCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTgagcgag                                                                <  4:186535/147‑1 (MQ=255)
                                                                                    acCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTgagcgag                                                                >  3:186535/1‑147 (MQ=255)
                                                                                     ccGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTg                                                             >  8:42448/1‑149 (MQ=255)
                                                                                                gCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCGAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCAc                                                  >  8:231582/1‑149 (MQ=255)
                                                                                                gCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCAc                                                  <  6:73360/149‑1 (MQ=255)
                                                                                                          cccTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTg                                        <  3:93553/149‑1 (MQ=255)
                                                                                                               gacgacGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGc                                     <  6:195517/147‑1 (MQ=255)
                                                                                                               gacgacGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGc                                     <  6:190440/147‑1 (MQ=255)
                                                                                                                 cgacgCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAgg                                 <  7:249299/149‑1 (MQ=255)
                                                                                                                        tATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCAcc                          >  5:124313/1‑149 (MQ=255)
                                                                                                                         aTGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACcg                         >  3:70585/1‑149 (MQ=255)
                                                                                                                                ccTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGgc                                <  3:24731/135‑1 (MQ=255)
                                                                                                                                ccTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGgc                                >  4:24731/1‑135 (MQ=255)
                                                                                                                                 cTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTgagcga                                                                 <  2:110324/101‑1 (MQ=255)
                                                                                                                                 cTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTgagcga                                                                 >  1:110324/1‑101 (MQ=255)
                                                                                                                                      cTGGCTGCTGCCCAGCAGCTGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCAccc            >  8:185254/1‑149 (MQ=255)
                                                                                                                                               gctgGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCACCCGCGACCTgc   >  1:207154/1‑149 (MQ=255)
                                                                                                                                                ctgGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCACCCGCGACCTgcg  >  3:324912/1‑149 (MQ=255)
                                                                                                                                                ctgGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCACCCGCGACCTgc   <  2:263442/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CCACTGATGCCGTGCGACATCAGCAAGGCTGAGCGCGGTTACCGCCTGCTGGAACCGGACAGCTACGAGCTGGACCTGCCGCACACCGCGCTGGACGCCGAAATGGCCCTGGACGACGCCTATGCCGCCCTCGCCTGGCAGCTGCTGGGCAGCAGCCAGCGCCTGGCCGAGCAGTACCGCCACAACGGCCACTGGCGCCTGCTCCAGGACTGGGTCGAGTGCCTGAGCGAGCTGCGCGCGCTCACCGCCAGCCTGGGCCAGGCAGCACCGCGCGCCACCACCCGCGACCTGCG  >  NC_002947/5917504‑5917796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑